Isolation and purification of alkaloids from lotus leaves by ionic‐liquid‐modified high‐speed countercurrent chromatography

Wu, Nan; Xie, Huihui; Fang, Yingtong; Liu, Yuanyuan; Xi, Xingjun; Qiao, Chun‐Feng; Dong, Genlai; Lan, Tao; Wei, Yun

doi:10.1002/jssc.201700851

Cited by 22 publications

(6 citation statements)

References 36 publications

(55 reference statements)

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“…HSCCC has the advantages of larger sample loading capacity, complete sample recovery, versatile elution modes because of its liquid stationary phase . So it has been widely used in the separation of target compounds from natural products . However, to our knowledge, there is no report on the separation of MA and OA with HSCCC.…”

Section: Introductionmentioning

confidence: 99%

Continuous separation of maslinic and oleanolic acids from olive pulp by high‐speed countercurrent chromatography with elution‐extrusion mode

Huang

Zhang

Pei

et al. 2019

J of Separation Science

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In this work, a continuous high-speed countercurrent chromatography method has been developed on the basis of elution-extrusion mode and this method was successfully applied to the separation of maslinic and oleanolic acid from the extract of olive pulp. In the process of 'elution', the sample solution was continuously loaded into the column and the maslinic acid was steadily eluted out in this step while highly retained oleanlic acid always stayed in the column. In the process of 'extrusion', the oleanlic acid was pushed out of the column with the stationary phase. In this way, we achieved a large sample loading. A total of 120 mL sample solution (about 89.55% of the column volume) which contains 600 mg olive pulp extract was pumped in the apparatus by a constant-flow pump and the maslinic and oleanolic acids were largely separated within 120 min. Both of these two compounds presented high yields and high purities (271.6 mg for maslinic acid with 86.7% and 83.9 mg oleanolic acids with 83.4%).

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Section: Introductionmentioning

confidence: 99%

Continuous separation of maslinic and oleanolic acids from olive pulp by high‐speed countercurrent chromatography with elution‐extrusion mode

Huang

Zhang

Pei

et al. 2019

J of Separation Science

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“…Herbal products and their extracts are often used in the food and cosmetics industries. Conventional extraction methods are generally considered as time and solvent consuming, 11 and often expensive equipment is required, e.g., ionic liquid‐based microwave‐assisted extraction, 12 pH zone‐refining countercurrent chromatography, 13 and ionic liquid‐modified high‐speed countercurrent chromatography 14 . Hence, more convenient and eco‐friendly methods are required.…”

Section: Introductionmentioning

confidence: 99%

An eco‐friendly extraction and purification method of nuciferine from Folium nelumbinis with p‐sulfonatocalix[6]arenes

Chen

et al. 2022

Phytochemical Analysis

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Introduction Folium nelumbinis is used as vegetable, functional food and herbal medicine in Asia. p‐Sulfonatocalix[6]arene (SC6A) is a water‐soluble supramolecular macrocycle and has never been applied to the extraction of herbal products. Objective In this study, SC6A‐assisted extraction of nuciferine from Folium nelumbinis has been carried out to develop an eco‐friendly extraction process with high extraction efficacy and easy operation. Methods Single‐factor experiments were adopted to obtain the optimal conditions for the SC6A‐assisted extraction of nuciferine from Folium nelumbinis, and then nuciferine and SC6A were separated easily by one‐step alkalization. The host–guest complexes between nuciferine and SC6A were analyzed by competitive fluorescence titration, DSC, FT‐IR and 1H‐NMR. Results The optimal SC6A/Folium nelumbinis/solution ratio for extraction was 0.4:1:20 (g/g/mL), with a granulometric fraction below 180 μm and an extraction time of 1 h with soaking. The purity and recovery of nuciferine extracted with SC6A were increased 29.24 and 35.73 times compared with extraction with aqueous solution, respectively. Moreover, a good reusability of SC6A in the extraction of nuciferine was demonstrated. Competitive fluorescence titration, DSC, FT‐IR and 1H‐NMR characterization indicated that SC6A could form host–guest complexes with nuciferine at a ratio of 1:1. Conclusion The study provided an eco‐friendly, safe and effective nuciferine extraction method, which can be used for the development of nutrition supplements containing nuciferine.

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“…High‐speed counter‐current chromatography (HSCCC) is a continuous liquid–liquid partition chromatography without solid support. This novel chromatographic technique has many advantages such as the elimination of irreversible adsorption, high sample recovery, low risk of sample denaturation, and large preparative capacity . HSCCC has recently become a useful tool for the preparative isolation and purification of various natural products .…”

Section: Introductionmentioning

confidence: 99%

Application of coordination agent in high‐speed counter‐current chromatography for the preparative separation and isolation ginkgolic acids from the sarcotesta of Ginkgo biloba L

Zhu

Jiang

et al. 2018

J of Separation Science

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An efficient coordination high‐speed counter‐current chromatography method for the preparative separation of ginkgolic acids from the sarcotesta of Ginkgo biloba L was developed. The type, concentration, and mechanism of the coordination agent were investigated. Following the use of four types of metal salts including silver nitrate, copper chloride, ferric chloride, and aluminium nitrate, n‐heptane/ethyl acetate/methanol/acetic acid 5:4:1:1, v/v with 0.20 mol/L silver nitrate as the coordination agent was chosen as the optimum two‐phase solvent system. Five main ginkgolic acids including C13:0, C15:0, C15:1, C17:1, and C17:2 were successfully separated with purities greater than 98%. The sample loading was 500 mg, the flow‐rate was 2.0 mL/min, rotation speed was 800 rpm and temperature was 20°C. The structures of the separated ginkgolic acids were identified by comparison with standard samples and electrospray ionization mass spectrometry. The introduction of coordination chemistry in high‐speed counter‐current chromatography is novel and effective for the preparative separation and isolation of ginkgolic acids from the sarcotesta of Ginkgo biloba L and could also be applied to separate compounds which form coordination bonds in other complex natural products.

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Isolation and purification of alkaloids from lotus leaves by ionic‐liquid‐modified high‐speed countercurrent chromatography

Cited by 22 publications

References 36 publications

Continuous separation of maslinic and oleanolic acids from olive pulp by high‐speed countercurrent chromatography with elution‐extrusion mode

Continuous separation of maslinic and oleanolic acids from olive pulp by high‐speed countercurrent chromatography with elution‐extrusion mode

An eco‐friendly extraction and purification method of nuciferine from Folium nelumbinis with p‐sulfonatocalix[6]arenes

Application of coordination agent in high‐speed counter‐current chromatography for the preparative separation and isolation ginkgolic acids from the sarcotesta of Ginkgo biloba L

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