Isolation and molecular characterization of the hemagglutinin gene of H9N2 avian influenza viruses from poultry in Java, Indonesia

Nugroho, Christian Marco Hadi; Silaen, Otto Sahat Martua; Kurnia, Ryan Septa; Soejoedono, Retno Damajanti; Poetri, Okti Nadia; Soebandrio, Amin

doi:10.5455/javar.2021.h530

Cited by 8 publications

(14 citation statements)

References 37 publications

(38 reference statements)

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“…Based on the molecular analysis, the antigenic site at positions 125 S, 135 D, 147 K, 152 P, and 183N showed the same motif as Indonesia H9 LPAI viruses that had been previously reported in Yogyakarta and Central Java (Lestari et al 2019), in Sulawesi (Muflihanah et al 2017), Banten and North Sumatra (Jonas et al 2018;Nugroho et al 2021). The other important residues are the receptor binding site of A/Layer/Rhb-1/MHW/2017, which showed an amino acid composition: 92 P, 143 W, 145 T, 173 N, 180 V, 184 L, and 185 Y.…”

Section: Discussionsupporting

confidence: 73%

Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production

Maharani¹,

Susetya²,

Wibowo³

2022

HAYATI J Biosci

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Avian influenza virus H9N2 subtype is considered a low pathogenic strain that has been reported in Indonesia since late 2016. The outbreak has caused economic losses for farmers due to the sharp drop in egg production. The evidence of the existence of AIV H9N2 has been published, but very limited information on the prominent symptom and macroscopic lesion. This research was a retrospective study of suspected avian influenza H9 subtype, obtained from layer commercial farm with recorded characteristic symptoms. Specific trachea samples were collected and further processed to be isolated, propagated using embryonated chicken egg, and then extracted the RNA for molecular detection using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). A positive result was further detected in its H-9 gene with RT-PCR technique and sequencing methods. One of five samples showed positive for RT-qPCR with CT value 30.19. Sequence analysis confirmed that the sample with characteristic macroscopic lesion could be detected in the presence of the AIV H9 subtype. Phylogenetic tree analyses revealed that this virus belongs to the China-Vietnam- Indonesia (CVI) lineage.

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Section: Discussionsupporting

confidence: 73%

Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production

Maharani¹,

Susetya²,

Wibowo³

2022

HAYATI J Biosci

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“…The supernatant containing the virus was then stored at −20°C for further genetic confirmation and 500 TCID50 calculation. Genetic confirmation of the virus originating from the supernatant was extracted using the Geneaid Viral Nucleic Acid Extraction Kit II and reverse transcription–polymerase chain reaction (RT-PCR) was carried out on the gene encoding the fusion NDV protein (Fus) with the primer sequences forward 5’-ATGGGCTCCAGACCTTCTACCA-3”and reverse 5’-CTGCCACTGCTAGT TGTGAT AATC-3” [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of inhibitor activity of bacterial sialidase from Clostridium perfringens against Newcastle disease virus in the cell culture model using chicken embryo fibroblast

Kurnia¹,

Setiawaty²,

Natih³

et al. 2022

J Adv Vet Anim Res

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Objective: The Newcastle disease virus (NDV) is an infectious disease that causes very high eco¬nomic losses due to decreased livestock production and poultry deaths. The vaccine’s ineffec¬tiveness due to mutation of the genetic structure of the virus impacts obstacles in controlling the disease, especially in some endemic areas. This study aimed to provide an alternative treatment for NDV infection by observing the viral replication inhibitor activity of Clostridium perfringens sialidase in primary chicken embryo fibroblast (CEF) cells. Materials and Methods: The virus was adapted in CEF monolayer cells, then collected thrice using the freeze–thaw method and stored at −20°C for the next step in the challenge procedure. C. perfringens crude sialidase was obtained, but it was further purified via stepwise elution in ion exchange using Q Sepharose® Fast Flow and affinity chromatography with oxamic acid agarose. The purified sialidase was tested for its toxicity, ability to breakdown sialic acid, stopping viral replication, and how treated cells expressed their genes. Results: According to this study, purified C. perfringens sialidase at dosages of 187.5, 93.75, and 46.87 mU effectively hydrolyzes CEF cells’ sialic acid and significantly inhibits viral replication on the treated cells. However, sialidase dosages of 375 and 750 mU affected the viability of mono¬layer CEF cells. Interestingly, downregulation of toll-like receptor (TLR)3 and TLR7 (p < 0.05) in the sialidase-treated group indicates viral endocytosis failure. Conclusions: By stopping endocytosis and viral replication in host cells, sialidase from C. perfrin¬gens can be used as an alternative preventive treatment for NDV infection.

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“…1 ). These viruses are genetically closely related to the Y280 lineage of H9N2 AIV in China [ 41 42 43 ]. In addition, The H9N2 AIV was first identified in Russia in 2012 but was not defined genetically.…”

Section: History and Current Situation Of Y280 Lineage Of H9n2 Aiv In...mentioning

confidence: 99%

Current situation and control strategies of H9N2 avian influenza in South Korea

Sagong

Lee

et al. 2023

J Vet Sci

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The H9N2 avian influenza (AI) has become endemic in poultry in many countries since the 1990s, which has caused considerable economic losses in the poultry industry. Considering the long history of the low pathogenicity H9N2 AI in many countries, once H9N2 AI is introduced, it is more difficult to eradicate than high pathogenicity AI. Various preventive measures and strategies, including vaccination and active national surveillance, have been used to control the Y439 lineage of H9N2 AI in South Korea, but it took a long time for the H9N2 virus to disappear from the fields. By contrast, the novel Y280 lineage of H9N2 AI was introduced in June 2020 and has spread nationwide. This study reviews the history, genetic and pathogenic characteristics, and control strategies for Korean H9N2 AI. This review may provide some clues for establishing control strategies for endemic AIV and a newly introduced Y280 lineage of H9N2 AI in South Korea.

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Isolation and molecular characterization of the hemagglutinin gene of H9N2 avian influenza viruses from poultry in Java, Indonesia

Cited by 8 publications

References 37 publications

Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production

Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production

Evaluation of inhibitor activity of bacterial sialidase from Clostridium perfringens against Newcastle disease virus in the cell culture model using chicken embryo fibroblast

Current situation and control strategies of H9N2 avian influenza in South Korea

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