Isolation and Physiological Activities of Piericidin A, A Natural Insecticide Produced by<i>Streptomyces</i>

Tamura, Saburo; Takahashi, Nobutaka; Miyamoto, Sadaaki; Mori, Rinpei; Suzuki, Saburô; Nagatsu, Junsaku

doi:10.1080/00021369.1963.10858144

Cited by 41 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Piericidins exhibit interesting biological activities, in particular antitrypanosomal [82]. In our research, compounds 3-7 exhibited different degrees of cytotoxicity on three types of tumor cells, but they did not show any antibacterial activity, which was consistent with previous reports [78,81]. As a Piericidins-producing strain, NEAU-wh3-1 has certain application potential in pest control.…”

Section: Discussionsupporting

confidence: 90%

“…Bioassay-guided isolation of the active components of the strain yielded eight main bioactive compounds. Compounds 2-8 are known compounds, which structures were elucidated as conglobatin (2) [75], piericidin C1 (3) [76], piericidin C5 (4) [77], piericidin A1 (5) [78], piericidin A3 (6) [76], Mer-A 2026 A (7) [79], and BE-52211 D (8) [80] by analysis of their spectroscopic data and comparison with literature values (Figure 5, Figures S12-S27). Compound 1 is a new zincophorin analogue ( Figure 6, Figures S4-S11) [17].…”

Section: Structural Elucidationmentioning

confidence: 99%

See 1 more Smart Citation

Taxonomic Characterization and Secondary Metabolite Analysis of NEAU-wh3-1: An Embleya Strain with Antitumor and Antibacterial Activity

et al. 2020

View full text Add to dashboard Cite

Cancer is a serious threat to human health. With the increasing resistance to known drugs, it is still urgent to find new drugs or pro-drugs with anti-tumor effects. Natural products produced by microorganisms have played an important role in the history of drug discovery, particularly in the anticancer and anti-infective areas. The plant rhizosphere ecosystem is a rich resource for the discovery of actinomycetes with potential applications in pharmaceutical science, especially Streptomyces. We screened Streptomyces-like strains from the rhizosphere soil of wheat (Triticum aestivum L.) in Hebei province, China, and thirty-nine strains were obtained. Among them, the extracts of 14 isolates inhibited the growth of colon tumor cell line HCT-116. Strain NEAU-wh-3-1 exhibited better inhibitory activity, and its active ingredients were further studied. Then, 16S rRNA gene sequence similarity studies showed that strain NEAU-wh3-1 with high sequence similarities to Embleya scabrispora DSM 41855T (99.65%), Embleya hyalina MB891-A1T (99.45%), and Streptomyces lasii 5H-CA11T (98.62%). Moreover, multilocus sequence analysis based on the five other house-keeping genes (atpD, gyrB, rpoB, recA, and trpB) and polyphasic taxonomic approach comprising chemotaxonomic, phylogenetic, morphological, and physiological characterization indicated that the isolate should be assigned to the genus Embleya and was different from its closely related strains, therefore, it is proposed that strain NEAU-wh3-1 may be classified as representatives of a novel species of the genus Embleya. Furthermore, active substances in the fermentation broth of strain NEAU-wh-3-1 were isolated by bioassay-guided analysis and identified by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses. Consequently, one new Zincophorin analogue together with seven known compounds was detected. The new compound showed highest antitumor activity against three human cell lines with the 50% inhibition (IC50) values of 8.8–11.6 μg/mL and good antibacterial activity against four pathogenic bacteria, the other known compounds also exhibit certain biological activity.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Structural Elucidationmentioning

confidence: 99%

Taxonomic Characterization and Secondary Metabolite Analysis of NEAU-wh3-1: An Embleya Strain with Antitumor and Antibacterial Activity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Piericidin A1, α-pyridone antibiotics, was first isolated from the type strain of Streptomyces mobaraensis [20]. This compound is mainly isolated from various Streptomyces species from both terrestrial and marine origins [21].…”

Section: Discussionmentioning

confidence: 99%

Marine Streptomyces chumphonensis KK1-2T produces piericidin A1 as the major secondary metabolite

Phongsopitanun¹,

Kanchanasin²,

Khanboon³

et al. 2021

ScienceAsia

View full text Add to dashboard Cite

Strain KK1-2 T is the type strain of marine Streptomyces chomphonensis. Since the discovery of this Streptomyces species, the secondary metabolite of the type strain has not been characterized. Based on genome sequence analysis, the biosynthetic gene cluster of piericidin A1 was detected in the genome of strain KK1-2 T . The isolation of secondary metabolites and spectroscopy data confirm the chemical structure of piericidin A1 produced by the type strain. This study is the first report of the genome analysis and the secondary metabolite produced by the type strain of S. chumphonensis.

show abstract

“…Piericidin A was purified via bioactivity-guided fractionation approaches and its structure and chemical properties were characterized. Notably, this same study found that piericidin A presented limited cytotoxicity against Gram-negative bacteria such as E. coli and Xanthymonas oryzae [50]. In 1966, piericidin A was investigated for antibiotic properties against Gram-positive bacteria [51].…”

Section: Piericidin Amentioning

confidence: 97%

“…Piericidin A was originally discovered for its insecticidal properties in 1963 ( Figure 5) [50]. Soil samples were collected from Chiba Prefecture, a region encompassing the outskirts of Tokyo, and their microbial makeup was analyzed for their toxicity against a variety of larval species.…”

Section: Piericidin Amentioning

confidence: 99%

Natural Product Type III Secretion System Inhibitors

Pendergrass

May

2019

Antibiotics

View full text Add to dashboard Cite

Many known inhibitors of the bacterial type III secretion system (T3SS), a virulence factor used by pathogenic bacteria to infect host cells, are natural products. These compounds, produced by bacteria, fungi, and plants, may have developed as prophylactic treatments for potential attack by bacterial pathogens or as an attempt by symbiotic organisms to protect their hosts. Regardless, better understanding of the structures and mechanisms of action of these compounds may open opportunities for drug development against diseases caused by pathogens utilizing the T3SS. This review will cover selected known natural products of the T3SS and detail what is known of their origin and mechanism of action. These inhibitors highlight nature’s ability to modulate interactions between organisms at a cellular level.

show abstract

Isolation and Physiological Activities of Piericidin A, A Natural Insecticide Produced byStreptomyces

Cited by 41 publications

References 0 publications

Taxonomic Characterization and Secondary Metabolite Analysis of NEAU-wh3-1: An Embleya Strain with Antitumor and Antibacterial Activity

Taxonomic Characterization and Secondary Metabolite Analysis of NEAU-wh3-1: An Embleya Strain with Antitumor and Antibacterial Activity

Marine Streptomyces chumphonensis KK1-2T produces piericidin A1 as the major secondary metabolite

Natural Product Type III Secretion System Inhibitors

Contact Info

Product

Resources

About