Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype

Abstract

It appears that cellular functions such as signalling, sugar and ion transport and transcript stability play an important role in conferring higher flooding tolerance in the FR13A rice type.

Anoxia-enhanced expression of genes isolated by suppression subtractive hybridization from pondweed (Potamogeton distinctus A. Benn.) turions

“…However, some important genes whose expression is speciWc to anoxia were reported in this study. Most of these genes had not been found in studies on submerged rice (Agarwal and Grover 2005) and submerged Sesbania rostrata (Caturla et al 2002) done by similar methods.…”

“…The third group contained three proteins related to transport; vacuolar H + -translocating pyrophosphatase (V-PPase), a cation transporter-like protein and a plasma membrane intrinsic protein (PIP). V-PPase was Wrst reported to be induced in rice seedlings under anoxia (Carystinos et al 1995), and cDNA fragments encoding V-PPases were also isolated in a study on Xooding-induced genes in rice by a subtraction method (Agarwal and Grover 2005). A cation transporter-like protein is thought to be a homologue of OsCTP, which is induced by submergence in rice and structurally similar to cation transport protein ChaC in Escherichia coli (Qi et al 2005).…”

“…In the case of anoxic Arabidopsis, more than 600 out of over 22,500 genes were predicted by Loreti et al (2005) to be induced. From rice, the most tolerant plant species to anoxia among major crops, many genes up-regulated by Xooding stress have been isolated by subtractive hybridization methods (Agarwal and Grover 2005;Qi et al 2005;Sauter et al 2002). Caturla et al (2002) used suppression subtractive hybridization to isolate genes enhanced by submergence in adventitious root primordial of Sesbania rostrata.…”

Evaluation of Metabolite Alteration under Flooding Stress in Soybeans

“…However, the influence of flooding stress on metabolites related to fermentation and glycolysis remains unclear, although the expression of these proteins was affected by flooding stress. Recently, transcriptome studies revealed that hypoxia and flooding stress affected the expression of genes related to carbon metabolism, nitrogen metabolism, cell wall formation, and signal transduction, as well as transcription factors 1,19,23,39 . These reports suggested that many metabolites are affected by flooding stress, making it necessary to elucidate the changes in numerous metabolites using a metabolomics technique which may enable a comprehensive understanding of plant response to flooding.…”

Submergence-inducible and circadian rhythmic basic helix–loop–helix protein gene in Nicotiana tabacum

“…Flooding dramatically affects biochemical processes in plants, most significantly due to slow diffusion of oxygen and carbon dioxide, and stimulates a variety of responses, such as anaerobic fermentation, restriction in shoot elongation and carbohydrate consumption, and aerenchyma formation, which can enable the survival of the plants (Armstrong and Drew, 2002;Voesenek et al, 2006;Xu et al, 2006;Perata and Voesenek, 2007;Septiningsih et al, 2008). In relation to the flooding stress in plants, much of the research effort has been focused on the glycolysis and fermentation pathways (Dennis et al, 2000;Voesenek et al, 2006), and it has become increasingly evident that there are diverse and complex components including transcription factors involved in the flooding-response in plants (Klok et al, 2002;Agarwal and Grover, 2005).…”