Isolation, characterisation and detection of breath-derived extracellular vesicles

Dobhal, Garima; Datta, Amrita; Ayupova, Deanna; Teesdale‐Spittle, Paul; Goreham, Renee V.

doi:10.1038/s41598-020-73243-5

Cited by 19 publications

(10 citation statements)

References 18 publications

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“…Several methods have been developed for isolating exosomes and detecting exosomal proteins and nucleic acids (Fig. 2D) [196][197][198]. Exosome isolation and enrichment methods are based on exosome characteristics such as density, size, surface composition, and exosome precipitation [194,199].…”

Section: Detection Techniques Of Exosomesmentioning

confidence: 99%

Clinical application and detection techniques of liquid biopsy in gastric cancer

Zhou

et al. 2023

Mol Cancer

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Gastric cancer (GC) is one of the most common tumors worldwide and the leading cause of tumor-related mortality. Endoscopy and serological tumor marker testing are currently the main methods of GC screening, and treatment relies on surgical resection or chemotherapy. However, traditional examination and treatment methods are more harmful to patients and less sensitive and accurate. A minimally invasive method to respond to GC early screening, prognosis monitoring, treatment efficacy, and drug resistance situations is urgently needed. As a result, liquid biopsy techniques have received much attention in the clinical application of GC. The non-invasive liquid biopsy technique requires fewer samples, is reproducible, and can guide individualized patient treatment by monitoring patients' molecular-level changes in real-time. In this review, we introduced the clinical applications of circulating tumor cells, circulating free DNA, circulating tumor DNA, non-coding RNAs, exosomes, and proteins, which are the primary markers in liquid biopsy technology in GC. We also discuss the current limitations and future trends of liquid biopsy technology as applied to early clinical biopsy technology.

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Section: Detection Techniques Of Exosomesmentioning

confidence: 99%

Clinical application and detection techniques of liquid biopsy in gastric cancer

Zhou

et al. 2023

Mol Cancer

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“…Moreover, since cancerous exosomes represent only a small fraction of all exosomes present in body fluids, ultrasensitive and specific detection is a prerequisite for the development of exosome-based cancer diagnostics. To date, a variety of methods have been developed for exosome isolation as well as the detection of exosomal proteins and nucleic acids [ 21 – 26 ]. Although notable progress has been made, the limited sensitivity and specificity, low purity and throughput remain significant challenges for academic research and practical use [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomes as a new frontier of cancer liquid biopsy

et al. 2022

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Liquid biopsy, characterized by minimally invasive detection through biofluids such as blood, saliva, and urine, has emerged as a revolutionary strategy for cancer diagnosis and prognosis prediction. Exosomes are a subset of extracellular vesicles (EVs) that shuttle molecular cargoes from donor cells to recipient cells and play a crucial role in mediating intercellular communication. Increasing studies suggest that exosomes have a great promise to serve as novel biomarkers in liquid biopsy, since large quantities of exosomes are enriched in body fluids and are involved in numerous physiological and pathological processes. However, the further clinical application of exosomes has been greatly restrained by the lack of high-quality separation and component analysis methods. This review aims to provide a comprehensive overview on the conventional and novel technologies for exosome isolation, characterization and content detection. Additionally, the roles of exosomes serving as potential biomarkers in liquid biopsy for the diagnosis, treatment monitoring, and prognosis prediction of cancer are summarized. Finally, the prospects and challenges of applying exosome-based liquid biopsy to precision medicine are evaluated.

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“…The examination of bioinformatic components carried by circulating tumor exosomes, such as nucleic acids and proteins, can provide effective information for cancer diagnosis, monitoring and prognosis prediction. At present, a variety of methods have been established for exosome isolation and detection of exosome content [4][5][6][7][8][9], but there are still issues that prevent exosomes from being used clinically, including the complicated isolation of exosomes and the absence of tumor-speci c markers [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

Sun

Zhang

et al. 2023

Preprint

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Background: Exosomes have great potential as new biomarkers in liquid biopsy. However, due to the limitations of exosome extraction and component analysis procedures, further clinical applications of exosomes are hampered. Carcinoembryonic antigen (CEA) is a commonly used broad-spectrum tumor marker that is strongly expressed in a variety of malignancies. Results: In this study, CEA+ exosomes were directly separated from serum using immunomagnetic beads, and the nucleic acid to protein ultraviolet absorption ratio (NPr) of CEA+ exosomes was determined. It was found that the NPr of CEA+ exosomes in tumor group was higher than that of healthy group. We further analyzed the exosome-derived nucleic acid components using fluorescent staining and found that the concentration ratio of double-stranded DNA to protein (dsDPr) in CEA+ exosomes was also significantly different between the two groups, with a sensitivity of 100% and a specificity of 41.67% for the diagnosis of pan-cancer. The AUC of dsDPr combined with NPr was 0.87 and the ACU of dsDPr combined with CA242 could reach 0.94, showing good diagnostic performance for pan-cancer. Conclusions: This study demonstrates that the dsDPr of CEA+ exosomes can effectively distinguish exosomes derived from tumor patients and healthy individuals, which can be employed as a simple and cost-effective non-invasive screening technology to assist tumor diagnosis.

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Isolation, characterisation and detection of breath-derived extracellular vesicles

Cited by 19 publications

References 18 publications

Clinical application and detection techniques of liquid biopsy in gastric cancer

Clinical application and detection techniques of liquid biopsy in gastric cancer

Exosomes as a new frontier of cancer liquid biopsy

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

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