Isolation and Structural Characterization of the Most Active Antidiabetic Fraction of Corchorus olitorius Seed Extract

Pharmacognostic Study of a Plant Seed Extract

“…The following description of the process of seed preparation, extraction, fractionation, and bioassay is guided by works of Egua et al [10,[25][26][27]. Care is taken here at the generalization of the procedure for all seeds and plant parts and advice for an educated modification in choice of solvents for intending users.…”

“…The column fractions are eluted using hexane, hexane ethyl acetate, ethyl acetate, ethyl acetate methanol, and methanol (in order of polarity) as mobile phase. The obtained pure compounds are isolated and identified through thin layer chromatography (TLC) and functional group analysis by nuclear magnetic resonance (NMR) spectroscopy [26,27].…”

“…The column fractions are further eluted using hexane ethyl acetate (i.e., 99-1%…1-99%), ethyl acetate, (in order of polarity) as mobile phase. This was done by increasing the polarity by 1% alteration in eluent solvents' ratio (i.e., ethyl acetate 100%; ethyl acetate 99%/methanol 1%; till methanol 100%) [27]. The process is guided by thin layer chromatography (TLC) monitoring, for an effective separation in the eluent (solution containing "pure compound").…”

“…The choice for these three models was influenced by fund, availability, technical skill, and satisfaction of study requirement. At the stage of bioassaying (testing) the pure compound, the only model used was the definitive alloxan-induced DM [27].…”

“…After a successful bioassay of the pure compounds, that (pure compound) with the best activity is sent for structural elucidation through functional group analysis by nuclear magnetic resonance (NMR) spectroscopy and confirmed with gas chromatography and mass spectroscopy (GC MS) [27]. The readings are then read out by an experienced chemist.…”