Isolation and molecular characterization of<i>Candida africana</i>from Jos, Nigeria

Nnadi, Nnaemeka Emmanuel; Ayanbimpe, Grace Mebi; Scordino, Fabio; Okolo, Mark Ojogba; Enweani, Ifeoma Bessie; Criseo, Giuseppe; Romeo, Orazio

doi:10.3109/13693786.2012.662598

Cited by 35 publications

(23 citation statements)

References 21 publications

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“…In agreement with the existing literature (reviewed in reference 22), C. africana was isolated exclusively in this study from female genital specimens. Indeed, C. africana constituted 6% of C. albicans species complex isolates from female genital specimens and was much more prevalent than the related C. dubliniensis in such samples, as has been reported previously from both Europe and the African subcontinent (18,41). Phenotypically, C. africana differed from C. albicans and C. dubliniensis by the delayed formation of hyphae and absence of chlamydospores in Dalmau cultures, the inability to assimilate trehalose, the lack of N-acetyl-galactosaminidase activity, and the development of smaller, more intensely colored turquoise-green colonies on CHROMagar.…”

Section: Discussionmentioning

confidence: 48%

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

Borman¹,

Székely²,

Linton³

et al. 2013

J Clin Microbiol

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Candida africana was previously proposed as a new species within the Candida albicans species complex, together with C. albicans and C. dubliniensis, although further phylogenetic analyses better support its status as an unusual variant within C. albicans. Here we show that C. africana can be distinguished from C. albicans and C. dubliniensis by pyrosequencing of a short region of ITS2, and we have evaluated its occurrence in clinical samples by pyrosequencing all presumptive isolates of C. albicans submitted to the Mycology Reference Laboratory over a 9-month period. The C. albicans complex constituted 826/1,839 (44.9%) of yeast isolates received over the study period and included 783 isolates of C. albicans, 28 isolates of C. dubliniensis, and 15 isolates of C. africana. In agreement with previous reports, C. africana was isolated exclusively from genital specimens, in women in the 18-to-35-year age group. Indeed, C. africana constituted 15/251 (6%) of "C. albicans" isolates from female genital specimens during the study period. C. africana isolates were germ tube positive, grew significantly more slowly than C. albicans and C. dubliniensis on conventional mycological media, could be distinguished from the other members of the C. albicans complex by appearance on chromogenic agar, and were incapable of forming chlamydospores. Here we present the detailed evaluation of epidemiological, phenotypic, and clinical features and antifungal susceptibility profiles of United Kingdom isolates of C. africana. Furthermore, we demonstrate that C. africana is significantly less pathogenic than C. albicans and C. dubliniensis in the Galleria mellonella insect systemic infection model.

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Section: Discussionmentioning

confidence: 48%

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

Borman¹,

Székely²,

Linton³

et al. 2013

J Clin Microbiol

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“…Also, a significant geographical variation in prevalence of C. africana has been reported from the African continent. Low rates of 2 and 2.4% from Senegal and Nigeria, respectively, have been reported . On the other hand, Tietz et al .…”

Section: Discussionmentioning

confidence: 97%

Multilocus sequence typing of Candida africana from patients with vulvovaginal candidiasis in New Delhi, India

Sharma

Muralidhar

et al. 2014

Mycoses

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We investigated the prevalence of vulvovaginal candidiasis due to C. africana in an STD clinic in India and analysed the genetic relatedness of these C. africana isolates with those outside India. A total of 283 germ-tube-positive yeasts were identified by VITEK2. Molecular characterisation of all isolates was carried out by hwp1-gene-specific PCR. Of 283 germ-tube-positive yeast isolates, four were identified as C. africana using hwp1-gene-specific PCR. All hwp1 PCR positive C. africana were subjected to antifungal susceptibility testing, ITS and D1/D2 region sequencing and were typed by using MLST approach. Similar to C. africana isolates from the United Kingdom and unlike those from Africa, the Indian C. africana grew at 42°C. Sequencing of eight gene fragments in MLST identified all four strains to have different genotypes not reported previously. Furthermore, though the Indian C. africana isolates were susceptible to most of the 14 tested antifungal drugs, differences in susceptibility were observed among the four strains. Our results indicate genetic and phenotypic heterogeneity among C. africana from different geographical regions. Due to lack of data on epidemiology and genetic variability of this under-reported yeast, more studies using molecular methods are warranted.

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“…However, currently C. africana is known as a biovariant of C. albicans with an exceptional capacity to colonize human genitalia and cause mainly vaginal infections (Romeo and Criseo, 2011; Romeo et al, 2013). Its distribution appears to be worldwide, with cases of infection reported from China (Shan et al, 2014; Hu et al, 2015), Japan (Odds et al, 2007), South Korea (Song et al, 2014), Colombia (Rodríguez-Leguizamón et al, 2015), Argentina (Theill et al, 2016), Chile (Odds et al, 2007), India (Sharma et al, 2014), Iran (Yazdanparast et al, 2015), Africa (Tietz et al, 2001; Dieng et al, 2012; Nnadi et al, 2012; Ngouana et al, 2015), USA (Romeo et al, 2013), and Europe (Alonso-Vargas et al, 2008; Romeo and Criseo, 2009; Borman et al, 2013). However, despite the efforts made so far, it is rather difficult to discriminate C. africana from C. albicans in clinical diagnostic laboratories and therefore its epidemiology is still unclear and needs more investigation (Romeo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana

et al. 2017

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This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating-type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this amino sugar in this yeast. Multi-locus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb, SYA1 and VPS13 loci which contributed significantly to the classification of our set of C. africana strains into six existing diploid sequence types. Amplified fragment length polymorphism fingerprint analysis yielded greater genotypic heterogeneity among the C. africana strains. Overall the data reported here show that in C. africana genetic diversity occurs and the existence of this intriguing group of C. albicans strains with specific phenotypes associated could be useful for future comparative studies in order to better understand the genetics and evolution of this important human pathogen.

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Isolation and molecular characterization ofCandida africanafrom Jos, Nigeria

Cited by 35 publications

References 21 publications

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

Multilocus sequence typing of Candida africana from patients with vulvovaginal candidiasis in New Delhi, India

Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana

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