Isolation and purification of plant secondary metabolites using column-chromatographic technique

Bajpai, Vivek K.; Majumder, Rajib; Park, Jae Gyu

doi:10.3329/bjp.v11i4.28185

Cited by 44 publications

(30 citation statements)

References 4 publications

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“…The crude extract obtained from Soxhlet extraction was subjected silica gel (60-120 mesh) column chromatography (10×2 cm column) [14,15] . The adsorbed extract was eluted with hexane and ethyl acetate in the ratio of 3:2 at a flow rate of 3 ml/min.…”

Section: Column Chromatography and Uv Analysismentioning

confidence: 99%

Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

Pratap¹,

Rather²,

Shantaram³

2020

pharmaceutical-sciences

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Section: Column Chromatography and Uv Analysismentioning

confidence: 99%

Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

Pratap¹,

Rather²,

Shantaram³

2020

pharmaceutical-sciences

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“…After chromatographic separation, a total of 128 fractions were obtained and each fraction was spotted on a pre-coated silica gel 60 F254, 0.25 mm thick TLC plate (Merck) and eluted in hexane:ethyl acetate (3:1), the Rf values were calculated for these fractions and the similar ones were pooled together. Rechromatographing of these fractions in a stepwise gradient of ethyl acetate:methanol (9:1) solvent system was performed with a result of getting 89 subfractions [18].…”

Section: Column Chromatographymentioning

confidence: 99%

In Vitro and in Silico Approaches on the Antibacterial Activity of Tinospora Cordifolia Methanolic Stem Extract

Thamizhpriya¹,

A²,

T³

et al. 2020

Asian J Pharm Clin Res

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Objective: The objective of the study was to evaluate the antibacterial activity of methanolic stem fraction of Tinospora cordifolia against Escherichia coli and Staphylococcus aureus by in vitro and in silico approaches. Methods: In agar disc diffusion method, the inhibitory zone produced by various concentrations of the fraction showed a dose-dependent inhibition pattern. Minimum inhibitory concentration (MIC) values were calculated by broth dilution method. The total DNA present in the fraction treated bacterial cultures was estimated and compared with control DNA. The two-dimensional and three-dimensional structures of the gas chromatography– mass spectrometry (GC–MS) identified compounds were generated using ChemSketch tool. The docking studies were performed for analyzing the receptor and ligand interactions. Results: The higher zone revealed the maximum inhibition of the growth of bacteria that were ranged from 2 mm to 6 mm for E. coli and 1.5 mm to 6.3±0.29 mm for S. aureus. MIC values showed that 30 μg/ml of the fraction was found as the effective dose. The DNA content isolated from the treated culture of both the strains was comparatively lesser than that of the untreated control culture. The GC–MS data analysis depicted the presence 15 major components in the fraction and the sharp peaks were obtained at time intervals 17.50, 20.27, 30.06, etc. Conclusion: Thus, methanolic stem fraction of T. cordifolia possesses promising therapeutic activity against the urinary tract infection pathogens such as E. coli and S. aureus and a further exploration in the isolation and characterization such as plant-derived phytoconstituents would open up new ventures in the field of antibacterial drug discovery.

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“…The value of similar retardation factor (R f ), the contents of the test tubes were mixed together for further elution through TLC. TLC is the most applied method of isolation of bioactive compounds from plants [23]. After drying of the sample solution in the test tubes, samples were run over TLC plate using different solvent system composed of different faction of n-hexane, chloroform and ethyl acetate (in terms of polarity increment) and observed under UV lamp in long and short wavelength and spotted properly.…”

Section: Isolation Of Stigmasterolmentioning

confidence: 99%

Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

et al. 2019

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Background: Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia. Methods: The dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then each extract fractions were investigated for their pharmacologic properties. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1 H-NMR and matched with published phytochemistry report. Results: Methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Our present study indicates that methanol extract was most potent (IC 50 = 9.926 μg/ml) inhibitor of DPPH free radicals. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC 50 = 34.493 μg/ml) compared to other plant extracts. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl 3 ) and analyzed by 1 H-NMR. The structure of stigmasterol was confirmed by comparing the 1 H-NMR data with previously published phytochemistry report.Conclusion: Cheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required.

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Isolation and purification of plant secondary metabolites using column-chromatographic technique

Cited by 44 publications

References 4 publications

Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

In Vitro and in Silico Approaches on the Antibacterial Activity of Tinospora Cordifolia Methanolic Stem Extract

Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

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