Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica

Yang, Chu-Fang; Lee, Chi-Mei; Wang, Chun-Chin

doi:10.1016/j.chemosphere.2005.05.012

Cited by 54 publications

(42 citation statements)

References 13 publications

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“…Among chlorinated phenols pentachlorophenol (PCP) and its sodium salt have been widely used as wood and leather preservative owing to their toxic effect on bacteria, mould, algae and fungi (Kaoa et al 2004). PCP is toxic to all forms of life since it is an inhibitor of oxidative phosphorylation (Shen et al 2005;Yang et al 2006). The US Environmental protection agency (EPA) has listed PCP as a priority contaminant because of its proven carcinogenicity and toxicity (Bock et al 1996).…”

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confidence: 99%

“…The aromatic ring is thus totally dechlorinated prior to ring cleavage (Wang et al 1998;Tartakovsky et al 1999Tartakovsky et al , 2001. Aerobic degradation of PCP has also been studied extensively and several bacterial strains capable of degradation have been reported such as Flavobacterium, Arthrobacter, Pseudomonas, and Sphingomonas (Edgehill and Finn 1983;Crawford and Mohn 1985;Saber and Crawford 1985;Xun and Orser 1991;Orser et al 1993;Edgehill 1994;Miethling and Karlson 1996;Chanama and Crawford 1997;Leung et al 1999;Thakur et al 2002;Yang et al 2006;Dams et al 2007).…”

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Characterization of Pentachlorophenol Degrading Bacterial Consortium from Chemostat

Sharma

Thakur

2008

Bull Environ Contam Toxicol

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A microbial consortium was developed by continuous enrichment of bacterial population isolated from sediment core of pulp and paper mill effluent in mineral salts medium (MSM) supplemented with pentachlorophenol (PCP) as sole source of carbon and energy in the chemostat. The enriched consortium contained three bacterial strains identified as Escherichia coli (PCP1), Pseudomonas aeruginosa (PCP2) and Acinetobacter sp. (PCP3) by morphological and biochemical tests, further confirmation was done using 16S rDNA sequence analysis. The potency of bacterial isolates in degradation of PCP was monitored in terms of growth and utilization of PCP as substrate with spectrophotometer and gas chromatograph-mass spectrometer (GC-MS) analysis. The strains were tested for their utilization of various organic compounds. The strain PCP3, showed higher potency to utilize PCP as sole source of carbon and energy than PCP1 and PCP2. The bacterial strain were able to utilize PCP through an oxidative and reductive route as indicated with the formation of tetrachloro-p-hydroquinone (TeCH), 2-chloro-1,4-benzenediol and 2,3,4,6-tetrachlorophenol, respectively.

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Characterization of Pentachlorophenol Degrading Bacterial Consortium from Chemostat

Sharma

Thakur

2008

Bull Environ Contam Toxicol

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“…Pentachlorophenol (PCP) is used as a biocide in leather tanning process, and is capable of being biodegradable by only a limited number of bacteria [11,12]. Apart from Cr(VI), PCP is highly toxic, and a recalcitrant organic compound because of its stable aromatic ring system and high chloride content, thereby persisting in the environment [13].…”

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Isolation and Growth Characteristics of Chromium(VI) and Pentachlorophenol Tolerant Bacterial Isolate from Treated Tannery Effluent for its Possible Use in Simultaneous Bioremediation

et al. 2011

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The bacterial strains resistant to pentachlorophenol (PCP) and hexavalent chromium [Cr(VI)] were isolated from treated tannery effluent of a common effluent treatment plant. Most of the physico-chemical parameters analyzed were above permissible limits. Thirty-eight and four bacterial isolates, respectively were found resistant to [50 lg/ml concentration of [Cr(VI)] and the same level of PCP. Out of the above 42 isolates, only one was found simultaneously tolerant to higher levels of both PCP (500 lg/ml) and Cr(VI) (200 lg/ml), and hence was selected for further studies. To the best of our knowledge, this is the first report in which a native bacterial isolate simultaneously tolerant to such a high concentrations of Cr(VI) and PCP has been reported. The culture growth was best at 0.4% (w/v) glucose as an additional carbon source and 0.2% (w/v) ammonium chloride as a nitrogen source. The growth results with cow urine as a nitrogen source were comparable with the best nitrogen source ammonium chloride. The isolate exhibited resistance to multiple heavy metals (Pb, As, Hg, Zn, Co & Ni) and to antibiotics nalidixic acid and polymixin-B. The efficacy of bacterial isolate for growth, PCP degradation (56.5%) and Cr(VI) bioremediation (74.5%) was best at 48 h incubation. The isolate was identified as Bacillus sp. by morphological and biochemical tests. The 16S rDNA sequence analysis revealed 98% homology with Bacillus cereus. However, further molecular analysis is underway to ascertain its likelyhood of a novel species.

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“…Many species/strains of sphingomonads degrading aromatic and/or xenobiotic compounds have been isolated (27), and the potential usefulness of sphingomonads for on-site degradation has also been demonstrated (40). In spite of increasing interest in the behavior and distribution of sphingomonads in the field, not much information on their ecology has been collected.…”

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Abundance and Community Structure of Sphingomonads in Leaf Residues and Nearby Bulk Soil

2010

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We examined the abundance and community structure of sphingomonads in the decaying leaf residues of eight plant species as well as the nearby soil, by 16S rRNA gene-based real-time PCR and denaturing gradient gel electrophoresis. In the leaf residues, the sphingomonads generally accumulated to high levels, comprising approximately 15% of the total bacteria, and formed a community structure related to sampling locations. At least within the time period studied, their abundance in leaf residues changed, but their community structure was basically maintained. In soil, sphingomonads made up only 1.7% of total bacteria on average. The community structure of sphingomonads differed between the leaf residues and bulk soil, among plant plots, and among samples collected at different times. The results show that particular sphingomonad populations accumulate in leaf residues compared to the surrounding bulk soil under field conditions.

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Isolation and physiological characterization of the pentachlorophenol degrading bacterium Sphingomonas chlorophenolica

Cited by 54 publications

References 13 publications

Characterization of Pentachlorophenol Degrading Bacterial Consortium from Chemostat

Characterization of Pentachlorophenol Degrading Bacterial Consortium from Chemostat

Isolation and Growth Characteristics of Chromium(VI) and Pentachlorophenol Tolerant Bacterial Isolate from Treated Tannery Effluent for its Possible Use in Simultaneous Bioremediation

Abundance and Community Structure of Sphingomonads in Leaf Residues and Nearby Bulk Soil

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