Isolation and partial characterization of two antifungal proteins from barley

Identification of a 28,000 Dalton endochitinase in barley endosperm

“…The SDS-PAGE also revealed a single distinct band corresponding to 28 kD, verifying that the protein C had been purified to homogeneity. The yield of the purified protein C was 82 lxg/g grain, which agreed with that previously reported by HEJ-GAARD et al (8) and ROBERTS et al (17).…”

“…Recently it has been shown (13) that protein C is synthesized in the starchy endosperm during the late ( -30 days post anthesis) seed development like the protein synthesis inhibitor, PSI, described by ASANO et al (2). ROBERTS et al (17) observed that a barley seed 28 kD protein and PSI inhibit fungal growth. This 28 kD protein (equal to protein C) inhibits growth of Tricoderma reesi very efficiently by addition of as little as 0.1 Bg of protein to a mycelial invasion-inhibition assay.…”

“…Until now, no biological function has been determined for the 60,000 Dalton protein Q. Protein C with a molecular weight of 28 kD has recently been shown to inhibit the growth of various fungi (17). It was concluded that this protein did not function as a translation inhibitor like PSI, which also exhibits antifungal activity.…”

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

“…Chitinases from barley seed belong to class I and II 145,181,283,329 . A barley seed chitinase, 28 kDa and pI 9.7, efficiently inhibited the growth of T. reesei, Phycomyces blakesleeanus, Alternaria alternaria and a protoplastforming mutant of Neurospora crassa 283 , while the 33 kDa chitinase, pI 9.8, inhibited the growth of T. viride, and had lysozyme activity, as measured by the lysis of cell walls of Micrococcus lysodeikticus 145 .…”

“…A barley seed chitinase, 28 kDa and pI 9.7, efficiently inhibited the growth of T. reesei, Phycomyces blakesleeanus, Alternaria alternaria and a protoplastforming mutant of Neurospora crassa 283 , while the 33 kDa chitinase, pI 9.8, inhibited the growth of T. viride, and had lysozyme activity, as measured by the lysis of cell walls of Micrococcus lysodeikticus 145 . A basic 26 kDa barley seed chitinase (CHI26) inhibited the growth of T. reesei and F. sporotrichioides both by itself and also synergistically with glucanase and RIP 181 .…”