Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

Shao, Hongxia; Li, Jinzhi; Yuan, Huisha; Ji, Lingfei; Zhang, Jun; Jin, Wenjie; Qian, Kun; Ye, Jianqiang; Qin, Aijian

doi:10.3389/fvets.2021.669154

Cited by 4 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Domestic pigeons, as animals that are free-flying and habituated to humans, have the potential for both being viral reservoirs and for transmitting various pathogens between poultry facilities and wild birds. For example, cases of detection of viruses pathogenic to poultry in pigeons have been documented for CIAV, PPMV-1 and HPAIV subtype H5N1, among others [43][44][45][46]. Due to the paucity of data about the genetic diversity and distribution of coronaviruses, monitoring pigeons for potentially pathogenic factors is of undeniable importance.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Phylogenetic Analysis of Avian Coronaviruses in Domestic Pigeons (Columba livia domestica) in Poland between 2016 and 2020

2022

View full text Add to dashboard Cite

While disease control in racing pigeons and the potential role of pigeons as vectors transmitting viruses to poultry are of importance, there is still a paucity of data concerning the occurrence of coronaviruses in pigeons. In this study, 215 domestic pigeons were tested for the presence of coronaviral genetic material using the nested PCR method, which revealed 57 positive samples (26.51%). The difference in coronavirus prevalence between young and adult pigeons (34.34% and 19.83%, respectively) has been found statistically significant. In contrast, no statistically significant difference has been demonstrated between the prevalence in symptomatic and asymptomatic birds, leaving the influence of coronavirus presence on pigeon health uncertain. Phylogenetic analysis of the RdRp gene fragment allowed us to assign all the obtained strains to the Gammacoronavirus genus and Igacovirus subgenus. The phylogenetic tree plotted using the ML method revealed that those sequences formed a group most similar to pigeon coronavirus strains from China, Finland, and Poland, and to a single strain from a common starling from Poland, which suggests wide geographical distribution of the virus and its possible transmission between various species.

show abstract

Section: Discussionmentioning

confidence: 99%

Occurrence and Phylogenetic Analysis of Avian Coronaviruses in Domestic Pigeons (Columba livia domestica) in Poland between 2016 and 2020

2022

View full text Add to dashboard Cite

show abstract

“…Chicken infectious anemia (CIA) is a viral disease caused by the chicken infectious anemia virus (CIAV) and primarily affects young chicks. It is characterized by aplastic anemia, pale bone marrow, lymphoid tissue atrophy, and compromised immune function [ 1 , 2 ]. CIAV is a non-enveloped, icosahedral symmetrical virus particle belonging to the Anelloviridae family, with a diameter ranging from 25 nm to 26.5 nm and a genome size of approximately 2.3 kb [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Whole Genome Analysis of Chicken Infectious Anemia Virus in an Outbreak of Disease in Adult Layer Hens

Zeng,

Zhang,

Zhang

2023

Veterinary Sciences

View full text Add to dashboard Cite

Chicken infectious anemia (CIA) poses a significant threat to the chicken industry in China. Due to its non-specific symptoms, the disease is often overlooked. This study aimed to conduct a comprehensive analysis of the etiology and pathology of CIA in Guangxi Province, China. Three strains of the chicken infectious anemia virus (CIAV) were isolated from liver samples of diseased 20-week-old chickens. The complete genomes of these strains were sequenced, and experiments on specific pathogen-free (SPF) chicks revealed that the GX21121 strain exhibited high virulence. Histopathological examination of the deceased chickens showed liver cell necrosis, fibrous serous exudation, inflammatory cell infiltration, hemorrhage in liver tissues, and congestion in lung and renal tissues. Phylogenetic analysis of the genome revealed that the three strains had a close genetic relationship to the Heilongjiang wild-type strain (GenBank KY486144). The genetic evolution of their VP1 genes indicated that all three CIAV isolates belonged to genotype IIIc. In summary, this study demonstrated the genomic diversity of three CIAV strains in adult layer hens. The isolation and characterization of the GX21121 strain as a highly virulent isolate provide valuable information for further investigations into the etiology, molecular epidemiology, and viral evolution of CIAV.

show abstract

“…CIAV is the only member of the genus gyrovirus of the family Anelloviridae with 20-dihedral symmetry and no envelope ( Goryo et al, 1985 ; Natesan et al, 2006 ), and almost all of its ssDNA nucleic acids are 2,298 nt in length ( Ducatez et al, 2006 ). CIAV contains three overlapped ORFs, encoding three distinct viral proteins: major capsid and immunogenic protein (VP1, 51.6 kDa), non-structural scaffold protein (VP2, 24.0 kDa), and apoptin (VP3, 13.6 kDa) ( Noteborn et al, 1991 ; Shao et al, 2021 ). Chickens are the host of CIAV, and all ages are susceptible, mainly affecting chickens of 2–3 weeks.…”

Section: Introductionmentioning

confidence: 99%

A new rapid and sensitive method for detecting chicken infectious anemia virus

Kong

Yao

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Since the chicken infectious anemia virus (CIAV) was discovered in 1979, which has been reported as an economically significant and immunosuppressive poultry disease in the world. A novel clinical detection method for the prevention and control of CIAV in the poultry sector is urgently needed. Here, we established a real-time recombinase-aided amplification assay (RAA) for CIAV on-site with a rapid, highly sensitive, strongly specific, low-cost, and simple operational molecular diagnosis detection method. The primers and probe were developed using the CIAV VP2 gene sequence, which has a 117-bp specific band. This assay, which could be carried out at 41°C and completed in 30 min without cross-reactivity with other viruses, had the lowest detection limit of 10 copies of CIAV DNA molecules per reaction. Furthermore, the kappa value of this assay was 0.947, the sensitivity was 93.33%, and the specificity was 100% when compared to the real-time quantitative polymerase chain reaction assay (real-time qPCR). These results indicate that using a real-time RAA assay to detect CIAV on-site could be beneficial. In the future, the real-time RAA test may be a regular assay for the prevention and control of CIAV, as well as help the reduction of economic losses in the poultry business.

show abstract

Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

Cited by 4 publications

References 16 publications

Occurrence and Phylogenetic Analysis of Avian Coronaviruses in Domestic Pigeons (Columba livia domestica) in Poland between 2016 and 2020

Occurrence and Phylogenetic Analysis of Avian Coronaviruses in Domestic Pigeons (Columba livia domestica) in Poland between 2016 and 2020

Isolation, Identification, and Whole Genome Analysis of Chicken Infectious Anemia Virus in an Outbreak of Disease in Adult Layer Hens

A new rapid and sensitive method for detecting chicken infectious anemia virus

Contact Info

Product

Resources

About