Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

Shen, Yu; Han, Yun‐Jeong; Kim, Jeong-Il; Song, Pill‐Soon

doi:10.5483/bmbrep.2008.41.9.645

Cited by 15 publications

(10 citation statements)

References 45 publications

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“…Arabidopsis NDPK2, like its mammalian counterparts, is multifunctional despite its initial discovery phytochrome-interacting protein. Resent reports suggest that NDPK2 acts as a GTPase-activating protein for small G proteins in plants and they propose that NDPK2 might be a missing link between the phytochrome mediated light signaling and G protein-mediated signaling [36]. In this study, NDPK was upregulated by SCMV infection, suggesting that NDPK was also involved in plant-virus interaction.…”

Section: Resultssupporting

confidence: 52%

Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus

Wang

Chen

et al. 2013

PLoS ONE

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BackgroundSugarcane mosaic virus (SCMV) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. Control strategies have been developed, but only marginal successes have been achieved. For the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required.Methodology/Principal FindingsThe responses of resistant and susceptible genotypes of maize to SCMV and the molecular basis of the resistance were studied using a proteomic approach based on two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS/MS) analysis. Ninety-six protein spots showed statistically significant differences in intensity after SCMV inoculation. The classification of differentially expressed proteins showed that SCMV-responsive proteins were mainly involved in energy and metabolism, stress and defense responses, and photosynthesis. Most of the proteins identified were located in chloroplasts, chloroplast membranes, and the cytoplasm. Analysis of changes in phytohormone levels after virus inoculation suggested that salicylic acid, abscisic acid, jasmonic acid, and azelaic acid may played important roles in the maize response to SCMV infection.Conclusions/SignificanceAmong these identified proteins, 19 have not been identified previously as virus-responsive proteins, and seven were new and did not have assigned functions. These proteins may be candidate proteins for future investigation, and they may present new biological functions and play important roles in plant-virus interactions. The behavioural patterns of the identified proteins suggest the existence of defense mechanisms operating during the early stages of infection that differed in two genotypes. In addition, there are overlapping and specific phytohormone responses to SCMV infection between resistant and susceptible maize genotypes. This study may provide important insights into the molecular events during plant responses to virus infection.

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Section: Resultssupporting

confidence: 52%

Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus

Wang

Chen

et al. 2013

PLoS ONE

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“…By this reaction, NDPK controls the level of GTP, which is, for example, required for the conversion of inactive GDP-bound GTPases to the active GTP-bound form. NDPK2, another isoform of NDPK in Arabidopsis, was shown to bind and stimulate the GTPase activity of small G proteins and serve as a signal transducer in phytochrome signaling (Choi et al, 1999;Shen et al, 2008). Interestingly, it was previously shown that NDPK activity is required for dynamin-dependent endocytosis in Drosophila (Krishnan et al, 2001) and that the level of GTP-bound dynamin proteins controls the level of endocytosis (Sever et al, 2000).…”

Section: Two Kinases With Putative Regulatory Functionsmentioning

confidence: 99%

In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes

et al. 2009

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Peroxisomes are metabolically diverse organelles with essential roles in plant development. The major protein constituents of plant peroxisomes are well characterized, whereas only a few low-abundance and regulatory proteins have been reported to date. We performed an in-depth proteome analysis of Arabidopsis (Arabidopsis thaliana) leaf peroxisomes using onedimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry. We detected 65 established plant peroxisomal proteins, 30 proteins whose association with Arabidopsis peroxisomes had been previously demonstrated only by proteomic data, and 55 putative novel proteins of peroxisomes. We subsequently tested the subcellular targeting of yellow fluorescent protein fusions for selected proteins and confirmed the peroxisomal localization for 12 proteins containing predicted peroxisome targeting signals type 1 or 2 (PTS1/2), three proteins carrying PTS-related peptides, and four proteins that lack conventional targeting signals. We thereby established the tripeptides SLM. and SKV. (where . indicates the stop codon) as new PTS1s and the nonapeptide RVx 5 HF as a putative new PTS2. The 19 peroxisomal proteins conclusively identified from this study potentially carry out novel metabolic and regulatory functions of peroxisomes. Thus, this study represents an important step toward defining the complete plant peroxisomal proteome.Surrounded by single membranes, peroxisomes are small, ubiquitous eukaryotic organelles mediating a wide range of oxidative metabolic activities that vary by the species, cell type, and environmental conditions in which the organism lives (Beevers, 1979;Van den Bosch et al., 1992). Plant peroxisomes are essential to physiological processes such as lipid metabolism, photorespiration, and plant hormone biosynthesis and metabolism (Olsen and Harada,

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“…Similar to NME proteins in mammals, NDPK isoforms in plants also perform diverse important roles [49]. NDPK2 from Arabidopsis thaliana was proposed to function as a GAP for small GTPases Pra2 (RabA3) and Pra3 (RabA4), which belong to the Rab family [50]. Active Rab5 and Rab7 play crucial roles in promoting the interaction of vesicles with lysosomes [51,52].…”

Section: Gap Activity Of Nme Proteinsmentioning

confidence: 99%

Modulation of small GTPase activity by NME proteins

Filić

Marinović

Šoštar

et al. 2018

Laboratory Investigation

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NME proteins are reported to influence signal transduction activity of small GTPases from the Ras superfamily by diverse mechanisms in addition to their generic NDP kinase activity, which replenishes the cytoplasmic pool of GTP. Comprehensive evidence shows that NME proteins modulate the activity of Ras GTPases, in particular members of the Rho family, via binding to their major activators GEFs. Direct interaction between several NMEs and Ras GTPases were also indicated in vitro and in vivo. These modes of regulation are mainly independent of the NME's kinase activity. NMEs also modulate the Ras-mediated signal transduction by interfering with the formation of a Ras signaling complex at the plasma membrane. In several examples, NMEs were proposed to perform the role of GAP proteins by promoting hydrolysis of the bound GTP, but this activity still requires additional verification. Early suggestions that NMEs can activate small GTPases by direct phosphorylation of the bound GDP, or by high-rate loading of GTP onto a closely apposed GTPase, were largely dismissed. In this review article, we survey and put into perspective published examples of identified and hypothetical mechanisms of Ras signaling modulation by NME proteins. We also point out involvement of NMEs in the transcriptional regulation of components of Ras GTPases-mediated signal transduction pathways, and reciprocal regulation of NME function by small GTPases, particularly related to NME's binding to membranes.

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Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

Cited by 15 publications

References 45 publications

Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus

Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus

In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes

Modulation of small GTPase activity by NME proteins

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