Molecular Insights into X;BTA5 Chromosome Rearrangements in the Tribe Antilopini (Bovidae)

Černohorská, Halina; Kubı́čková, Svatava; Váhala, J.; Rubeš, Jiřı́

doi:10.1159/000336248

Cited by 22 publications

(21 citation statements)

References 64 publications

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“…Blood samples were cultured, harvested and fixed according to standard protocols as described previously [ 35 ]. Metaphase chromosome spreads for laser microdissection as well as slides for FISH analysis were prepared according to the procedures described previously [ 36 ]. For the assessment of deer karyotypes, GTG-banding was performed using the standard trypsin/Giemsa procedure [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…The analysis was performed following the protocols for FISH with painting or BAC probes. The posthybridization washing, image capture and processing were carried out as described in [ 36 ]. The BAC probes labelled with biotin-16-dUTP and digoxigenin-11-dUTP were detected with Avidin-CY3 (Amersham Pharmacia Biotech, Piscataway, NJ, USA) and antidigoxigenin-fluorescein (Roche).…”

Section: Methodsmentioning

confidence: 99%

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Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

et al. 2017

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The Cervidae family comprises more than fifty species divided into three subfamilies: Capreolinae, Cervinae and Hydropotinae. A characteristic attribute for the species included in this family is the great karyotype diversity, with the chromosomal numbers ranging from 2n = 6 observed in female Muntiacus muntjak vaginalis to 2n = 70 found in Mazama gouazoubira as a result of numerous Robertsonian and tandem fusions. This work reports chromosomal homologies between cattle (Bos taurus, 2n = 60) and nine cervid species using a combination of whole chromosome and region-specific paints and BAC clones derived from cattle. We show that despite the great diversity of karyotypes in the studied species, the number of conserved chromosomal segments detected by 29 cattle whole chromosome painting probes was 35 for all Cervidae samples. The detailed analysis of the X chromosomes revealed two different morphological types within Cervidae. The first one, present in the Capreolinae is a sub/metacentric X with the structure more similar to the bovine X. The second type found in Cervini and Muntiacini is an acrocentric X which shows rearrangements in the proximal part that have not yet been identified within Ruminantia. Moreover, we characterised four repetitive sequences organized in heterochromatic blocks on sex chromosomes of the reindeer (Rangifer tarandus). We show that these repeats gave no hybridization signals to the chromosomes of the closely related moose (Alces alces) and are therefore specific to the reindeer.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

et al. 2017

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“…Therefore, we cannot affirm that the amount of ITS observed is sufficient to isolate the autosomal and sexual segments, but it may contribute to the insulation buffer. These observations lead us to search for other repetitive sequences that could be colocated with ITSs and block the diffusion of X inactivation to the autosomal segment, such as rDNA [Dobigny et al, 2004;RodriguesNoronha et al, 2004;Cernohorska et al, 2012Cernohorska et al, , 2015. In this case, rDNA clusters have already been ruled out since they do not map to the centromeric region of Rb(X.1) in M. minutoides [Cazaux et al, 2011].…”

Section: Role Of Its As a Buffer Between Autosomal And Sex Chromosomementioning

confidence: 99%

Extensive Amplification of Telomeric Repeats in the Karyotypically Highly Diverse African Pygmy Mice

Colomina¹,

Catalan²,

Britton‐Davidian³

et al. 2017

Cytogenet Genome Res

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Telomeres are ribonucleoprotein structures protecting the physical ends of eukaryotic chromosomes. However, telomeric sequences can also occur at non-terminal regions of chromosomes, forming the so-called interstitial telomeric sequences (ITSs). Some ITSs are considered as relics of past chromosomal rearrangements and as such provide important insights into karyotype evolution. By FISH, we explored the distribution of telomeric motifs in the genome of a complex of mammalian species that has long been recognized for its extraordinary karyotypic diversity: the African pygmy mice. This survey involved 5 species, representing 10 highly diverse karyotypes with or without autosomal and sex-autosome robertsonian (Rb) fusions. The study revealed that in species with an ancestral-like karyotype (i.e., no fusions; Mus mattheyi and M. indutus), only terminal telomeres were observed, whereas in species experiencing intense chromosomal evolution (e.g., M. minutoides, M. musculoides), a large amplification of telomeric repeats was also identified in the pericentromeric region of acrocentrics and most metacentrics. We concluded that (i) the mechanism of Rb fusion in the African pygmy mice is different than the one highlighted in the house mouse; (ii) the intensity of the ITS hybridization signal could be a signature of the age of formation of the Rb fusion; (iii) the large amplification of pericentromeric telomeric sequences in acrocentrics may mediate the formation of Rb fusions, and (iv) the ITSs on the sex-autosome fusion Rb(X.1) may participate to the insulation buffer between the sexual and autosomal arms to prevent X inactivation from spreading and silencing autosomal genes and allow the independent regulation of replication timing of both segments.

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“…Interestingly, all species of Tragelaphini exhibit Y;BTA13 translocation, which would result in an imbalance in diploid numbers between sexes with the exception of T. spekii (2n = 30) and T. imberbis (2n = 38) [Rubes et al, 2008]. For a clade that includes Antilope, Gazella, Nanger and Eudorcas (Antilopinae), X;BTA5 translocation is a synapomorphy [Cernohorska et al, 2012]. Using region-specific painting probes, the authors revealed that tandem fusion (head-totail) assembled the 2 chromosomes in G. leptoceros and N. dama ruficollis , unlike centric fusion (head-to-head) detected in A. cervicapra .…”

Section: Family Bovidaementioning

confidence: 99%

Comparative Molecular Cytogenetics in Cetartiodactyla

Rubeš

Musilová

Kopečná

et al. 2012

Cytogenet Genome Res

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Cetartiodactyla comprises Artiodactyla (even-toed ungulates) and Cetacea (whales, dolphins and porpoises). Artiodactyla is a large taxon represented by about 200 living species ranked in 10 families. Cetacea are classified into 13 families with almost 80 species. Many publications concerning karyotypic relationships in Cetartiodactyla have been published in previous decades. Formerly, the karyotypes of closely related species were compared by chromosome banding. Introduction of molecular cytogenetic methods facilitated comparative mapping between species with highly rearranged karyotypes and distantly related species. Such information is a prerequisite for the understanding of karyotypic phylogeny and the reconstruction of the karyotypes of common ancestors. This study summarizes the data on chromosome evolution in Cetartiodactyla, mainly derived from molecular cytogenetic studies. Traditionally, phylogenetic relationships of most groups have been estimated using morphological data. However, the results of some molecular studies of mammalian phylogeny are discordant with traditional conceptions of phylogeny. Cetartiodactyls provide several examples of incongruence between traditional morphological and molecular data. Such cases of conflict include the relationships of the major clades of artiodactyls, the relationships among the extant families of the suborder Ruminantia or the phylogeny of the family Bovidae. The most unexpected aspect of the molecular phylogeny was the recognition that Cetacea is a deeply nested member of Artiodactyla. The largest living order of terrestrial hoofed mammals is the even-toed hoofed mammals, or Artiodactyla. The artiodactyls are composed of over 190 living species including pigs, peccaries, hippos, camels, llamas, deer, pronghorns, giraffes, sheep, goats, cattle and antelopes. Cetacea is an order of wholly aquatic mammals, which include whales, dolphins and porpoises. Cetartiodactyla has become the generally accepted name for the clade containing both of these orders.

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Molecular Insights into X;BTA5 Chromosome Rearrangements in the Tribe Antilopini (Bovidae)

Cited by 22 publications

References 64 publications

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

Extensive Amplification of Telomeric Repeats in the Karyotypically Highly Diverse African Pygmy Mice

Comparative Molecular Cytogenetics in Cetartiodactyla

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