Isolation and polymerase chain reaction detection of virulence invA gene in Salmonella spp. from poultry farms in Jos, Nigeria

Anejo-Okopi, Joseph; Isa, Samson Ejiji; Audu, Onyemocho; Fagbamila, Idowu Oluwabunmi; Iornenge, Jacob Chire; Smith, Ifeanyi Stella

doi:10.4103/2276-7096.192237

Cited by 16 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We reported a 21.4% prevalence of Salmonella in commercial poultry in Ibadan. Previous studies have reported the prevalence of Salmonella infection in poultry farms and chickens in Nigeria to be between 2.0% and 43.0%, 17 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 and this is similar to the prevalence reported in another study conducted in Bangladesh. 33 Salmonella have been implicated in bacteraemia, septicaemia and gastroenteriti s in humans, and their presence in poultry chickens may pose serious health risks to humans.…”

Section: Discussionsupporting

confidence: 85%

Occurrence and antimicrobial susceptibility patterns of Salmonella species from poultry farms in Ibadan, Nigeria

2022

View full text Add to dashboard Cite

Background: Salmonella species are among the major foodborne pathogens causing diseases of economic and public health implications in poultry and humans globally.Objective: This study aimed to determine the occurrence and antimicrobial susceptibility patterns of Salmonella isolates from chickens in poultry farms in Ibadan, Southwest Nigeria.Methods: Cloacal swab samples (n = 360) were obtained from chickens randomly selected from 10 poultry farms in five local government areas of Ibadan, Oyo State, from 04 April 2018 to 20 November 2018. Bacterial identification and antimicrobial susceptibility testing were performed using established protocols. Data were analysed using descriptive statistics and Pearson’s chi-squared test at P ≤ 0.05 significance level.Results: The overall prevalence of Salmonella was 21.4%. There were statistically significant associations between Salmonella prevalence and the farm location (p = 0.003), age of chickens (p 0.001), and health status of chickens (p 0.001). All Salmonella isolates (n = 77; 100.0%) were resistant to cefuroxime. The isolates were also highly resistant to cotrimoxazole (n = 74; 96.1%), chloramphenicol (n = 73; 94.8%), meropenem (n = 72; 93.5%), gentamicin (n = 69; 89.6%), and tetracycline (n = 64; 83.1%).Conclusion: The presence of drug-resistant Salmonella in commercial layer chickens in Ibadan is a potential threat to consumer health as it increases the risk of carcass contamination and pathogen propagation, and limits the options to control and treat infections in humans and animals. Well-integrated national surveillance systems for monitoring Salmonella and antimicrobial resistance in poultry are critical.

show abstract

Section: Discussionsupporting

confidence: 85%

Occurrence and antimicrobial susceptibility patterns of Salmonella species from poultry farms in Ibadan, Nigeria

2022

View full text Add to dashboard Cite

show abstract

“…Salmonella isolates detected by PCR in this study produced bands with amplicon size of 284bp. This corroborates with the work done by Nwiyi et al (2016) and that done by Anejo-Okopi et al(2016).The outcome of the PCR result from the 3 isolates was 3/3 (100%) compared to the phenotypic method by culture 3/360 (0.8%) used (Plate 1). This result suggests that the detection of invAgene by PCR is faster, more sensitive and specific than the conventional methods, and it a good confirmatory method for the detection of Salmonella spp.…”

Section: Type Of Sample Sourcesupporting

confidence: 91%

Occurrence and characterization of Salmonella isolates in raw eggs from quail and chicken in selected poultry farms in Jos, Plateau State, Nigeria

Bose¹,

Joshua²,

Audu³

et al. 2020

J. Vet. Med. Anim. Health

View full text Add to dashboard Cite

The study was carried out in three Local Government Areas: Jos North, Jos South and Jos East. For each egg type, twelve (12) samples each were collected from five (5) farms. A total of 360 samples were randomly collected consisting of equal number of quail and chicken eggs (180 each). A well-structured questionnaire was used to help analyze the results. Samples were examined for the presence of Salmonella isolates using standard microbiological practices. Isolates were confirmed using biochemical tests, and molecular characterization (using specific primers). Isolates were also tested for antimicrobial susceptibility by disc diffusion method. Results showed that 3(1.7%) chicken eggs were positive for Salmonella infection whereas no positive result was recorded from quail eggs. This resulted in a total prevalence of 0.9%. Bukuru and Zawan (Jos South) were the only farm locations with Salmonella positive cases with 1(8.3%) and 2(16.7%) respectively. Although the present finding has found low prevalence of salmonellosis in chicken and quail egg in the study area, there is need for constant monitoring on regular basis to avert health risks associated with consuming Salmonellae infected poultry products in endemic areas. The three (3) isolates were Salmonella Gallinarum and gave agglutination reaction with polyvalent O antisera and no reaction with polyvalent H antisera. Polymerase Chain Reaction (PCR) results confirmed all the three (3) isolates that were successfully amplified using specific primers, thus supporting phenotypic outcome. The information provided in this report is crucial to all stakeholders including the poultry farmers, consumers and regulators of chicken products.

show abstract

“…(Darwin and Miller, 1999). Many studies detected invA gene in 100% of salmonella serovars as recorded in Egypt by (Karmi, 2013 andAmmar et al, 2016) and in international countries as in reports of (Samanta et al, 2014and Chaudhary et al, 2015 and with (91.0%) in Nigeria by (Anejo-Okopi et al, 2016).Many protein effectors of the type III secretion system (TTSSs) such as avrA and sopE usually involved in most of gram negative bacteria as salmonella species (Cornelis and Van Gijsegem, 2000)which responsible for impairment of IL-8 and TNF-α inflammatory cells and macrophages and subsequently increase bacterial virulence properties (Ben-Barak et al 2006). Our results revealed that avrA gene was detected in all nine examined serotypes (100%).…”

Section: Discussionmentioning

confidence: 90%

Molecular Detection of Some Virulence Genes of Salmonella Serotypes Isolated From Poultry in Egypt

ElSheikh¹,

Abdeen

Ammar

2019

Journal of Current Veterinary Research

View full text Add to dashboard Cite

Salmonellosis is recognized as an important health problem and a major challenge in poultry industry worldwide. This study highlights the prevalence rate of salmonella serotypes in chickens as well as demonstration of some critical virulence genes by using specific primers sets in PCR reaction. Out of 100 freshly dead chickens, sixteen chicken samples were positive to Salmonella with 16% prevalence rate.The rate of recovery of Salmonellae from the different internal organs showed that the intestine is the most predication seat followed by, heart and liver with (8%), (6%) and (2%), respectively. Salmonella isolates (16) from chickens were serotyping using O and H antigen. The results revealed that S. Enteritidis was the most serotype found in 5 isolates (31.25%) followed by S. Infantis in 4 isolates (25%). Both S. Newlands and S. Kentucky found in 2 isolates 12.5%. Each of S. Ferruch, S. Weybridge and S. Naestved have one isolate (6.25%) .The application of PCR for detection of invA, avrA and stn virulence genes in nine salmonella serovars revealed that these genes (invA, avrA and stn)were detected in 100% of tested strains except S. Naestved does not contain stn gene. Hence, this study recommends that PCR may be used for rapid and sensitive detection of Salmonella and help in understanding the role of these genes in pathogenicity of salmonella infection in poultry.

show abstract

Isolation and polymerase chain reaction detection of virulence invA gene in Salmonella spp. from poultry farms in Jos, Nigeria

Cited by 16 publications

References 0 publications

Occurrence and antimicrobial susceptibility patterns of Salmonella species from poultry farms in Ibadan, Nigeria

Occurrence and antimicrobial susceptibility patterns of Salmonella species from poultry farms in Ibadan, Nigeria

Occurrence and characterization of Salmonella isolates in raw eggs from quail and chicken in selected poultry farms in Jos, Plateau State, Nigeria

Molecular Detection of Some Virulence Genes of Salmonella Serotypes Isolated From Poultry in Egypt

Contact Info

Product

Resources

About