Isolation and Purification of a Small-Molecular-Weight GTP-Binding Protein from Plants

Sommer, Debbie; Song, Pill‐Soon

doi:10.1006/prep.1994.1058

Cited by 5 publications

(3 citation statements)

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“…In particular, both heterotrimeric and small G proteins contribute to light signal transduction (20)(21)(22)(23)(24). In oat, the Pr form of phytochrome enhances the GTPase activity of a small Ras-like G protein (24).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

Shen¹,

Han²,

Kim³

et al. 2008

BMB Reports

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Nucleoside diphosphate kinase (NDPK) is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Arabidopsis NDPK2, like its mammalian counterparts, is multifunctional despite its initial discovery phytochrome-interacting protein. This similarity raises the possibility that NDPK2 may play a role in G-protein signaling in plants. In the present study, we explore the potential relationship between NDPK2 and the small G proteins, Pra2 and Pra3, as well as the heterotrimeric G protein, GPA1. We report a physical interaction between NDPK2 and these small G proteins, and demonstrate that NDPK2 can stimulate their GTPase activities. Our results suggest that NDPK2 acts as a GTPase-activating protein for small G proteins in plants. We propose that NDPK2 might be a missing link between the phytochromemediated light signaling and G protein-mediated signaling.[BMB reports 2008; 41(9): 645-650]

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Section: Introductionmentioning

confidence: 99%

Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

Shen¹,

Han²,

Kim³

et al. 2008

BMB Reports

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“…Binding of [35S]GTP'/S was performed using a method described previously (14). Briefly, following electrophoresis on 12% SDS-polyacrylamide gel, proteins were transferred to a nitrocellulose sheet and incubated for 1.5 h at room temperature in 100ml of 50raM Tris-HCl (pH 7.5) containing 0.1% BSA, 5mM MgC12, 2mM DTT, and 0.1% (v/v) Triton X-100.…”

Section: Binding Of [35s]gtpys After Electrophoretic Transfer To Nitrmentioning

confidence: 99%

The potential role for Cdc42 protein from rat brain cytosol in phospholipase D activation

et al. 1998

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Phospholipase D (PLD) has been extracted from rat brain membranes and chromatographically enriched 70-fold. From the rat brain cytosol, Cdc42 with a Mr of about 24,000 and ADP-ribosylation Factor (Art) with a Mr of about 18,000 have been purified to near homogeneity. PLD was activated better by purified cytosolic Arf than by the other small G proteins tested. Cdc42 purified from rat brain cytosol showed 70% of PLD activation activity exerted by cytosolic Arf, suggesting that Cdc42 may be one of the major G proteins involved in the activation of membrane-associated PLD. While Cdc42 or RhoA exhibited synergistic activation of PLD when administered in conjunction with Arf, Cdc42 and RhoA showed an additive effect when used together. It is possible that Art" and Rho family proteins may have different interaction sites on PLD. These findings support a role for GTP-binding proteins of the Rho family as well as Arf in the activation of membrane-associated PLD and further suggest that Cdc42 may be a major G protein involved in the PLD activation in rat brain.

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“…Homologues of the heterotrimeric G-protein subunits have been cloned from plants (60-63), although none has been shown to be directly involved in light signaling. However, homologues of small GTP-binding proteins, similar to the animal ras-related superfamily involved in various modes of signal transduction, have been cloned and the expression of some of these are modulated by light (64)(65)(66)(67)(68).…”

Section: G-proteins Cgmp and Calcium/calmodulinmentioning

confidence: 99%

Light Signal Transduction in Plants

Staub

Deng

1996

Photochem & Photobiology

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Isolation and Purification of a Small-Molecular-Weight GTP-Binding Protein from Plants

Cited by 5 publications

References 0 publications

Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

The potential role for Cdc42 protein from rat brain cytosol in phospholipase D activation

Light Signal Transduction in Plants

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