Chemical and biological studies on the soft coral<i>Nephthea</i>sp.

Abdelhafez, Omnia Hesham; Fahim, John Refaat; Masri, Ramy R. El; Salem, M. Alaraby; Desoukey, Samar Yehia; Ahmed, Safwat A.; Kamel, Mohamed S.; Pimentel‐Elardo, Sheila Marie; Nodwell, Justin R.; Abdelmohsen, Usama Ramadan

doi:10.1039/d1ra03045k

Cited by 8 publications

(6 citation statements)

References 64 publications

(125 reference statements)

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“…3,7,27,28 The use of natural reducing agents derived from plants or microorganisms, along with green chemistry principles, provides a promising solution for protecting the environment while advancing technology. 29,30 AgNPs are commonly utilized as signal enhancers, optical receptors, intercalating materials of batteries, polarizing lters, catalysts, sensors, bio-labelling materials, anticancer, antioxidants, and antimicrobial agents. In the ght against cancer, AgNPs have been shown to reduce toxicity, enhance surface plasmon resonance, and expand electrical resistance.…”

Section: Introductionmentioning

confidence: 99%

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Phytofabrication of silver nanoparticles using Averrhoa bilimbi leaf extract for anticancer activity

2023

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytofabrication of silver nanoparticles using Averrhoa bilimbi leaf extract for anticancer activity

2023

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“…Additionally, they have numerous unique scaffolds, which afford more and more inspiration for drug discovery [16][17][18][19][20][21][22][23][24][25]. Though terrestrial plants as well as bacteria have usually been the main resources of natural metabolites, in the last 30 years, marine ecosystems, including their invertebrates, plants, as well as bacteria, have also been the main sources of numerous novel structural metabolites, which are identified as natural marine products [26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Pro-Apoptotic Activity of Epi-Obtusane against Cervical Cancer: Nano Formulation, In Silico Molecular Docking, and Pharmacological Network Analysis

Abdelhafez,

Abdel-Rahman,

Alaaeldin

et al. 2023

Pharmaceuticals

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Cancer is a major disease that threatens human health all over the world. Intervention and prevention in premalignant processes are successful ways to prevent cancer from striking. On the other hand, the marine ecosystem is a treasure storehouse of promising bioactive metabolites. The use of such marine products can be optimized by selecting a suitable nanocarrier. Therefore, epi-obtusane, previously isolated from Aplysia oculifera, was investigated for its potential anticancer effects toward cervical cancer through a series of in vitro assays in HeLa cells using the MTT assay method. Additionally, the sesquiterpene was encapsulated within a liposomal formulation (size = 130.8 ± 50.3, PDI = 0.462, zeta potential −12.3 ± 2.3), and the antiproliferative potential of epi-obtusane was investigated against the human cervical cancer cell line HeLa before and after encapsulation with liposomes. Epi-obtusane exhibited a potent effect against the HeLa cell line, while the formulated molecule with liposomes increased the in vitro antiproliferative activity. Additionally, cell cycle arrest analysis, as well as the apoptosis assay, performed via FITC-Annexin-V/propidium iodide double staining (flow cytofluorimetry), were carried out. The pharmacological network enabled us to deliver further insights into the mechanism of epi-obtusane, suggesting that STAT3 might be targeted by the compound. Moreover, molecular docking showed a comparable binding score of the isolated compound towards the STAT3 SH2 domain. The targets possess an anticancer effect through the endometrial cancer pathway, regulation of DNA templated transcription, and nitric oxide synthase, as mentioned by the KEGG and ShinyGo 7.1 databases.

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“…Moreover, biological investigations carried out on Nephthea sp. soft coral demonstrated their varied potential as anticancer [12,13], anti-inflammatory [14], and antidermatophyte [15] agents.…”

Section: Introductionmentioning

confidence: 99%

Bioactive constituents of Marine Soft Coral Nephthea sp. against Herpes simplex type I (HSV-1) and Coxsackie B4 (CoxB4) viruses; In-Vitro and In Silico studies

Hassan

elhawary

Emam³

et al. 2022

Egypt. J. Chem.

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The most catastrophic problem that affects living standards is viral infections. This work aims to discover and evaluate new antiviral agents from marine sources. Antiviral activities of n-hexane, dichloromethane, ethyl acetate, and butanol fractions of octocoralNephtheasp. were examined in vitro against Herpes simplex type I (HSV-1) and Coxsackie B4 (CoxB4) viruses using MTT assay. n-hexane fraction was the most bioactive fraction with IC50 30.23 ± 0.007g/ml on both viruses. The metabolic profiling of the n-hexane fraction was investigated as the most potent fraction via the Ultra Performance Liquid Chromatography method joined to a quadrupole time-of-flight hybrid mass spectrometer (UPLC-Q/TOF-MS), which led to the identification of twelve secondary metabolites (diterpenoid, triterpene, steroids, and fatty acids). A molecular docking investigation was supported by using Molecular Operating Environment (MOE) software to prove the mechanism of action. The highest binding energy score was for lauric acid with -10.2157 kcal/mol toward the Thymidine Kinase (TK), and Chabrolohydroxybenzoquinone G toward 3C protease (3Cpro) enzyme with -16.5115 kcal/mol. Finally, the results were confirmed by the inhibitory effect of bioactive fraction in-vitro against TK and 3Cpro enzymes. Our results highlighted Nephtheasp. as a rich source of non-polar effective constituents that might be a promising candidate for controlling viral infections.

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Chemical and biological studies on the soft coralNephtheasp.

Abstract: The cytotoxic potential of the crude extract, different fractions, and green synthesized nanoparticles of the soft coral Nephthea sp. was studied, supported by LC-HR-ESI-MS metabolomics analysis and molecular docking of the dereplicated compounds.

Cited by 8 publications

References 64 publications

Phytofabrication of silver nanoparticles using Averrhoa bilimbi leaf extract for anticancer activity

Phytofabrication of silver nanoparticles using Averrhoa bilimbi leaf extract for anticancer activity

Pro-Apoptotic Activity of Epi-Obtusane against Cervical Cancer: Nano Formulation, In Silico Molecular Docking, and Pharmacological Network Analysis

Bioactive constituents of Marine Soft Coral Nephthea sp. against Herpes simplex type I (HSV-1) and Coxsackie B4 (CoxB4) viruses; In-Vitro and In Silico studies

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