Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals

Pauciullo, Alfredo; Kubı́čková, Svatava; Černohorská, Halina; Petrova, Kristina; Berardino, D. Di; Ramunno, L.; Rubeš, Jiřı́

doi:10.17221/5541-vetmed

Cited by 16 publications

(13 citation statements)

References 31 publications

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“…This methodology was effective in isolating centromeric repetitive sequences in previous studies (Li et al, 2005;Pauciullo et al, 2006), including rodents (Louzada et al, 2008).…”

Section: A Novel Satellite Dna Family In Peromyscus Eremicusmentioning

confidence: 99%

A novel satellite DNA sequence in the Peromyscus genome (PMSat): Evolution via copy number fluctuation

Louzada

Vieira-da-Silva

Mendes-da-Silva

et al. 2015

Molecular Phylogenetics and Evolution

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“…This methodology was effective in isolating centromeric repetitive sequences in previous studies (Li et al, 2005;Pauciullo et al, 2006), including rodents (Louzada et al, 2008).…”

Section: A Novel Satellite Dna Family In Peromyscus Eremicusmentioning

confidence: 99%

A novel satellite DNA sequence in the Peromyscus genome (PMSat): Evolution via copy number fluctuation

Louzada

Vieira-da-Silva

Mendes-da-Silva

et al. 2015

Molecular Phylogenetics and Evolution

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“…Species-specific clones were selected by DOT BLOT hybridization following Pauciullo et al [2006], fluorescently labeled and checked by FISH. Subsequently, plasmid DNA was isolated and sequenced.…”

Section: Chromosome Painting and Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Molecular Insights into X;BTA5 Chromosome Rearrangements in the Tribe Antilopini (Bovidae)

Černohorská

Kubı́čková

Váhala³

et al. 2012

Cytogenet Genome Res

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For a clade that includes Antilope, Gazella,Nanger and Eudorcas (Antilopinae), X;BTA5 translocation is a synapomorphy. Using a combination of fluorescence in situ hybridization (FISH) probes and polymerase chain reaction techniques, we provide (i) the first insight into the X;BTA5 architecture which differs in the species under study: Antilope cervicapra (genus Antilope), Gazella leptoceros (genus Gazella) and Nanger dama ruficollis (genus Nanger), (ii) determination of interstitial satellite DNA at the X;BTA5 junctions, and (iii) determination of repetitive sequences occupying constitutive heterochromatin of Xp arms in the studied species. The distribution of 2 repetitive DNA families in the centromeric regions of all chromosomes has been investigated by FISH with probes representing satellite I and satellite II DNA in all studied species. In this context, we discuss a markedly smaller centromere in the BTA5 (Y2) unfused chromosomes in males in the XY1Y2 determining system in comparison with other acrocentrics. An analysis of karyotypic data described in current published studies revealed a disparity with the data determined by FISH. In this report, we document chromosomal fusions in the 3 species mentioned resulting from FISH with painting probes prepared from cattle (Bos taurus). The number and chromosomal location of nucleolus organizer regions were determined by FISH. In the present study, we emphasize the importance of chromosomal rearrangement verification, particularly, if they are used for phylogenetic analysis.

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“…Specific clones were selected by dot blot hybridization following Pauciullo et al [2006], fluorescently labeled and hybridized back to dik-dik chromosomes. This revealed 2 different hybridization patterns: one was specific to the centromeric regions (i.e.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Cytotypes of Kirk’s Dik-Dik (<i>Madoqua kirkii</i>,Bovidae) Show Multiple Tandem Fusions

Černohorská

Kubı́čková

Váhala³

et al. 2010

Cytogenet Genome Res

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Madoqua kirkii, a miniature African antelope, is noted for extensive chromosomal variation that has been categorized in four distinct cytotypes (A–D). In this investigation, we analyzed the A cytotype (2n = 46, FN = 48) using a suite of molecular cytogenetic approaches that entailed (i) whole chromosome and subchromosomal painting by fluorescence in situ hybridization (FISH), (ii) the study of Madoqua centromeric-specific DNA derived from pooled DNA obtained from the centromeric regions of the acrocentric chromosomes, and (iii) DNA from the telomere:centromere junctions of tandemly fused chromosomes. DNA from these sources was used to probe for the persistence of interstitial satellite DNA and residual centromeric sequences in the tandem and centric fusion junctions by PCR and FISH. The analyses show centromeric sequences at two of the six tandem fusion junctions. These data, and those of hybrid specimens (A × B cytotypes) in conjunction with published information permitted an interpretation of the probable sequence of chromosomal rearrangements among the M. kirkii cytotypes. We discuss the findings in the context of chromosomal evolution in these antelopes, and the implications that these hold for ex-situ breeding programs of the species.

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Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals

Cited by 16 publications

References 31 publications

A novel satellite DNA sequence in the Peromyscus genome (PMSat): Evolution via copy number fluctuation

A novel satellite DNA sequence in the Peromyscus genome (PMSat): Evolution via copy number fluctuation

Molecular Insights into X;BTA5 Chromosome Rearrangements in the Tribe Antilopini (Bovidae)

Cytotypes of Kirk’s Dik-Dik (<i>Madoqua kirkii</i>,Bovidae) Show Multiple Tandem Fusions

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