Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production

Abstract

Nineteen fungal strains were isolated from a chicken slaughterhouse effluent and within those, only one showed high values of lipolytic activity in submerged cultures. This fungus was identified as Trametes hirsuta. The crude extract was immobilized in chitosan/clay beads, with an immobilization yield of 80.9%. The analyses of the crude extract and the immobilized derivative at different temperatures, pH (s), solvents, metallic ions and storage showed that the immobilization process increased the enzyme life span. Ethyl esters were obtained in solvent free systems using chicken viscera oil and the enzyme crude extract. For effective comparison, a reaction using viscera oil and commercial lipase Novozym 435 was carried out. The result revealed 35% and 28% esters conversion in the reactions containing chicken viscera oil, using Novozym 435 and the crude extract respectively. The extract was also used in a reaction with soybean oil, traditionally used as starting substrate for biodiesel production.

Potential biotechnological capabilities of cultivable mycobiota from carwash effluents

“…However, our findings could not compare to the hydrolysis activity of filamentous fungi isolates reported by Pacheco et al. () who reported activities ranging from 89 U/ml to 361 U/ml, using various substrates. Lipase production was targeted in this study because lipase based technologies for the synthesis of novel compounds is on the increase (Andualema & Gessesse, ).…”

Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment

“…In this regard, Sharma et al (2001) reported that trace elements assist with structural components and are stabilizers of enzymes, whereas ions such as Mg 2+ , Fe 2+ , Ca 2+ , Cu 2+ , Co 2+ , Na + , K + , Mn 2+ , and Zn 2+ appear to positively influence lipase production. The effect of the salts on the properties of the proteins is perceived by the activity, conformational stability, and solubility [44]. These effects may appear due to ionic bonds at specific sites of the proteins, altering their level of hydration [45].…”

“…Other micronutrients needed to regulate these functions are potassium, considered essential for osmoregulation [47], iron, which in turn is essentially used for synthesis of heme and cytochrome [48], and calcium, considered necessary for the effective stabilization of lipases [49]. These characteristics can be associated with structural changes of the enzyme and the fact that the salts increase the dispersion of enzymatic molecules, facilitating the mass transfer during the reaction [44].…”

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

“…A blank sample was always used containing medium described in Maia et al [ 16 ] instead of enzyme solution. The molar extinction coefficient of ε = 15,000 M −1 cm −1 for pNP at 410 nm [ 17 , 18 ] was used for calculus. One enzymatic unit was defined as the amount of the enzyme that releases one mmol of pNP in one minute under the assay conditions.…”