Isolation and Molecular Identification of Leishmania spp. Agents in Patients with Cutaneous Leishmaniasis in Yazd Province, Endemic Region of Central Iran

Abstract

Background: Cutaneous Leishmaniasis (CL) is a major health problem in many parts of Iran. Many methods have been introduced for detection and identification of Cutaneous Leishmaniasis. The purpose of this study was isolation and molecular identification of Leishmania spp. agents in patients with CL from endemic region of central Iran. In this study, one of the main loci of central Iran named Yazd will be assessed CL identification using PCR-RFLP. Methods: For this cross-sectional study, sampling was done from 372 suspicious patients with CL who referred to Health Centers of Yazd Province from 2016 to 2017. After collection samples of patients, DNA extraction was done from samples on slides. Genus detection was done using specific primers by PCR. RFLP analysis was done for species identification. Results: Out of 372 samples, 159 samples were positive using PCR based method. Out of 159 samples, 87 (54.7%) L. major and 72 (45.3%) L. tropica were identified using RFLP analysis. The number of lesions in each patient was different but 119 (74.8%) patients showed the number of 1-3 lesions, and more lesions (more than 10 lesions) was showed in 4 (2.5%) person. Conclusion: The CL found in Yazd province resulted from L. major and L. tropica as the agents of rural and urban types, respectively. The prevalence of L. major and L. tropica was almost the same. This indicated that control programs could be designed for treatment and vector and reservoir control.

Comparison of Three Different Therapies for Cutaneous Leishmaniasis and Identification of the Etiologic Isolates in Isfahan, Iran

“…35 Eslami et al in Yazd, Iran, identified 49.01% of samples to be L. major and 50.98% to be L. tropica. 36 People traveling and moving over the time have caused epidemiological changes and created new foci in some parts of Iran. For example, a focus of L. major was created in Pakdasht, Tehran due to people traveling to Sabzevar city (a focus of L. major).…”

Epidemiological Study and Reservoir Identification of Cutaneous Leishmaniasis From Ardestan in Isfahan, Iran (2015-2016)

“…Twice a week, sandflies from inside buildings (bedrooms, covered warehouses, and corridors) and the opened areas (rodent nests, cracks in walls, and bird nests) were caught using 30 glue traps (white papers measuring 15 × 20 cm impregnated with the castor oil). 23 In all cases, the next morning after trapping, adhesive traps were collected before sunrise and transferred to the laboratory for the identification of the mosquitoes species. For this purpose, first, they were identified by entomological experts using organological tests, and then the sandflies were carefully collected from the traps with a dissection needle.…”

Relationship of Leishmania RNA Virus (LRV) and treatment failure in clinical isolates of Leishmania major

“…To detect and identify Leishmania spp., a diagnostic key based on restriction banding pattern of ITS1 was used based on specific primer pair of LITSr-F 5′-CTG GAT CAT TTT CCgATg-3′ and L5.8 s-R 5′-TGA TAC CAC TTA TCG CAC TT-3′ [20]. Amplification reaction mixture included 1x PCR buffer, 0.2 mM dNTP, 1.5 mM MgCl 2 , 1.5 U Taq DNA polymerase, 0.5 µM each primer pair, and 100 ng DNA.…”