Commensal Lactobacilli Metabolically Contribute to Cervical Epithelial Homeostasis in a Species-Specific Manner

Jimenez, Nicole R.; Maarsingh, Jason D.; Łaniewski, Paweł; Herbst-Kralovetz, Melissa M.

doi:10.1128/msphere.00452-22

Cited by 6 publications

(6 citation statements)

References 131 publications

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“…1.7 Other metaboliteshydrogen peroxide, bacteriocins, biosurfactant, sialic acid and sialidase activity Beyond lactic acid and SCFAs, beneficial bacteria produce additional metabolites such as hydrogen peroxide, bacteriocins, and biosurfactants, all of which contribute substantially to maintain the health and homeostasis of the cervicovaginal environment (95).…”

Section: Cervicovaginal Microbiota Instigated Immune Modulation On Sptbmentioning

confidence: 99%

“…It is important to note that the causality of sPTB cannot be solely attributed to the presence or absence of specific bacteria. The composition of the cervicovaginal microbiota and the diversity of microbial species within it, plays a significant role in shaping the metabolite profile within the cervicovaginal environment ( 13 , 60 , 95 ) owing to the inherent metabolic capability of microbiota ( 96 ). These metabolites can influence cervicovaginal homeostasis ( 60 , 95 ), yet the precise mechanisms by which their interaction impacts sPTB remain elusive.…”

Section: Introductionmentioning

confidence: 99%

“…The composition of the cervicovaginal microbiota and the diversity of microbial species within it, plays a significant role in shaping the metabolite profile within the cervicovaginal environment ( 13 , 60 , 95 ) owing to the inherent metabolic capability of microbiota ( 96 ). These metabolites can influence cervicovaginal homeostasis ( 60 , 95 ), yet the precise mechanisms by which their interaction impacts sPTB remain elusive. The dynamic metabolic ecosystem within the cervicovaginal environment encompasses diverse pathways involving carbohydrates and amino acids, contributing to its complex nature, believed to influence pregnancy outcomes.…”

Section: Introductionmentioning

confidence: 99%

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Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Onyango,

Mi,

Koech

et al. 2023

Front. Immunol.

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Differences in the cervicovaginal microbiota are associated with spontaneous preterm birth (sPTB), a significant cause of infant morbidity and mortality. Although establishing a direct causal link between cervicovaginal microbiota and sPTB remains challenging, recent advancements in sequencing technologies have facilitated the identification of microbial markers potentially linked to sPTB. Despite variations in findings, a recurring observation suggests that sPTB is associated with a more diverse and less stable vaginal microbiota across pregnancy trimesters. It is hypothesized that sPTB risk is likely to be modified via an intricate host-microbe interactions rather than due to the presence of a single microbial taxon or broad community state. Nonetheless, lactobacilli dominance is generally associated with term outcomes and contributes to a healthy vaginal environment through the production of lactic acid/maintenance of a low pH that excludes other pathogenic microorganisms. Additionally, the innate immunity of the host and metabolic interactions between cervicovaginal microbiota, such as the production of bacteriocins and the use of proteolytic enzymes, exerts a profound influence on microbial populations, activities, and host immune responses. These interplays collectively impact pregnancy outcomes. This review aims to summarize the complexity of cervicovaginal environment and microbiota dynamics, and associations with bacterial vaginosis and sPTB. There is also consideration on how probiotics may mitigate the risk of sPTB and bacterial vaginosis.

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Section: Cervicovaginal Microbiota Instigated Immune Modulation On Sptbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Onyango,

Mi,

Koech

et al. 2023

Front. Immunol.

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“…132 It has been increasingly popular in the last few years for the functional characterization of microbial communities based on capturing the physical effectors (i.e., low-molecular-weight products of gene expression called metabolites) of any metabolic activity or within-microbiota and host-microbiota interaction. [133][134][135] In the field of human reproduction, meta-metabolomics has been mainly applied to studies of the vaginal tract 136,137 Box 4 -Meta-proteomics…”

Section: Meta-metabolomicsmentioning

confidence: 99%

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

Salas-Espejo,

Terrón-Camero,

Ruiz

et al. 2023

Semin Reprod Med

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Microorganisms are important due to their widespread presence and multifaceted roles across various domains of life, ecology, and industries. In humans, they underlie the proper functioning of multiple systems crucial to well-being, including immunological and metabolic functions. Emerging research addressing the presence and roles of microorganisms within human reproduction is increasingly relevant. Studies implementing new methodologies (e.g., to investigate vaginal, uterine, and semen microenvironments) can now provide relevant insights into fertility, reproductive health, or pregnancy outcomes. In that sense, cutting-edge sequencing techniques, as well as others such as meta-metabolomics, culturomics, and meta-proteomics, are becoming more popular and accessible worldwide, allowing the characterization of microbiomes at unprecedented resolution. However, they frequently involve rather complex laboratory protocols and bioinformatics analyses, for which researchers may lack the required expertise. A suitable pipeline would successfully enable both taxonomic classification and functional profiling of the microbiome, providing easy-to-understand biological interpretations. However, the selection of an appropriate methodology would be crucial, as it directly impacts the reproducibility, accuracy, and quality of the results and observations. This review focuses on the different current microbiome-related techniques in the context of human reproduction, encompassing niches like vagina, endometrium, and seminal fluid. The most standard and reliable methods are 16S rRNA gene sequencing, metagenomics, and meta-transcriptomics, together with complementary approaches including meta-proteomics, meta-metabolomics, and culturomics. Finally, we also offer case examples and general recommendations about the most appropriate methods and workflows and discuss strengths and shortcomings for each technique.

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“…18 Recently, we discovered that commensal vaginal Lactobacillus species, including Lactobacillus crispatus, Lactobacillus paragasseri, and Lactobacillus iners, were capable of producing PLA in vitro. 19,20 Within the lower female reproductive tract, it is well established that microbiota dominated by Lactobacillus spp. provide protection against pathogenic diseases, such as sexually transmitted infections, 21,22 urinary tract infections, 23 and bacterial vaginosis.…”

Section: Introductionmentioning

confidence: 99%

Development of phenyllactic acid ionic liquids and evaluation of cytotoxicity to human cervical epithelial cells

Crossley,

Sutar,

Tsoy

et al. 2024

RSC Adv.

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Commensal Lactobacilli Metabolically Contribute to Cervical Epithelial Homeostasis in a Species-Specific Manner

Abstract: Lactobacillus species contribute to cervicovaginal health by their production of lactic acid and other antimicrobial compounds. Yet, much is still unknown regarding the metabolic potential of lesser-studied but common vaginal lactobacilli.

Cited by 6 publications

References 131 publications

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Microbiota dynamics, metabolic and immune interactions in the cervicovaginal environment and their role in spontaneous preterm birth

Exploring the Microbiome in Human Reproductive Tract: High-Throughput Methods for the Taxonomic Characterization of Microorganisms

Development of phenyllactic acid ionic liquids and evaluation of cytotoxicity to human cervical epithelial cells

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