Isolation and Structural Elucidation of Antioxidant Peptides from Oyster (Saccostrea cucullata) Protein Hydrolysate

Abstract

Protein derived from the oyster (Saccostrea cucullata) was hydrolyzed using protease from Bacillus cereus SU12 for isolation of antioxidant peptides. The oyster hydrolysate exhibited a strong antioxidant potential in DPPH (85.7±0.37%) followed by Hydrogen peroxide radical scavenging activity (81.6±0.3%), Hydroxyl radical-scavenging activity (79.32±0.6%), Reducing power assay (2.63±0.2 OD at 700nm). Due to the high antioxidant potential, hydrolysate was fractionated in Sephadex G-25 gel filtration chromatography. The active peptide fraction was further purified by UPLC-MS. Totally 7 antioxidant peptides were collected. Among 7 peptides (SCAP 1-7), 3 peptides (SCAP 1, 3 and 7) had highest scavenging ability on DPPH radicals. The amino acid sequence and molecular mass of purified antioxidant peptides (SCAP1, SCAP3 and SCAP7) were determined by Q-TOF ESI mass spectroscopy and structures of the peptides were Leu-Ala-Asn-Ala-Lys (MW=515.29Da), Pro-Ser-Leu-Val-Gly-Arg-Pro-Pro-Val-Gly-Lys-Leu-Thr-Leu (MW=1432.89Da) and Val-Lys-Val-Leu-Leu-Glu-His-Pro-Val-Leu (MW=1145.75Da), respectively. The unique amino acid composition and sequence in the peptides might play an important role in expression of their antioxidant activity. The results of this study suggest that oyster protein hydrolysate is good source of natural antioxidants.

Amelioration of Cyclophosphamide-Induced Hepatotoxicity by the Brown Seaweed Turbenaria ornata

“…The inhibition in lipid peroxidation may be due to the presence of polyphenolic antioxidants that were reported to disrupt free-radical chain reaction by donating proton to fatty acid radicals to terminate chain reactions, may have roles to inhibit lipid peroxidation [57]. Phenolic compounds are considered to exhibit radical scavenging properties [58]. Several studies demonstrated a significant correlation between the phenolic content and the antioxidant activity in seaweed extracts [59].…”

Chemical and biological studies on the soft coralNephtheasp.

“…40,41 Nevertheless, a few reports exist in the literature on the synthesis of biogenic AgNPs using marine invertebrates, which have been mostly studied for their antimicrobial potential, exemplied by oyster (Saccostrea cucullata Born. ), 42 marine polychaete, 23 and sponges, e.g. Acanthella elongate Dendy, 43,44 Callyspongia diffusa Ridley, 45 Haliclona spp., 46,47 Axinella sinoxia Alvarez & Hooper (Hamed et al, 2017), 48 and Amphimedon sp.…”

Ameliorative Effects of Peptides from the Oyster (Crassostrea hongkongensis) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice

“…Evidence demonstrated that UVB irradiation could decrease the activities of antioxidant enzymes, such as SOD and GPH-Px [29], and promote oxidative damage in the skin [7]. In a previous study, peptides from oyster (Saccostrea cucullata) protein hydrolysate was reported to possess good antioxidant properties [33], indicating OPs have potential for the prevention of photoaging. Here, we demonstrated that after 10 weeks of UVB exposure, mouse dorsal skin displayed a notable decrease in activities of antioxidant enzymes.…”