Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp

Sudjaroen, Yuttana; Haubner, Roswitha; Würtele, Gerd; Hull, William E.; Erben, Gerhard; Spiegelhalder, B; Changbumrung, Supranee; Bartsch, Helmut; Owen, Robert W.

doi:10.1016/j.fct.2005.05.013

Cited by 212 publications

(166 citation statements)

References 22 publications

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“…This suggests that P. sidoides should also show anticancer activity. Mass spectrometry analysis revealed that the PST was rich in sulphated coumarins and flavonoids that were identified based on the loss of 80 mass units to yield major fragments of 193 m/z for dihydroxycoumarin (Gödecke et al 2005), 303 m/z for gallocatechin (GC)/epigallocatechin (EGC) and 287 m/z for eriodictyol (Callemien & Collin 2008;Sudjaroen et al 2005). Sulphated coumarins were also found by Latté et al (2000) and Gödecke et al (2005) in extracts prepared with 80% acetone from the dried roots and aerial parts of P. sidoides, respectively.…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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Context Pelargonium sidoides DC (Geraniaceae) is an important medicinal plant indigenous to South Africa and Lesotho. Previous studies have shown that root extracts are rich in polyphenolic compounds with antibacterial, antiviral and immunomodulatory activities. Little is known regarding the anticancer properties of Pelargonium sidoides extracts. Objective This study evaluates the anti-proliferative effects of a Pelargonium sidoides radix mother tincture (PST). Materials and methods The PST was characterized by LC-MS/MS. Anti-proliferative activity was evaluated in the pre-screen panel of the National Cancer Institute (NCI-H460, MCF-7 and SF-268) and the Jurkat leukaemia cell line at concentrations of 0-150 mg/mL. The effect on cell growth was determined with sulphorhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays after 72 h. The effect on cell cycle and apoptosis induction in Jurkat cells was determined by flow cytometry with propidium iodide and Annexin V: fluorescein isothiocyanate staining. Results Dihydroxycoumarin sulphates, gallic acid as well as gallocatechin dimers and trimers were characterized in PST by mass spectrometry. Moderate anti-proliferative effects with GI 50 values between 40 and 80 mg/mL were observed in the NCI-pre-screen panel. Strong activity observed with Jurkat cells with a GI 50 value of 6.2 mg/mL, significantly better than positive control 5-fluorouracil (GI 50 value of 9.7 mg/mL). The PST arrested Jurkat cells at the G 0 /G 1 phase of the cell cycle and increased the apoptotic cells from 9% to 21%, while the dead cells increased from 4% to 17%. Conclusion We present evidence that P. sidoides has cancer cell type-specific anti-proliferative effects and may be a source of novel anticancer molecules. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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“…The light absorption of the dye solution from sweet tamarind seed was lower than that of the sour tamarind seed, indicating a less color strength and/or larger amount of water-soluble impurities present in the solution prepared from the TO dye. The condensed tannins which are a polyphenolic compound was reported to be presented in the solution of the tamarind-seed dye [2], [10]. …”

Section: A Composition Of the Tamarind Seedsmentioning

confidence: 99%

Dyeing of Cotton, Bombyx Mori and Eri Silk Fabrics with the Natural Dye Extracted from Tamarind Seed

Tepparin¹,

Sae-be²,

Suesat³

et al. 2012

IJBBB

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Abstract-This research studied the extraction of the natural dye from two different types of tamarind seed viz. sour-tamarind seed (TF) and sweet-tamarind seed (TO). The extracted dye solutions were prepared into a powder form and subsequently used for dyeing cotton and Bombyx Mori silk and Eri silk fabrics. From the experiment, the TF dye powder was easier soluble in water and could render a higher color strength than the TO dye powder. It was found for the tamarind-seed dyes that they could better exhausted on the silk fabrics than the cotton. Between the two silk fabrics, the dyes illustrated a higher degree of exhaustion on Bombyx Mori silk fabric than the Eri counterpart. The tamarind-seed dyes provide a reddish brown shade on the fabrics. This reddish brown shade could be altered when the dyed fabrics were put through the washing process. After washing, the shade of the dyed fabrics was more intense. The color fastness to washing of the dyed fabrics was in a good-excellent level. Even after experiencing 5-time washing, no significant shade change was observed on the dyed fabrics. The effect of mordants on the dyed fabrics was studied using three different mordants which were ZnSo 4 , K 2 Cr 2 O 7 and FeSo 4 . The ZnSo 4 mordant was found to enhance the color fastness to washing to the dyed fabrics without causing any shade change, while using K 2 Cr 2 O 7 as a mordant led to a shade alteration into a yellow brown but helped to stabilize the dye molecule, so such shade obtained was not sensitive to the washing condition. For the FeSo 4 mordant, it also caused the shade change on the dyed fabrics to a dull grey shade but providing an excellent color fastness to washing.

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“…The ability of antioxidants to destroy highly reactive free radicals serves to protect the structural integrity of immune cells and prevents the loss of essential functions. This study proposes that tamarind seed flavonoids might contain bioactive components which act as antioxidant responsible for neutralizing the effect of free radicals which are normally generated by Oxygen-dependent mechanisms for intracellular killing by leukocytes as reported earlier (Sudjaroen et al, 2005). PE exhibits the fastest rate of healing that contains highest protein content.…”

Section: Discussionmentioning

confidence: 99%

Tamarind Seed Extract Enhances Epidermal Wound Healing

Mohamad¹,

Akram²,

Bero³

et al. 2011

IJB

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Traditional healing power of tamarind fruits and the established antioxidant activity of the seeds drive the present study. Wound healing efficiency of tamarind seed was evaluated. Different solvents: phosphate buffer saline (PBS), water, methanol and ethanol were used to prepare the extract. Circular wound was inflicted on the nape. 10 µl of 5 µg/ml of extract was applied topically twice daily. Wound area was measured using Adobe ®Photoshop C3 Extended version and the percentage of wound reduction was calculated. PBS extract treatment induced complete wound healing in shortest period (10 days) while water extract, methanol extract and Solcoseryl ointment treatment induced complete wound healing in 11 days and control groups without any treatment took 14 days to heal. Phytochemical screening and Bradford method for protein quantification reveals the presence of alkaloid, saponin and tannin in all samples except PBS extract which tested negative to tannin. Flavonoid tested positive in methanol and ethanol extracts.

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Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp

Cited by 212 publications

References 22 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Dyeing of Cotton, Bombyx Mori and Eri Silk Fabrics with the Natural Dye Extracted from Tamarind Seed

Tamarind Seed Extract Enhances Epidermal Wound Healing

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Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp

Cited by 212 publications

References 22 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Dyeing of Cotton, Bombyx Mori and Eri Silk Fabrics with the Natural Dye Extracted from Tamarind Seed

Tamarind Seed Extract Enhances Epidermal Wound Healing

Contact Info

Product

Resources

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells