Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

Sood, Ashwani; Pereira, Dennis; Weissman, Sherman M.

doi:10.1073/pnas.78.1.616

Cited by 346 publications

(109 citation statements)

References 36 publications

(23 reference statements)

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…This fragment spans exon 2 of the c-myc gene. Human MHC class I heavy chain transcripts were detected with an HLA 67 cDNA probe (Sood et al, 1981). Rat MHC class I heavy chain transcripts were detected with a fragment comprising the first 820 bp of the mouse H-2Kd cDNA (Lalanne et al, 1983) using low stringency hybridization (40% formamide at 42OC).…”

Section: Methodsmentioning

confidence: 99%

N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma

Bernards

Dessain

Weinberg

1986

Cell

271

151

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma

Bernards

Dessain

Weinberg

1986

Cell

271

151

View full text Add to dashboard Cite

“…The negative control is DNA digested with HindIII. The positive control MHC class I probe is a 1.4-kb fragment from plasmid pHLA-B7 containing a human HLA-B7 cDNA sequence (33). The specific probe for HLA-A2 is a 490-bp PvuII-MspI subclone of the 3Ј untranslated region (UTR) of the HLA-A2 genomic clone (Ref.…”

Section: Probes For Nuclear Run-on and Northern Analysesmentioning

confidence: 99%

A 3′-Transcribed Region of the HLA-A2 Gene Mediates Posttranscriptional Stimulation by IFN-γ

Snyder

Waring

Sheng

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

The expression of several MHC class I genes is up-regulated at the transcriptional level by IFN-γ. Posttranscriptional mechanisms also have been implicated, but not well characterized. To investigate the mechanism of IFN-γ stimulation of the human MHC class I gene HLA-A2, several human tumor cell lines were transfected with reporter gene constructs driven by the HLA-A2 promoter. We have previously shown that the extended 525-bp HLA-A2 promoter alone, which includes a 5′ IFN-stimulated response element consensus sequence, is not sufficient for IFN-γ response in either K562 or Jurkat cells. In the current study, stable transfection of a genomic HLA-A2 gene construct, containing both 5′- and 3′-flanking sequences, resulted in stimulation of the gene by IFN-γ. Nuclear run-on assays revealed that, unlike other class I genes, IFN-γ stimulation of HLA-A mRNA accumulation occurs almost entirely through posttranscriptional mechanisms. RNA stability assays showed that the effect is not mediated by alteration of the half-life of the HLA-A2 mRNA. Formation of the 3′ end was unaffected by IFN-γ treatment. Sequences that mediate the majority of IFN-γ induction of HLA-A2 mRNA reside in a 127-bp 3′-transcribed region of the gene. This region contains the terminal splice site, the usage of which is not affected by IFN-γ treatment. These results demonstrate a novel posttranscriptional mechanism of regulation of MHC class I genes by IFN-γ.

show abstract

“…bEstimation made from curve giving base pair number of human chromosomes (23) as a function of their relative fluorescence (Fig. 3).…”

Section: (*Imentioning

confidence: 99%

Swine chromosomes: Flow sorting and spot blot hybridization

et al. 1986

View full text Add to dashboard Cite

Flow cytometry analysis was applied to swine chromosomes prepared from phytohemagglutinin (PHA) stimulated peripheral blood lymphocytes. Flow karyotypes from both sexes and from t(3;7) translocation carrier females were obtained. A certain number of chromosome pairs could be assigned to various peaks. In fact, 13 peaks were observed for 18 autosomal pairs plus X and Y. Moreover, abnormalties owing to the t(3;7) translocation were readily observable.The number of base pairs for chromosomes associated with the various peaks was estimated by comparison with human flow karyotypes.The following four peaks were thus sorted: (1) the peak assumed to represent the translocated chromosome 7 plus the normals associated with it; (2) the corresponding peak from a normal swine; (3) the peak assumed to contain among others the normal chromosome 7; and finally (4) the peak corresponding to swine chromosome 1.Chromosomes of each peak were collected on Pall Biodyne membrane. Following appropriate denaturation and prehybridization, the four samples were hybridized with a human leucocyte antigen (HLA) class I32P‐labelled cDNA probe, representing most of the coding sequence of the HLA B7 gene. The results confirmed previous data from other techniques that assigned the swine MHC(SLA) to chromosome 7.Subsequently, sorted samples were hybridized with a porcine genomic Interferon alpha probe in order to confirm the mapping of this gene family on porcine chromosome 1.

show abstract

Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

Cited by 346 publications

References 36 publications

N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma

N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma

A 3′-Transcribed Region of the HLA-A2 Gene Mediates Posttranscriptional Stimulation by IFN-γ

Swine chromosomes: Flow sorting and spot blot hybridization

Contact Info

Product

Resources

About