Isolation and Properties of a Protein Complex Containing Flagellar Movement-Initiating Phosphoprotein from Testes of a White Salmon

Jin, Zong Xuan; Nakajima, Tetsuo; Morisawa, Masaaki; Hayashi, Hiroshi

doi:10.1093/oxfordjournals.jbchem.a124374

Cited by 15 publications

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“…18 kDa and 15 kDa proteins are similar in molecular mass to the 15 kDa protein that plays an important role in initiation process of sperm motility in salmonids (Morisawa and Hayashi, 1986;Hayashi et al, 1987;Jin et al, 1994), although phosphorylation in the latter protein occurred at tyrosine residues. A 15 kDa protein was phosphorylated during initiation of motility of chum salmon sperm, in contrast to the dephosphorylation of 15 kDa and 18 kDa proteins seen in tilapia sperm under hypo-osmotic conditions.…”

Section: Discussionmentioning

confidence: 97%

“…Both adenylate cyclase activity and the cAMP concentration increase on motility initiation in intact sperm (Morisawa and Ishida, 1987), and cAMP is necessary for reactivating the demembranated sperm (Morisawa and Okuno, 1982). It has been suggested that a cAMP dependent phosphorylation cascade is the main cause of motility activation (Hayashi et al, 1987;Jin et al, 1994). In tilapia, however, cAMP failed to activate the motility of demembranated sperm, which was activated by Ca 2+ .…”

Section: Discussionmentioning

confidence: 99%

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Requirement of Ca2+ on activation of sperm motility in euryhaline tilapiaOreochromis mossambicus

Morita

Takemura

Okuno

2003

Journal of Experimental Biology

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It is widely accepted that sperm motility in teleosts is regulated by, and suited to, the environment in which they reproduce. In many teleosts, which spawn in either freshwater or seawater, sperm motility is initiated by osmotic shock when sperm are ejaculated. These osmolality dependent regulatory systems are quite different in freshwater cyprinid teleosts, such as carp, gold fish and zebrafish, and marine teleosts, such as the pufferfish and flounder. Sperm of freshwater teleosts are quiescent at the osmolality of seminal plasma, referred to as isotonic conditions (approximately 300 mosmol kg -1 ). They begin to move when suspended in hypotonic water (<300 mosmol kg -1 ) and show high motility (as % motile sperm) in freshwater. By contrast, sperm of marine teleosts begin to move when released into hypertonic water (>300 mosmol kg -1 ), and show the highest motility at approximately 1000 mosmol kg -1 , which is almost equivalent to seawater (Morisawa and Suzuki, 1980;Oda and Morisawa, 1993). It has therefore been assumed that the difference in osmolality between seminal plasma and the external water, corresponding to the spawning ground, is an important regulatory factor of sperm motility in many teleosts. If this is the case, the regulatory system of sperm motility seems to be reversed in freshwater and seawater teleosts, since the osmolality of seminal plasma is intermediate between that of freshwater and seawater.The tilapia Oreochromis mossambicus, a euryhaline teleost, has the can acclimate to wide range of salinities, from freshwater to seawater, by a mechanism that includes chloride cells (Sakamoto et al., 1997;Balm et al., 1994;Borski et al., 1994). A unique feature of tilapia is that they can reproduce in both freshwater and seawater, even though there is a large osmotic difference between those two environments (Brock, 1954). Thus, it has been assumed that tilapia sperm can swim independently of osmolality, or modulate their regulatory mechanism to suit fertilization in either high or low salinity. It is possible that the motility regulatory system of tilapia sperm is quite different from those reported for other teleosts. We have already reported that tilapia sperm acclimated to seawater were motile in hypertonic water, i.e. seawater, in the presence of Ca 2+ , whereas in tilapia acclimated to freshwater sperm could not swim in hypertonic conditions even in the presence of Ca 2+ (Morita and Okuno, 1998). Linhart et al. (1999)

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Requirement of Ca2+ on activation of sperm motility in euryhaline tilapiaOreochromis mossambicus

Morita

Takemura

Okuno

2003

Journal of Experimental Biology

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“…With regard to sperm motility, phosphorylation of a tyrosine residue with a 15-kDa axonemal protein, called a flagellar movement-initiating phosphoprotein (MIPP) (Jin et al, 1994), by tyrosine kinase has been found to play a role in the initiation of flagellar movement of quiescent spermatozoa of the rainbow trout (Hayashi et al, 1987;Morisawa and Hayashi, 1985). In addition, it is suggested that in this system the tyrosine kinase might be activated by a cAMP-dependent protein kinase (Hayashi et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Effects of tyrosine kinase inhibitor on the motility and ATP concentrations of fowl spermatozoa

1998

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The possible role of tyrosine kinase in the regulation of fowl sperm motility was investigated by using a stable analogue of erbstatin, methyl 2,5‐dihydroxycinnamate (2,5‐MeC), a specific inhibitor of tyrosine kinase. This inhibited the motility of intact spermatozoa at 30°C in a dose‐dependent manner. In contrast, the motility of demembranated spermatozoa was not inhibited by the same concentrations of 2,5‐MeC. At 40°C, both intact and demembranated spermatozoa were almost immotile with or without 2,5‐MeC. Additionally, intact spermatozoa, stimulated by the addition of Ca2+ or calyculin A, a specific inhibitor of protein phosphatases, lost their motility with the subsequent addition of 2,5‐MeC at 40°C. However, unlike the motility, the ATP concentrations of spermatozoa were maintained in about 30–35 nmol ATP/109 cells during these incubation periods. The activity of tyrosine kinase of spermatozoa at 30°C, estimated by measuring the phosphorylation of a synthetic peptide substrate, RR‐SRC, was 0.17 pmol/min per milligram of protein. This activity was lower than that of fowl testes or chick brain but higher than that of chick liver. These results suggest that tyrosine kinase activity, which is not retained in the axoneme and/or accessory cytoskeletal components, may be involved in the maintenance of flagellar movement of fowl spermatozoa at 30°C. Mol. Reprod. Dev. 49:196–202, 1998. © 1998 Wiley‐Liss, Inc.

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“…Again, the link between cAMP increase and motility initiation at the axoneme level was mainly investigated in Salmonidae (Jin et al . ). The cAMP and Ca 2+ , as second messengers, play key roles in the initiation of sperm motility in fish (Zilli et al .…”

Section: Inhibition and Activation Of Sperm Motility In Salmonidaementioning

confidence: 97%

“…In freshwater spawning fish, spermatozoa experience a hypo-osmotic change, that is a transfer into a medium with lower osmolality, as they are released from the seminal plasma into freshwater, a medium where their motility may last less than two minutes after activation (Alavi & Cosson 2005;Gasparini et al 2010). Again, the link between cAMP increase and motility initiation at the axoneme level was mainly investigated in Salmonidae (Jin et al 1994). The cAMP and Ca 2+ , as second messengers, play key roles in the initiation of sperm motility in fish (Zilli et al 2008).…”

Section: Inhibition and Activation Of Sperm Motility In Salmonidaementioning

confidence: 99%

Protein phosphorylation and ions effects on salmonid sperm motility activation

Figueroa

Cosson²,

Lee‐Estévez

et al. 2017

Reviews in Aquaculture

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Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and activation of sperm motility are prerequisite processes for the contact and fusion of male and female gametes at fertilization. Many proteins are involved in the activation of sperm motility in many species. Cell signalling for the initiation of sperm motility in the salmonid fish has drawn much attention during the last two decades. In some species, protein phosphorylation process was shown to be involved in flagellar motility regulation. Hyperpolarization of the sperm membrane induces synthesis of cAMP (cyclic AMP), which triggers further cell signalling processes, such as cAMP‐dependent protein phosphorylation that finally initiates sperm motility in salmonid fish. Ions such as Na+, K+ and Ca2+ play also an important role in the activation of sperm motility in many species, more specifically in salmonids. Salmonid fish sperm motility can be suppressed by millimolar concentrations of extracellular K+, and dilution of K+ upon spawning is enough to trigger the cAMP‐dependent signalling cascade leading to motility initiation. This review aims to update the present knowledge about the roles of ions and protein phosphorylation process in the sperm motility activation in salmonids.

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Isolation and Properties of a Protein Complex Containing Flagellar Movement-Initiating Phosphoprotein from Testes of a White Salmon

Cited by 15 publications

References 0 publications

Requirement of Ca2+ on activation of sperm motility in euryhaline tilapiaOreochromis mossambicus

Requirement of Ca2+ on activation of sperm motility in euryhaline tilapiaOreochromis mossambicus

Effects of tyrosine kinase inhibitor on the motility and ATP concentrations of fowl spermatozoa

Protein phosphorylation and ions effects on salmonid sperm motility activation

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