Isolation, Characterization and Biosafety Evaluation of Lactobacillus Fermentum OK with Potential Oral Probiotic Properties

Mann, So-Yon; Park, Myeong Soo; Johnston, Tony V.; Ji, Geun Eog; Hwang, Keum Taek; Ku, Seockmo

doi:10.1007/s12602-021-09761-z

Cited by 17 publications

(15 citation statements)

References 95 publications

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“…28 L. fermentum and L. gasseri can release anti-microbial agents such as bacteriocins, 29,30 biosurfactant 31,32 and hydrogen peroxide. 33,34 In line with this, our results showed that LF23 and LG80 fermentation supernatants could significantly inhibit the growth of H. pylori and reduce H. pylori infection in vivo, indicating that they can metabolize and produce bacteriostatic substances. In the case of excluding the influence of acid, the fermentation supernatant of LAB can also significantly inhibit the growth of H. pylori, indicating that in addition to the secretion of organic acids, it can also produce other antibacterial substances.…”

Section: Discussionsupporting

confidence: 83%

Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Zhao

et al. 2022

Food Funct.

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Section: Discussionsupporting

confidence: 83%

Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Zhao

et al. 2022

Food Funct.

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“…A similar method was also used by Gönczi et al, who found that L. rhamnosus and L. casei were effective strains for F. nucleatum and P. gingivalis containment [ 29 ]. The L. fermentum OK strain, when co-cultured with F. nucleatum and P. gingivalis , significantly inhibited their growth, although its proliferation was also retarded [ 30 ]. L. fermentum SG-A95 and L. salivarius SG-M6 were used both as viable strains and CFSs against P. gingivalis , being able to significantly reduce its growth in agar disc diffusion and broth dilution methods.…”

Section: Resultsmentioning

confidence: 99%

“…To our knowledge, this is the first paper reporting on viable probiotics and their CFS possible interactions with F. nucleatum and P. gingivalis co-aggregation. In fact, in the literature, it was only found that the L. fermentum OK strain co-aggregated efficiently with the single P. gingivalis pathogen [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Growth Conditions Influence Lactobacillus Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens Fusobacterium nucleatum and Porphyromonas gingivalis

et al. 2023

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Fusobacterium nucleatum and Porphyromonas gingivalis human periodontopathogens play a leading part in oral squamous cell carcinoma through cell proliferation, invasion, and persistent inflammation promotion and maintenance. To explore how the activity of Lactobacillus-derived cell-free supernatants (CFSs) can be influenced by growth medium components, CFSs were produced both in the standard MRS and the novel animal-derivative-free “Terreno Industriale Lattobacilli” (TIL) media, and in vitro screened for the containment of F. nucleatum and P. gingivalis both single and co-cultured and also for the interference on their co-aggregation. The viability assay demonstrated that the Limosilactobacillus reuteri LRE11 and Ligilactobacillus salivarius LS03 MRS-produced CFSs were significantly more effective against single and co-cultured pathogens. All the other CFSs significantly improved their efficacy when produced in TIL. Both MRS- and TIL-produced CFSs significantly inhibited the single and co-cultured pathogen biofilm formation. Only Levilactobacillus brevis LBR01 CFS in MRS specifically reduced F. nucleatum and P. gingivalis co-aggregation, while viable LBR01, LS03, and LRE11 in MRS significantly co-aggregated with the pathogens, but only LS03 cultivated in TIL improved this effect. This work paves the way to better consider environmental growth conditions when screening for probiotic and postbiotic efficacy as crucial to pathogen aggregation, adhesion to the host’s niches, and exclusion.

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“…Instead, it is believed that the disease is a consequence of a microbially driven sharp pH drop of the dental biofilms, resulting in a dysbiotic shift of the resident dental microbiota toward acid-tolerating microorganisms, including the lactobacilli. The role of L. fermentum in the mouth is debated, and studies suggest that the bacterium have both positive (Terai et al, 2015;Mann et al, 2021) and negative health effects (Marchant et al, 2001;Lapirattanakul et al, 2020). Salivary MUC5B serves as the most important nutrient supply for the oral microbiome, satisfying the microbial community demand for carbon and nitrogen (Wickström et al, 2009a).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a highly conserved MUC5B-degrading protease, MdpL, from Limosilactobacillus fermentum

Leo

Svensäter²,

Lood³

et al. 2023

Front. Microbiol.

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MUC5B is the predominant glycoprotein in saliva and is instrumental in the establishment and maintenance of multi-species eubiotic biofilms in the oral cavity. Investigations of the aciduric Lactobacillaceae family, and its role in biofilms emphasizes the diversity across different genera of the proteolytic systems involved in the nutritional utilization of mucins. We have characterized a protease from Limosilactobacillus fermentum, MdpL (Mucin degrading protease from Limosilactobacillus) with a high protein backbone similarity with commensals that exploit mucins for attachment and nutrition. MdpL was shown to be associated with the bacterial cell surface, in close proximity to MUC5B, which was sequentially degraded into low molecular weight fragments. Mapping the substrate preference revealed multiple hydrolytic sites of proteins with a high O-glycan occurrence, although hydrolysis was not dependent on the presence of O-glycans. However, since proteolysis of immunoglobulins was absent, and general protease activity was low, a preference for glycoproteins similar to MUC5B in terms of glycosylation and structure is suggested. MdpL preferentially hydrolyzed C-terminally located hydrophobic residues in peptides larger than 20 amino acids, which hinted at a limited sequence preference. To secure proper enzyme folding and optimal conditions for activity, L. fermentum incorporates a complex system that establishes a reducing environment. The importance of overall reducing conditions was confirmed by the activity boosting effect of the added reducing agents L-cysteine and DTT. High activity was retained in low to neutral pH 5.5–7.0, but the enzyme was completely inhibited in the presence of Zn2+. Here we have characterized a highly conserved mucin degrading protease from L. fermentum. MdpL, that together with the recently discovered O-glycanase and O-glycoprotease enzyme groups, increases our understanding of mucin degradation and complex biofilm dynamics.

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Isolation, Characterization and Biosafety Evaluation of Lactobacillus Fermentum OK with Potential Oral Probiotic Properties

Cited by 17 publications

References 95 publications

Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Growth Conditions Influence Lactobacillus Cell-Free Supernatant Impact on Viability, Biofilm Formation, and Co-Aggregation of the Oral Periodontopathogens Fusobacterium nucleatum and Porphyromonas gingivalis

Characterization of a highly conserved MUC5B-degrading protease, MdpL, from Limosilactobacillus fermentum

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