Isolation and molecular characterization of type I and type II feline coronavirus in Malaysia

Alazawy, Amer; Suri, Arshad Siti; Rahman, Omar Abdul; Mohd, Hair Bejo; Bande, Faruku; Sharif, Shukri; Azmi, T. I.

doi:10.1186/1743-422x-9-278

Cited by 29 publications

(17 citation statements)

References 14 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Type I FCoV is the dominant genotype in most countries, including Austria, Malaysia and China [19, 22, 32], while type II is less common and is mainly found in southeast Asian countries. Co-infection with types I and II were reported sporadically in Europe a decade ago and in southeast Asia in recent years but has not been reported in mainland China [20, 23, 25, 32].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and molecular characteristics of feline coronavirus in southwest China from 2017 to 2020

Zhou

Huang

et al. 2021

Journal of General Virology

View full text Add to dashboard Cite

Feline coronavirus (FCoV) is the causative agent of feline infectious peritonitis and diarrhoea in kittens worldwide. In this study, a total of 173 feline diarrhoeal faecal and ascetic samples were collected from 15 catteries and six veterinary hospitals in southwest China from 2017 to 2020. FCoV was detected in 80.35 % (139/173) of the samples using the RT-nPCR method; these included infections with 122 type I FCoV and 57 type II FCoV. Interestingly, 51 cases had co-infection with types I and II, the first such report in mainland China. To further analyse the genetic diversity of FCoV, we amplified 23 full-length spike (S) genes, including 18 type I and five type II FCoV. The type I FCoV and type II FCoV strains shared 85.5–98.7% and 97.4–98.9% nucleotide (nt) sequence identities between one another, respectively. The N-terminal domain (NTD) of 23 FCoV strains showed a high degree of variation (73.6–80.3 %). There was six type I FCoV strains with two amino acid insertions (159HL160) in the NTD. In addition, 18 strains of type I FCoV belonged to the Ie cluster, and five strains of type II FCoV were in the IIb cluster based on phylogenetic analysis. Notably, it was first time that two type I FCoV strains had recombination in the NTD, and the recombination regions was located 140–857 nt of the S gene. This study constitutes a systematic investigation of the current infection status and molecular characteristics of FCoV in southwest China.

show abstract

Section: Discussionmentioning

confidence: 99%

“…FCoV has a worldwide distribution [19–25]. Infections with FCoV are mostly type I FCoV, while type II FCoV occurs primarily in Southeast Asia [20, 22, 23].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characteristics of feline coronavirus in southwest China from 2017 to 2020

Zhou

Huang

et al. 2021

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Three-hundred ml of body effusion samples and suspensions of rectal swab samples from FIP-suspected cases were prepared as 10% (w/v) suspensions with sterile phosphate-buffered saline. The fluid was clarified by centrifugation at 1,000 g for 10 min, and the supernatant was collected [ 27 ]. Viral RNA was extracted using a viral RNA purification kit according to the manufacturer’s instructions (E.Z.N.A.…”

Section: Methodsmentioning

confidence: 99%

Comparative sequence analysis of the accessory and nucleocapsid genes of feline coronavirus strains isolated from cats diagnosed with effusive feline infectious peritonitis

et al. 2021

View full text Add to dashboard Cite

Feline infectious peritonitis (FIP) is a lethal infectious disease of domestic cats caused by feline coronavirus (FCoV) infection. Feline infectious peritonitis virus (FIPV) is a mutant type of FCoV that is characterized by causing fibrinous serositis with effusions in the pleural and abdominal cavities (wet form) and/or granulomatous-necrotizing inflammatory lesions in several organs (dry form). There have been numerous studies on FIP worldwide, whereas information about this disease in Thailand is still limited. Most studies involving molecular surveillance and evaluation of FCoV field strains have examined the genetic diversity of the spike and accessory ORF3c coding regions. Of these, the S gene is more divergent and is responsible for the two FCoV serotypes, while ORF3c harbors mutations that result either in early termination or destruction of the protein. In this study, we investigated the genetic diversity and genetic relationships among the current Thai and global FCoV strains in the accessory and nucleocapsid genes using a virus-specific PCR method. Comparative sequence analysis suggested that the Thai FCoV isolates were most closely related to strains reported in the Netherlands, the USA, and China. In the ORF3ab sequences, some Thai strains were more than 99% identical to the DF-2 prototype strain. Truncation of the 3a gene product was found in Thai FCoV strains of group 2. Amino acid deletions were observed in the N, ORF3c, and ORF7b proteins of Thai FCoV sequences. The accessory gene sequence divergence may be responsible for driving the periodic emergence and continued persistence of FCoVs in Thai domestic cat populations. Our findings provide updated information about the molecular characteristics of the accessory and nucleocapsid genes of FCoV strains in circulation that were not previously documented in this country. Supplementary information The online version contains supplementary material available at 10.1007/s00705-021-05188-7.

show abstract

“…The main distinction between them is associated with their differences in the S gene sequence and antibody neutralization properties ( Hohdatsu et al, 1991 ; Jaimes et al, 2020 ; Shiba et al, 2007 ; Tekes and Thiel, 2016 ). Serotype FCoV-I is 80-90% more prevalent than FCoV-II worldwide ( Amer et al, 2012 ; Kummrow et al, 2005 ; Myrrha et al, 2019 ). The emergence of serotype FCoV-II has involved recombination events between the S and M genes of FCoV-I and CCoV-II ( Herrewegh et al, 1998 ; Terada et al, 2014 ).…”

Section: Relevant Coronaviruses In Veterinary Medicinementioning

confidence: 99%

Clinical and molecular aspects of veterinary coronaviruses

et al. 2021

View full text Add to dashboard Cite

Coronaviruses are a large group of RNA viruses that infect a wide range of animal species. The replication strategy of coronaviruses involves recombination and mutation events that lead to the possibility of cross-species transmission. The high plasticity of the viral receptor due to a continuous modification of the host species habitat may be the cause of cross-species transmission that can turn into a threat to other species including the human population. The successive emergence of highly pathogenic coronaviruses such as the Severe Acute Respiratory Syndrome (SARS) in 2003, the Middle East Respiratory Syndrome Coronavirus in 2012, and the recent SARS-CoV-2 has incentivized a number of studies on the molecular basis of the coronavirus and its pathogenesis. The high degree of interrelatedness between humans and wild and domestic animals and the modification of animal habitats by human urbanization, has favored new viral spreads. Hence, knowledge on the main clinical signs of coronavirus infection in the different hosts and the distinctive molecular characteristics of each coronavirus is essential to prevent the emergence of new coronavirus diseases. The coronavirus infections routinely studied in veterinary medicine must be properly recognized and diagnosed not only to prevent animal disease but also to promote public health.

show abstract

Isolation and molecular characterization of type I and type II feline coronavirus in Malaysia

Cited by 29 publications

References 14 publications

Prevalence and molecular characteristics of feline coronavirus in southwest China from 2017 to 2020

Prevalence and molecular characteristics of feline coronavirus in southwest China from 2017 to 2020

Comparative sequence analysis of the accessory and nucleocapsid genes of feline coronavirus strains isolated from cats diagnosed with effusive feline infectious peritonitis

Clinical and molecular aspects of veterinary coronaviruses

Contact Info

Product

Resources

About