Isolation and species identification of enterococci from clinical specimen with their antimicrobial susceptibility pattern in a tertiary care hospital, Bangladesh

Antimicrobial Susceptibility Pattern of Enterococci Isolated from Clinical Specimens at Mymensingh Medical College Hospital, Mymensingh, Bangladesh

“…Enterococci were identified by colony morphology, Gram staining, absence of catalase production, tolerance to 6.5% NaCl, growth on bile esculin agar with esculin hydrolysis [1]. Species identification was done by PCR was done to detect E. faecalis and E. faecium by using species specific primers [8].…”

“…Standard disc diffusion techniques as recommended by the Clinical and Laboratory Standard Institute were performed for susceptibility testing of Ampicillin, Gentamicin, Nitrofurantoin, Imipenem, Vancomycin, Ciprofloxacillin, Ceftriaxone, Cefuroxime, Cotrimoxazole, Linezolid. For molecular study, DNA was extracted by heat mehod 100˚C for 10 mintues [1]. Multiplex PCR was performed by using species specific primers for Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium), vanA, vanB, vanC1, vanC2/C3 genes for vancomycin resistance [8].…”

“…Enterococci are the member of normal flora in the gut of humans and animals [1]. Enterococci have been recognized as being potentially pathogenic for humans since the turn of the century [2].…”

“…Enterococci have been recognized as being potentially pathogenic for humans since the turn of the century [2]. Though they are not considered to be highly virulent, their intrinsic resistance and ability to acquire resistance to several broad spectrum antibiotics allow them to cause super infections in patients already receiving antimicrobial therapy [1]. In the last decade, enterococci have become the surgical wound infections and nosocomial urinary tract infections and the third most frequently reported cause of bacteraemia in humans [3].…”

“…Species identification is useful for epidemiological investigation of an outbreak and also for clinical decisions, particularly with regard to therapy, as antimicrobial susceptibility differs by species [6]. Biochemical tests for species identification are not performed routinely as they are laborious and time consuming; so to overcome the problem, the use of molecular methods has been suggested [1].…”