Isolation and Purification of Kava Lactones by High Performance Centrifugal Partition Chromatography

Mikell, Julie Rakel; Schaneberg, Brian T; Khan, Ikhlas A.

doi:10.1081/jlc-120025423

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…Small polyketides have been reported as MAOIs; for example, desmethoxyyangonin 150 isolated from the medicinal plant Renealmia Alpinia was found to be a MAO inhibitor, being selective toward human MAO-B with an IC50 value of 0.12 μM compared to an IC50 value of 1.85 μM for MAO-A [18]. Desmethoxyyangonin 150 belongs to the well-known kavalactone-type secondary metabolites which are the major constituents isolated predominantly from Piper methysticum roots, the principal ingredient of the kava drink [193]. The kava drink has been used for centuries by the natives of the pacific islands of Vanuatu, Fiji, Tonga, Samoa, and Micronesia due to its psychoactive and medicinal properties.…”

Section: Mao Inhibitors From Miscellaneous Classes Of Natural Productsmentioning

confidence: 99%

Natural Products Inhibitors of Monoamine Oxidases—Potential New Drug Leads for Neuroprotection, Neurological Disorders, and Neuroblastoma

et al. 2022

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Monoamine oxidase inhibitors (MAOIs) are an important class of drugs prescribed for treatment of depression and other neurological disorders. Evidence has suggested that patients with atypical depression preferentially respond to natural product MAOIs. This review presents a comprehensive survey of the natural products, predominantly from plant sources, as potential new MAOI drug leads. The psychoactive properties of several traditionally used plants and herbal formulations were attributed to their MAOI constituents. MAO inhibitory constituents may also be responsible for neuroprotective effects of natural products. Different classes of MAOIs were identified from the natural product sources with non-selective as well as selective inhibition of MAO-A and -B. Selective reversible natural product MAOIs may be safer alternatives to the conventional MAOI drugs. Characterization of MAO inhibitory constituents of natural products traditionally used as psychoactive preparations or for treatment of neurological disorders may help in understanding the mechanism of action, optimization of these preparations for desired bioactive properties, and improvement of the therapeutic potential. Potential therapeutic application of natural product MAOIs for treatment of neuroblastoma is also discussed.

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Section: Mao Inhibitors From Miscellaneous Classes Of Natural Productsmentioning

confidence: 99%

Natural Products Inhibitors of Monoamine Oxidases—Potential New Drug Leads for Neuroprotection, Neurological Disorders, and Neuroblastoma

et al. 2022

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“…In the past, repeated column chromatography with silica as stationary phase seemed to be the only useful method to achieve relatively large amounts of pure compounds. [8,12] More recently, Mikell et al, 2003 [13] described the isolation of the kavalactones applying high performance centrifugal partition chromatography (HPCPC). The collected fractions were analyzed by TLC on silica.…”

Section: Introductionmentioning

confidence: 99%

Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones

Schäfer

Winterhalter

2005

Journal of Liquid Chromatography & Related Technologies

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High speed countercurrent chromatography (HSCCC) was used to isolate the major kavalactones kavain, 7,8-dihydrokavain, methysticin, 7,8-dihydromethysticin, yangonin, and demethoxyyangonin. An ethanolic crude extract of kava root was subjected to HSCCC separation. Due to the presence of polymeric material, the sample load was limited. Hence, gel chromatography was applied in order to concentrate the kavalactones and to reduce the amount of polymers. The purified extract was again subjected to a HSCCC separation. A higher sample input was achieved, but only kavain and demethoxyyangonin were obtained in pure form. The remaining kavalactones eluted in three fractions, which needed to be further purified by preparative HPLC.

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“…Present purification methods include the use of complicated, time‐consuming and labor intensive columns with a variety of stationary phases (Sephadex LH20, silica gel) and sequential solvent extraction 1, 3, 11, 14, 18, 19. As one kind of countercurrent chromatography, high‐performance centrifugal partition chromatography (HPCPC) has been widely applied in many academic researches 20–23. Without the involvement of a solid phase, compounds are separated in HPCPC by partitioning between two immiscible liquid phases.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCPC has several advantages including no irreversible adsorption and complete recovery in samples. With these advantages, HPCPC is gaining popularity as a purification tool for natural products 20–21. In this study, we report a rapid and simple method to produce α‐ and γ‐mangostins in substantial yield and purity, which can be used by researchers for future in vitro and in vivo biological studies.…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of major xanthones from mangosteen pericarp using high‐performance centrifugal partition chromatography

Shan¹,

Zhang²

2010

J of Separation Science

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Mangosteen fruit pericarp (MFP) is a rich source of xanthones, which has shown remarkable pharmacological activities. To isolate xanthones, previous methods included labor intensive and time-consuming solid-phase extractions (Sephadex LH20, silica gel) and sequential solvent extraction. In this study, major xanthones (alpha- and gamma-mangostins) in MFP were isolated at high purity in one step utilizing high-performance centrifugal partition chromatography with solvent system composed of petroleum ether, ethyl acetate, methanol and water (10:5:5:1). In one run, 200 mg crude extract of MFP was injected and 55.4 mg alpha-mangostin and 12.4 mg gamma-mangostin were obtained with the purity of 93.6 and 98.4%, respectively. The yields of them were 86.3 and 76.3%, respectively. As alpha- and gamma-mangostins are reported to show potent antioxidant, anti-inflammatory and anticancer activities, this method can be used for the large-scale production of them for future in vitro and in vivo biological studies.

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Isolation and Purification of Kava Lactones by High Performance Centrifugal Partition Chromatography

Cited by 8 publications

References 5 publications

Natural Products Inhibitors of Monoamine Oxidases—Potential New Drug Leads for Neuroprotection, Neurological Disorders, and Neuroblastoma

Natural Products Inhibitors of Monoamine Oxidases—Potential New Drug Leads for Neuroprotection, Neurological Disorders, and Neuroblastoma

Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones

Preparative separation of major xanthones from mangosteen pericarp using high‐performance centrifugal partition chromatography

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