Isolation and molecular characterization of Pasteurella multocida isolates of buffaloes

Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes

“…A total of 16 isolates that were isolated from different sources (196 samples) were used in the study. The isolates were confirmed molecularly and also typed for capsular typing in earlier work by Sujatha et al (2018).…”

“…The 16 P. multocida isolates under study showed 14 Cap A and 2 Cap B isolates. It was found that the cap A isolates were observed from apparently healthy or carrier animals where as Cap B from clinical cases of Haemorrhagic septicaemia in earlier work by Sujatha et al (2018).…”

Phenotypic and Genotypic Analysis of Antibiogram of Pasteurella multocida Isolated from Pneumonic Sheep Lungs

“…From 195 samples, seven P. multocida were isolated as pure culture by following standard bacteriological procedures with specific modifications as described by Sujatha et al, 2018.…”

“…P. multocida specific PCR (PM-PCR) was done using a set of primers as reported by Townsend et al (1998). A 10µl reaction mixture was used as described by Sujatha et al, 2018 with PCR conditions initial denaturation 94°C for 3 min followed by 30 cycles of denaturation 94°C for 30 sec, annealing for 57°C for 40 sec, extension 72°C for 60 sec and final extension 72°C for 10 min. The amplified gene products were subjected to agarose gel electrophoresis using 1.5% agarose (Invitrogen, UK) and then visualised by UV gel documentation system ((Bio-Rad).…”

“…Distribution of the antibiotic resistance genes and their comparison with phenotypic resistance were shown in Table 3 and 4. The isolation and identification of Pasteurella multocida from primary cultures was done by observing standard cultural and biochemical properties of the P. multocida. The conventional procedures were supported by observing an amplified product of 460 bp in PCR that confirmed the occurrence of P. multocida in the isolated sample (Sujatha et al, 2018;Sarangi et al, 2014;Prabhakar et al, 2012;Ewers et al, 2006). Out of 195 samples 7pure cultures were obtained and were used for studying antibiogram pattern.…”