Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran

Hayati, Masoumeh; Shamseddini, Mehrdad; Tahamtan, Yahya; Sadeghzadeh, Safar; Manavian, Mohsen; Nikoo, Davood

doi:10.34172/ijep.2020.20

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Kumar et al (2014) detected C. perfringens DNA in 59.62% of sheep isolates by PCR while Nazkiet al (2017) showed that 70.62% of isolates were positive for C. perfringens also . Hayati and Tahamtan (2021) noted that out of 167 suspected cases, 61% were positive for C.perfringens.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of Clostridium perfringens in small ruminants

Mohamed,

Elfeky,

Abd Al-Azeem

et al. 2023

SVU-International Journal of Veterinary Sciences

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A significant foodborne pathogen as Clostridium perfringens (C. perfringens) has been linked to many diseases in sheep and goat, included; Enterotoxaemia, pulpy kidney disease,struck, and even animal mortality. So, The objective of this study is to discuss the presence of C. perfringens in small ruminants with focusing on the virulence factors, antimicrobial resistance profile, and biofilmforming capacities of the organism. A total 166 samples, (95 from sheep, 71 from goats) collected from apparently healthy, diarrheic animals and dead carcasses suspected to be infected with enterotoxaemia in Sohag, Egypt. These samples were subjected for Animal health research institute lab for microbiological examination. The phenotypic identification revealed that 25 isolates (15.1%) were Clostridium spp. 10 isolates were identified as C. perfringens by PCR using a species-specific 16S rRNA gene. The results of multiplex PCR, revealed that the cpa gene, which is responsible for C. perfringens toxintype A, had the highest prevalence (90%) followed by the cpe gene, which is responsible for type F, while none of the isolates had the cpb, etx, and iap genes. Phenotypically, the majority of the isolates displayed multidrug resistance (MDR) patterns for, vancomycin, tetracycline , oxacillin, and piperacillin,. erythromycin and ceftazidime and whereas genetically, our isolates had the bla(70%), ermB (40%) and tetK (30%) genes. Our isolates demonstrated a moderate (10%) and weak (60%) ability to form biofilm.

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Section: Resultsmentioning

confidence: 99%

Molecular characterization of Clostridium perfringens in small ruminants

Mohamed,

Elfeky,

Abd Al-Azeem

et al. 2023

SVU-International Journal of Veterinary Sciences

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“…PCR displayed that type A was the most prevalent toxin in all examined animals followed by D and B as shown in Figure 1D . Many reports confirmed that finding of type A of CP was the highly detected type in cattle and sheep samples ( Elsify et al ., 2016 ; Hosseinzadeh et al ., 2018 ; Hayati et al ., 2020 ) . Moreover, Nazki et al .…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera as a potential antimicrobial against pathogenic Clostridium perfringens isolates in farm animals

Ibrahim,

Altammar

2024

Open Vet J

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Background: Clostridium perfringens (Cp) is an emerging anaerobic pathogen that can aggravate sever fatal infections in different hosts and livestock. Aim: This paper was designed to monitor the antibacterial efficacy of Moringa oleifera (M. oleifera) plant against different CP isolates of variant toxin genotypes comparing that with commercial antibiotics in veterinary field. Methods: Total of 200 examined fecal, intestinal and liver samples from cattle, sheep and goats were investigated bacteriologically and biochemically for C. perfringens. Then the isolates were examined by polymerase chain reaction (PCR) for toxin genes typing. Thereafter, the antimicrobial susceptibility testing as well as the antibacterial efficacy of M. oleifera were evaluated and statistically analyzed against recovered isolates. Results: The prevalence rate of CP was 51% (102/200); of which 54.5% was from cattle, 50% from sheep and 40% from goat. Moreover, all CP isolates were highly resistant to tetracycline and lincomycin drugs; meanwhile they were of the least resistance against ciprofloxacin (8.3%-16.7%), cefotaxime (16.7%-25%) and gentamycin (26.7%-33.3%). For M. oleifera, high antibacterial efficacy with greater inhibition zones of the plant was recorded with its oil (20-24 mm) and ethanolic extracts (16-20 mm) against C. perfringens than the aqueous extract (≤10 mm). A good correlation was stated between M. oleifera oil and toxin type of C. perfringens isolates particularly type A followed by D and B types. Interestingly, the oil and ethanolic extracts of M. oleifera gave higher antibacterial efficacy than most commercial antibiotics against the recovered isolates. Conclusion: This study highlighted the potent antibacterial properties of M. oleifera for suppressing C. perfringens isolated from farm animals; hence, more investigations on M. oleifera are suggested to support its use as a medical herbal plant substituting antibiotics hazards and resistance problems worldwide.

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