Isolation and quantification of bioactive Carpaine from <i>Carica papaya</i> L. and its commercial formulation by HPTLC densitometry

Haldar, Sourav; Mohapatra, Satyabrata; Singh, Rahul; Katiyar, Chandra Kant

doi:10.1080/10826076.2020.1725558

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…To further analyze the role of the ten most cited plant species for ciguatera and diarrhea, we have described their ethnobotanical uses in French Polynesia and other Polynesian islands, along their bioactive compounds potentially responsible for the antidiarrheal and ciguatera protective effect (Table 6). Haldar et al, 2020;Panoff, 1966;Pétard, 2019;Singh et al, 1984;Whistler, 1985 (anti-inflammatory, antimicrobial) ascorbic acid (anti-oxidant) Batiha et al, 2020;Girardi et al, 2015;Panoff, 1966;Pétard, 2019;Weimer et al, 2021 Cocos quercetin (antiinflammatory), rutin and scopoletin (antidopaminergic) Abou Assi et al, 2017;Dixon, 1999;Girardi et al, 2015;Horowitz, 2001;Panoff, 1966;Pétard, 2019;Singh et al, 1984;Torres et al, 2017;Whistler, 1985 Musa quercetin and quercitrin (antidiarrheal) Girardi et al, 2015;Naseer et al, 2018;Palombo, 2006;Panoff, 1966;Pétard, 2019;Whistler, 1985 Punica granatum C gallic acid, punicalagin and quercetin (antiinflammatory), rutin (antidopaminergic) quercetin (antiinflammatory), rutin (antidopaminergic) Panoff, 1966;Pétard, 2019;Sameh et al, 2018;Singh et al, 1984;Whistler, 1985 Zingiber officinale C, D Rhizome: stomach pain with chest tightness, ulcer gingerols and shogaols (antiinflammatory, antinausea), zingerone (antidiarrheal) Ali et al, 2008;…”

Section: Benefit-risk Balance Of the Most Cited Treatmentsmentioning

confidence: 99%

Polynesian medicine used to treat diarrhea and ciguatera: An ethnobotanical survey in six islands from French Polynesia

Chassagne

Butaud

Torrente

et al. 2022

Journal of Ethnopharmacology

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Section: Benefit-risk Balance Of the Most Cited Treatmentsmentioning

confidence: 99%

Polynesian medicine used to treat diarrhea and ciguatera: An ethnobotanical survey in six islands from French Polynesia

Chassagne

Butaud

Torrente

et al. 2022

Journal of Ethnopharmacology

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“…The yield of crude alkaloid extract was approximately 0.414 % of the dried leaves weight. Carpaine was reported to be a principal alkaloid of Carica papaya L. which was found to be present 0.52 % in shade-dried leaves [12]. Apart from carpaine, pseudocarpaine, dehydrocarpaine I and II have been found in papaya leaves [2,3], however, the yield of these alkaloids has not been published.…”

Section: Discussionmentioning

confidence: 99%

Spasmolytic Effect of Papaya (Carica papaya L.) Leave Alkaloid on Isolated Rat Myometrial Contraction in vitro

Sawangjaroen

Keereevong

2022

Trends Sci

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This study aimed to investigate the action of crude alkaloid extract from papaya leaves (PA) on the isolated rat myometrium in vitro. The alkaloids were extracted from papaya leaves using conventional acid-base method. The myometrium strips were taken from female Wistar rats pretreated with diethylstilbestrol (100 mg) for 24 h before the experiment. A preliminary experiment has shown that PA had no effect on myometrial contraction. However, PA (10-6 - 10-3 gm/mL) significantly inhibited myometrium contraction pre-contracted with a depolarizing solution in a concentration-dependent fashion. PA (10-5, 3×10-5 or 10-4 gm/mL) or verapamil (10-9, 10-8 and 10-7 M) were able to inhibit the myometrial contractile response to CaCl2 (10-5 - 3×10-2 M) in Ca2+-free solution and cause a rightward shift of log concentration-response curve. PA also significantly inhibited myometrium contraction produced by oxytocin (1 mU/mL), ACh (3×10-5 M) and PGF2a (10-5 M). Furthermore, propranolol (10-7 M) caused no effect on uterine relaxation induced by PA (10-6 - 10-3 gm/mL), although it inhibited uterine relaxation induced by isoproterenol (10-10 - 10-5 M). These results indicated that PA was a uterine relaxant. Its mechanism of action may be unrelated to stimulation of b2-adrenergic receptors of the uterus. This action may be contributed to mainly the Ca2+ influx, possibly through voltage-operated Ca2+ channel on the plasma membrane, and to a lesser extent by a rise in Ca2+ release from internal storage. However, the action of PA at intracellular sites, downstream of Ca2+ influx such as the contractile machinery of the myometrium, cannot be excluded. HIGHLIGHTS Alkaloid extract from Carica papayaleaves has shown to be a uterine relaxant on isolated rat uterus in vitro The alkaloid extract was able to inhibit uterine contraction stimulated by various uterine stimulants such as acetylcholine, oxytocinand prostaglandin F2alpha The inhibition on uterine contraction may be due to the inhibition on calcium influx into the uterine muscle cells GRAPHICAL ABSTRACT

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“…In cosmetics, black hair dyes and face masks can be made from papaya seeds [25] and leaves [26], respectively. In relation to pharmaceutical products, various studies have been conducted to formulate papaya products operating via various routes of administration, including oral [27], topical [28], and transdermal [29]. More specifically, most papaya-based products administered via the oral route were prepared in capsule and tablet dosage forms [30,31], but others, using the liposome delivery system [32] and selfnanoemulsion [33], were also formulated.…”

Section: Introductionmentioning

confidence: 99%

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

et al. 2021

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Carica papaya (papaya) leaf extract has been used for a long time in a traditional medicine to treat fever in some infectious diseases such as dengue, malaria, and chikungunya. The development of science and technology has subsequently made it possible to provide evidence that this plant is not only beneficial as an informal medication, but also that it has scientifically proven pharmacological and toxicological activities, which have led to its formal usage in professional health care systems. The development of formulations for use in nutraceuticals and cosmeceuticals has caused this product to be more valuable nowadays. The use of good manufacturing practice (GMP) standards, along with the ease of registering this product facilitated by policies of the national government, will absolutely increase the value of papaya leaf extract as a vital nutraceutical and cosmeceutical products in the near future. In this article, we review the potential of papaya leaf extract to be a high-value commodity in terms of its health effects as well as its industrial benefits.

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Isolation and quantification of bioactive Carpaine from Carica papaya L. and its commercial formulation by HPTLC densitometry

Cited by 7 publications

References 8 publications

Polynesian medicine used to treat diarrhea and ciguatera: An ethnobotanical survey in six islands from French Polynesia

Polynesian medicine used to treat diarrhea and ciguatera: An ethnobotanical survey in six islands from French Polynesia

Spasmolytic Effect of Papaya (Carica papaya L.) Leave Alkaloid on Isolated Rat Myometrial Contraction in vitro

The Future of Carica papaya Leaf Extract as an Herbal Medicine Product

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