Isolation and sequence of rat testis cdna for a calcium binding polypeptide similar to the regulatory subunit of calcineurin

Sugimoto, Mikio; Matsui, Hideki; Etoh, S; Shimizu, Tohru; Nishio, Hisahide; Moia, Lizomar J.M.P.; Tokuda, Masaaki; Itano, Toshifumi; Takenaka, Ikumasa; Hatase, Osamu

doi:10.1016/s0006-291x(05)81362-2

Cited by 10 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are two major isoforms (Aα, Aβ) of calcineurin A (Giri et al 1991). Calcineurin B also has a testis-specific isoform (Mukai et al 1991;Sugimoto et al 1991). Calcineurin B also has a testis-specific isoform (Mukai et al 1991;Sugimoto et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of calcineurin and FKBP12, the FK506-binding protein, in Hassall's corpuscles of human thymus and epidermis

Nishio

Tsuji

Tamura

et al. 2000

Histochem Cell Biol

Self Cite

View full text Add to dashboard Cite

Calcineurin, a Ca 2+ /calmodulin-dependent protein phosphatase, plays an important role in various physiological functions including T cell activation. This enzyme is a target molecule for the immunosuppressants, cyclosporin A and FK506. In the present study, we investigated immunohistochemical localization of calcineurin and FKBP12, an FK506-binding protein, in human thymus and epidermis. The catalytic subunit (calcineurin A) of calcineurin was abundantly expressed in Hassall's corpuscles which were localized in the thymic medulla and represented the terminal stages of thymic medullary epithelium. The regulatory subunit (calcineurin B) of calcineurin was also expressed in high amounts in Hassall's corpuscles. In the epidermis, which shows similarities to Hassall's corpuscles, both subunits were also abundantly expressed, and their expression increased with the differentiation of keratinocytes. FKBP12 was observed to be expressed abundantly, both in Hassall's corpuscles and the entire epidermis. These findings suggest that the differentiated forms of the two cell types, which are the thymic medullary epithelial cell and the keratinocyte, are the target for pharmacological actions of FK506.

show abstract