Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment

Mendes, Danylo Bezerra; Silva, F. F. Da; Guarda, Patrícia Martins; Almeida, Alex Fernando de; Oliveira, Deyla Paula de; Morais, Paula B.; Guarda, Emerson Adriano

doi:10.1155/2019/8182425

Cited by 15 publications

(12 citation statements)

References 47 publications

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“…Out of them, there were three lipolytic isolates with the highest lipolytic activity. Some studies have been also reported that this method was used to screen both fungal and bacterial lipolytic activities (Mendes et al, 2019;Ramnath et al, 2017). Furthermore, Kouker and Jaeger (1987) stated that this method was preferrable than others due to there was not inhibition effect to the growth or change in the physiological characters of microorganisms.…”

Section: Discussionmentioning

confidence: 99%

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

Yanti

Sriwahyuni

Rayani

et al. 2019

Preprint

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The present study was investigated the potential of lipolytic fungi (molds) isolated from landfill soil in degrading Poly-β-hydroxybutyrate (PHB). Screening of PHB-degrading lipolytic molds was done in two stages, such as screening of lipolytic molds which was identified by the formation of orange fluorescent halos around the colony on rhodamine B agar medium and the degradation PHB ability test was identified by the formation of clear zone around colony on PHB emulsion medium. Characterization of isolates was done based on phenotypic characters and the identification was done by numerical-phenetic analysis. Three lipolytic mold isolates that have ability in degrading PHB bioplastic i.e isolate KC1, KE1 and KE6. These molds have asexual spore form conidia, foot cell, septate hyphae, unbranched conidiophore, and spore mass located at the apex of phialid. The identification results showed that isolate KC1 is identic to Aspergillus terreus, KE1 is identic to Aspergillus niger and KE6 is identic to Aspergillus fumigatus.

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Section: Discussionmentioning

confidence: 99%

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

Yanti

Sriwahyuni

Rayani

et al. 2019

Preprint

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“…The six selected fungal isolates (5F, 6F, 7F, 12F, 13F and 17F) were examined for lipase production after 120hrs cultivation in 100 mL liquid medium supplemented with 1% cotton seed oil as a substrate. In previously reported F. solani was isolated from decomposition of leaves in an aquatic environment, shown lipase production when utilizing Cotton oil as a carbon source in submerged condition (28) . Similar result was obtained; Fusarium solani 7F fungi produced lipase in presence of cotton seed oil in submerged fermentation.…”

Section: Quantitative Analysis Of Lipase Enzyme Under Submerges Fermementioning

confidence: 98%

“…Lipase producing fungal strains, Aspergillus, Penicillium, Trichoderma and Mucor were isolated from palm oil mill effluent composts (27) . Where Lipase producing Bacillus licheniformis and Bacillus pumilus were isolated from cotton soapstock which will used in enzymatic degradation of soapstock (28) . Lipase produced by F. solani isolated from leaves decomposed in an aquatic environment has great potential to application in biodiesel production by transesterifcation of vegetable oils, as well as food industries in the production of fatty acid esters by hydrolysis and esterifcation.…”

Section: Introductionmentioning

confidence: 99%

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

Patel¹,

Shah²

2020

IJST

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Background/Objectives: The present study was focused to exploit the indigenous strains of fungi isolated from cotton seed soapstock for the production of the extracellular lipase through submerged fermentation technique. Methods/ Statistical analysis: Cotton seed soapstock samples used in the study contains gelatinous oil richer chemical constituents. In addition, their enrichment and diluted materials were used for the isolation of lipase producing microorganisms on tributyrin agar plates. All isolates were lipase positive confirmed by a qualitative plate assay. Quantitative estimation of Lipase production activity was measured spectrophotometrically using pnitrophenyl palmitate (p-NPP) as a substrate. In order to exploit the isolated fungal strain for industrial usage, other cellulase and protease enzymes were tested by plate assay. Morphological and molecular characteristics of selected isolates were studied. Findings: From enriched soapstock samples, a total of 49 cultures were isolated among them 19 fungal isolates were screened for lipase, cellulase, and protease activity qualitatively by plate assay. Out of 19, six fungi were selected based on their lipase activity. Highly potent Fusarium solani 7F had the ability to produce 5.95 U/mL/min. crude lipase whereas Aspergillus niger 13F has 4.2 U/mL/min. after 4days incubation at 30oC. Potent fungi culture was identified by morphological, cultural, and molecular characteristics (18s rRNA gene sequence and phylogenetic analysis) revealed them as Penicillium griseofulvum 5F, Aspergillus flavus 6F, Fusarium solani 7F, Aspergillus niger 12F, Aspergillus niger 13F, and Aspergillus terreus 17F. Novelty: Fungi was the first time reported and isolated from cotton seed soapstock materials. In future studies, this enzyme will be used in the degradation of soapstock and also in the production of biosurfactant from soapstock. Keywords: Cotton seed soapstock; Fusarium solani 7F; p-nitrophenyl palmitate; Tributyrin agar plates; degradation

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“…Since inorganic nitrogen sources can be metabolized quickly, organic nitrogen sources generally supply amino acids and additional growth factors which are necessary for cell metabolism and enzyme synthesis. Therefore, both organic and inorganic sources are used for lipase production from microbial cultivation (Mendes et al, 2019). Gomes et al (2018) observed that peptone and yeast extract sources promoted high levels of lipase Cultures were carried out in Vogel medium with 1% (w/v) vegetable oils or animal fats and 0.2% (w/v) of nitrogen source, pH 6.0, 72 h, 200 rpm at 28°C.…”

Section: Effects Of Nitrogen Sources On Single Cell Oil and Catalyst mentioning

confidence: 99%

Simultaneous high nutritional single cell oil and lipase production by Candida viswanathii

Dias

Oliveira

Brasil

et al. 2021

Acta Sci Pol Technol Aliment

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Background. Omega fatty acids are a family of polyunsaturated fats associated with several health benefits. Lipases are enzymes with potential application in several food processes such as flavor and aroma, surfactants and formulations for the dairy and bakery industries. In this study, single cell oil and lipase production by Candida viswanathii CCR8137 were evaluated simultaneously from renewable carbon sources under nitrogen limitation. Materials and methods. Enzyme and single cell oil were obtained in submerged cultivations supplemented with triolein, tributyrin, corn oil, sunflower oil, canola oil and olive oil. The effects of glucose on lipid accumulation, fatty acid profile, enzyme production and cell morphology were also evaluated. Results. The highest lipid accumulation (44.5%, w/w) was obtained from triolein, whereas olive oil was the best inducer of lipase synthesis (26.8 U/mL). Nitrogen limiting cultivations were a key parameter for an organic source which showed higher lipid accumulation and enzyme production than the tested inorganic nitrogen source. Glucose was a poor inducer of lipase synthesis, though increased values of lipid accumulation were observed from this carbon source with a maximum of 63.1% (w/w). The fatty acid profile of lipids produced by C. viswanathii CCR8137 showed a high content of omega-9 fatty acid (C18:1 n-9). The addition of glucose to the culture media resulted in the synthesis of essential fatty acids: vaccenic, linolenic and eicosadienoic acids. Conclusion. Therefore, C. viswanathii CCR8137 strain can be considered as an oleaginous yeast able to accumulate high concentrations of intracellular lipids, which are potential additives for food industry applications as well as being able to simultaneously synthesize high yields of lipase.

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Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment

Cited by 15 publications

References 47 publications

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

Simultaneous high nutritional single cell oil and lipase production by Candida viswanathii

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