Isolation and wall analysis of dimorphic mutants of<i>Paracoccidioides brasiliensis</i>

Hallak, Joseph; San-Blas, Felipe; San-Blas, Gioconda

doi:10.1080/00362178285380081

Cited by 25 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, as demonstrated by Kanetsuma et al . (1974) and Hallak et al . (1982) in the transition process from the mycelial to the yeast form, the enzyme β‐glucan synthetase induces a change in the quaternary structure of this molecule.…”

Section: Discussionmentioning

confidence: 97%

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Medeiros

Silva

Malheiro

et al. 1999

British J Pharmacology

View full text Add to dashboard Cite

1 The in¯ammatory cell in¯ux towards the peritoneal cavity in mice inoculated i.p. with live or dead Histoplasma capsulatum or with its subcellular preparations was studied. We also evaluated the e ects of dexamethasone (Dexa) or MK886, an inhibitor of leukotriene (LT) biosynthesis, on the recruitment of leukocytes. 2 Live yeast form of fungus (LYH) induced an increase in neutrophils (NE) which was highest 4 to 24 h after inoculation. Mononuclear cell (MN) migration beginning at 24 h with a gradual increase over 48 and 168 h, and an eosinophil (EO) recruitment occurs between 24 and 48 h. 3 NE and EO recruitment induced by dead mycelial form of fungus (DMH) was greater than that observed for dead yeast form of fungus (DYH). A similar leukocyte migration pattern was seen after i.p. injection of the alkali-insoluble fraction (F1) from DYH (F1Y) and F1 from DMH (F1M) this being more active than former. The di erence in concentration of b-glucan in DYH and DMH could explain the di erent in¯ammatory capacity exhibited by the two forms of H. capsulatum. 4 LT seems to be the principal mediator of leukocyte migration in response to LYH, DYH or DMH or to b-glucan. However, other mediators appear to contribute to NE and EO migration since the treatment with Dexa was more e ective in inhibiting cell migration than MK886.

show abstract

Section: Discussionmentioning

confidence: 97%

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Medeiros

Silva

Malheiro

et al. 1999

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Established virulence factors include: 1) the ability to grow at 37°C and physiological pH[3,10] 2) secretion of many proteases, distinctive of Aspergillus species[11-13] 3) production of a thick polysaccharide capsule and the synthesis of melanin, such as in Cryptococcus species[14] 4) production of 1,3-glucan carbohydrate polymer, a virulence factor in Paracoccidioides brasiliensis and Histoplasma capsulatum [15-17] 5) having a spore size, which is comparable to alveolar space dimensions; this is particularly important for fungi that cause infection by inhalation 6) secretion of phospholipase enzymes in Candida species , C. neoformans , and A. fumigatus [18,19] and, 7) modulation of lipid metabolism, characteristic of Cryptococcus species and dimorphic fungi[20] [21]. …”

Section: Lipids As Fungal Virulence Factorsmentioning

confidence: 99%

Plasma membrane lipids and their role in fungal virulence

Rella

Farnoud

Poeta

2016

Progress in Lipid Research

View full text Add to dashboard Cite

There has been considerable evidence in recent years suggesting that plasma membrane lipids are important regulators of fungal pathogenicity. Various glycolipids have been shown to impart virulent properties in several fungal species, while others have been shown to play a role in host defense. In addition to their role as virulence factors, lipids also contribute to other virulence mechanisms such as drug resistance, biofilm formation, and release of extracellular vesicles. In addition, lipids also affect the mechanical properties of the plasma membrane through the formation of packed microdomains composed mainly of sphingolipids and sterols. Changes in the composition of lipid microdomains has been shown to disrupt the localization of virulence factors and affect fungal pathogenicity. This review gathers evidence on the various roles of plasma membrane lipids in fungal virulence and how lipids might contribute to the different processes that occur during infection and treatment. Insight into the role of lipids in fungal virulence can lead to an improved understanding of the process of fungal pathogenesis and the development of new lipid-mediated therapeutic strategies.

show abstract

“…The round yeast shape was also kept in a mutant strain where most α-glucan was replaced by 1,3-mannan, as well as in the parent strain that presented only reduced amounts of α-glucan due to the effect of multiple sub-culturing (San Blas et al, 1976, 1977b). Later analysis of cell wall polysaccharides proved that their composition, however, could vary not only with culture conditions, but also among isolates (San Blas and Vernet, 1977; San Blas and San Blas, 1982; San Blas et al, 1984), and especially in mutants with defects in phase transition (San Blas et al, 1977a; Hallak et al, 1982; San Blas and San Blas, 1992). At that time, these mutants were useful to review the models of dimorphism (Kanetsuna et al, 1972; Kanetsuna, 1981; San Blas and San Blas, 1984, 1994).…”

mentioning

confidence: 99%

“…Although a role for α-glucan in the yeast shape was discarded, its relationship with fungal virulence has long been speculated, especially because decreased virulence and loss of externally arranged α-glucan have been observed to occur concomitantly (San Blas et al, 1976, 1977b; San Blas and Vernet, 1977; Goihman-Yahr et al, 1980; Hallak et al, 1982; Brummer et al, 1990; Kashino et al, 1990). The role of fungal cell wall polysaccharides as pathogen-associated molecular patterns (PAMPs) has been widely explored in Candida albicans (Netea et al, 2008).…”

mentioning

confidence: 99%

The Paracoccidioides Cell Wall: Past and Present Layers Toward Understanding Interaction with the Host

Puccia¹,

Vallejo²,

Matsuo³

et al. 2011

Front. Microbio.

View full text Add to dashboard Cite

The cell wall of pathogenic fungi plays import roles in the interaction with the host, so that its composition and structure may determine the course of infection. Here we present an overview of the current and past knowledge on the cell wall constituents of Paracoccidioides brasiliensis and P. lutzii. These are temperature-dependent dimorphic fungi that cause paracoccidioidomycosis, a systemic granulomatous, and debilitating disease. Focus is given on cell wall carbohydrate and protein contents, their immune-stimulatory features, adhesion properties, drug target characteristics, and morphological phase specificity. We offer a journey toward the future understanding of the dynamic nature of the cell wall and of the changes that may occur when the fungus infects the human host.

show abstract

Isolation and wall analysis of dimorphic mutants ofParacoccidioides brasiliensis

Cited by 25 publications

References 13 publications

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Leukotrienes are involved in leukocyte recruitment induced by live Histoplasma capsulatum or by the β‐glucan present in their cell wall

Plasma membrane lipids and their role in fungal virulence

The Paracoccidioides Cell Wall: Past and Present Layers Toward Understanding Interaction with the Host

Contact Info

Product

Resources

About