Isolation, Identification, and Whole Genome Analysis of Chicken Infectious Anemia Virus in an Outbreak of Disease in Adult Layer Hens

Zeng, Yueyan; Zhang, Hui; Zhang, Huanrong

doi:10.3390/vetsci10070481

Cited by 1 publication

(1 citation statement)

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“…Owing to its nonspecific symptoms, this disease is frequently overlooked. Nonetheless, it is noteworthy that outbreaks of CIAV infection have been documented in 20-week-old laying hens, especially in instances of co-infection where immunosuppressive factors could be significant [12]. In vitro infection and proliferation experiments revealed that CIAV replicated in chicken embryos and lymphoblastic cell lines, but not in chicken embryo fibroblasts (CEFs), chicken kidney cells, or other primary cell types [13].…”

Section: Introductionmentioning

confidence: 99%

Synergy of Subgroup J Avian Leukosis Virus and Chicken Infectious Anemia Virus Enhances the Pathogenicity in Chickens

Xu,

Li,

Nie

et al. 2024

Microorganisms

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Subgroup J avian leukemia virus (ALV-J) and chicken infectious anemia virus (CIAV) are widely acknowledged as significant immunosuppressive pathogens that commonly co-infect chickens, causing substantial economic losses in the poultry industry. However, whether co-infection of ALV-J and CIAV have synergistic pathogenicity remains uncertain. To explore their synergistic pathogenesis, we established a co-infection model of ALV-J and CIAV in HD11 cells and specific-pathogen-free (SPF) chickens. We discovered that ALV-J and CIAV can synergistically promote the secretion of IL-6, IL-10, IFN-α, and IFN-γ and apoptosis in HD11 cells. In vivo, compared to the ALV-J and CIAV mono-infected group, the mortality increased significantly by 27% (20 to 47%) and 14% (33 to 47%) in the co-infected group, respectively. We also discovered that ALV-J and CIAV synergistically inhibited weight gain and exhibited more severe organ damage in co-infected chickens. Furthermore, we found that CIAV can promote the replication of ALV-J in HD11 cells and significantly enhance ALV-J viral load in blood and tissues of co-infected chickens, but ALV-J cannot promote the replication of CIAV. Moreover, by measuring the immune organ indexes and proportions of blood CD3+CD4+ and CD3+CD8+ lymphocytes, more serious instances of immunosuppression were observed in ALV-J and CIAV co-infected chickens than in mono-infected chickens. Taken together, our findings demonstrate that ALV-J and CIAV synergistically enhance pathogenicity and immunosuppression.

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Section: Introductionmentioning

confidence: 99%