Isolation and Screening of Potassium and Zinc Solubilizing Bacteria  from Different Rhizosphere Soil

Dhaked, Bhagwan Singh; Triveni, S.; Reddy, R. Subhash; Padmaja, G.

doi:10.20546/ijcmas.2017.608.154

Cited by 30 publications

(9 citation statements)

References 6 publications

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“…So, application of zinc mobilizing bacteria as a bioinoculant can transform the unavailable fractions of zinc into the available one. Since, studies on screening of zinc mobilizing bacteria have been widely studied 29–32 . But, there is lack of research related to isolation and characterization of the zinc mobilizing bacteria from cow dung with reference to nutrient solubilization and plant growth associated traits.…”

Section: Discussionmentioning

confidence: 99%

Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.

Bhatt

Maheshwari

2019

Sci Rep

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Zinc is one of the micronutrients, required by all types of crops. About 10–100ppm of zinc is present in soil which is generally immobile. The cow dung sustains all life and being practice since aeons. Exploitation of cow dung bacteria can mobilize nutrients besides contributing in sustainable agriculture. Therefore, to examine mobilization of Zn, cow dung is used as a source of bacteria. The objectives of the present study were to isolate an array of bacteria from cow dung and to characterize them for their Zn (ZnO and ZnCO3) mobilization ability in addition to establish the optimum conditions for dissolution of zinc. A total of seventy bacterial isolates have been screened for Zn mobilization. Out of which most potent (CDK15 and CDK25) were selected to study the effect of various parameters viz. pH, temperature and concentration of Zn. These parameters were assessed qualitatively in diverse growth medium and quantitatively using Atomic absorption spectroscopy. Optimum pH and temperature for mobilization was recorded at pH 5 (ZnO) and 37 °C (ZnCO3) by CDK25, whereas, optimum zinc concentration for mobilization was recorded at 0.05% (ZnO) by CDK15. Maximum amount of Zn solubilized was recorded by CDK25 in ZnO (20ppm). Considering the abilities of most potent bacterial isolates with reference to P-mobilization and growth promoting traits, pot culture assay of C. annuum L. was carried out. The findings of which conclude that, bacterium CDK25 (Bacillus megaterium) could be exploited for factors viz. nutrient management of Zn, growth promoting agent, and Zn augmentation in soil.

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Section: Discussionmentioning

confidence: 99%

Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.

Bhatt

Maheshwari

2019

Sci Rep

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“…After incubation at 30˚C for 7 days, plates were observed for halo-zones, and then solubilization index was recorded. Rhizobacterial isolates were tested for their potassium (K) solubilisation by spot inoculation on modified Aleksandrov medium containing mica powder as an insoluble source of K [15].…”

Section: Inorganic Phosphate (Pi) and Potassium (K) Solubilization Assaymentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

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Salt stress is one of the devastating factors that hampers growth and productivity of soybean. Use of Pseudomonas pseudoalcaligenes to improve salt tolerance in soybean has not been thoroughly explored yet. Therefore, we observed the response of hydroponically grown soybean plants, inoculated with halotolerant P. pseudoalcaligenes (SRM-16) and Bacillus subtilis (SRM-3) under salt stress. In vitro testing of 44 bacterial isolates revealed that four isolates showed high salt tolerance. Among them, B. subtilis and P. pseudoalcaligenes showed ACC deaminase activity, siderophore and indole acetic acid (IAA) production and were selected for the current study. We determined that 10 6 cells/mL of B. subtilis and P. pseudoalcaligenes was sufficient to induce tolerance in soybean against salinity stress (100 mM NaCl) in hydroponics by enhancing plant biomass, relative water content and osmolytes. Upon exposure of salinity stress, P. pseudoalcaligenes inoculated soybean plants showed tolerance by the increased activities of defense related system such as ion transport, antioxidant enzymes, proline and MDA content in shoots and roots. The Na + concentration in the soybean plants was increased in the salt stress; while, bacterial priming significantly reduced the Na + concentration in the salt stressed soybean plants. However, the antagonistic results were observed for K + concentration. Additionally, soybean primed with P. pseudoalcaligenes and exposed to 100 mM NaCl showed a new protein band of 28 kDa suggesting that P. pseudoalcaligenes effectively reduced salt stress. Our results showed that salinity tolerance was more pronounced in P. pseudoalcaligenes as compared to B. subtilis. However, a detailed study at molecular level to interpret the mechanism by which P. pseudoalcaligenes alleviates salt stress in soybean plants need to be explored.

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“…Similarly, Mehmood et al (2021) also demonstrated that rhizobacteria have ability to solubilize zinc under saline conditions. Dhaked et al (2017) also screened zinc solubilizing bacteria from different rhizosphere having solubilization efficiency ranges from 333% to 150%.…”

Section: Discussionmentioning

confidence: 99%

Dominance of Bacillus species in the wheat (Triticum aestivum L.) rhizosphere and their plant growth promoting potential under salt stress conditions

Zahra

Tariq

Abdullah

et al. 2023

PeerJ

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Wheat (Triticum aestivum L.) is a major source of calorific intake in its various forms and is considered one of the most important staple foods. Improved wheat productivity can contribute substantially to addressing food security in the coming decades. Soil salinity is the most serious limiting factor in crop production and fertilizer use efficiency. In this study, 11 bacteria were isolated from wheat rhizosphere and examined for salt tolerance ability. WGT1, WGT2, WGT3, WGT6, WGT8, and WGT11 were able to tolerate NaCl salinity up to 4%. Bacterial isolates were characterized in vitro for plant growth-promoting properties including indole-3-acetic acid (IAA) production, phosphate solubilization, nitrogen fixation, zinc solubilization, biofilm formation, and cellulase-pectinase production. Six isolates, WGT1, WGT3, WGT4, WGT6, WGT8, and WGT9 showed IAA production ability ranging from 0.7–6 µg m/L. WGT8 displayed the highest IAA production. Five isolates, WGT1, WGT2, WGT5, WGT10, and WGT11, demonstrated phosphate solubilization ranging from 1.4–12.3 µg m/L. WGT2 showed the highest phosphate solubilization. Nitrogen fixation was shown by only two isolates, WGT1 and WGT8. Zinc solubilization was shown by WGT1 and WGT11 on minimal media. All isolates showed biofilm formation ability, where WGT4 exhibited maximum potential. Cellulase production ability was noticed in WGT1, WGT2, WGT4, and WGT5, while pectinase production was observed in WGT2 and WGT3. Phylogenetic identification of potential bacteria isolates confirmed their close relationship with various species of the genus Bacillus. WGT1, WGT2, and WGT3 showed the highest similarity with B. cereus, WGT6 with B. tianshenii, WGT8 with B. subtilis, and WGT11 with B. thuringiensis. Biofertilizer characteristics of salt-tolerant potential rhizospheric bacteria were evaluated by inoculating wheat plants under controlled conditions and field experiments. B. cereus WGT1 and B. thuringiensis WGT11 displayed the maximum potential to increase plant growth parameters and enhance grain yield by 37% and 31%, respectively. Potential bacteria of this study can tolerate salt stress, have the ability to produce plant growth promoting substances under salt stress and contribute significantly to enhance wheat grain yield. These bacterial isolates have the potential to be used as biofertilizers for improved wheat production under salinity conditions and contribute to the sustainable agriculture.

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Isolation and Screening of Potassium and Zinc Solubilizing Bacteria from Different Rhizosphere Soil

Cited by 30 publications

References 6 publications

Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.

Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L.

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

Dominance of Bacillus species in the wheat (Triticum aestivum L.) rhizosphere and their plant growth promoting potential under salt stress conditions

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