Isolation and measurement of carbonic anhydrase isoenzymes in erythrocytes of dogs

Nishita, Toshiho; Kondo, Hidenori; Ishida, Sayaka; Ochiai, Hideharu; Asari, Masao

doi:10.2460/ajvr.2000.61.387

Cited by 13 publications

(14 citation statements)

References 21 publications

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“…Ruminants are an exception, as they express only the CA-II isozyme [3] and the concentration of CA-II in bovine erythrocytes is 14.22 mg/g Hb (unpublished data). Mean concentrations of CA-I and CA-II in erythrocytes from thoroughbreds, Beagles [12], dogs native to Japan [12] and humans [1] are 1.89 and 0.89 mg/g Hb, 3.21 and 1.63 mg/g Hb, 11.03 and 3.29 mg/g Hb, and 13.68 and 1.59 mg/g Hb, respectively. Among these animals there is a large difference in CA-I levels in erythrocytes; nevertheless, with the exception of cows, the difference in CA-II levels is small.…”

Section: Discussionmentioning

confidence: 99%

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Measurement of Erythrocyte Carbonic Anhydrase Isozymes (CA-I and CA-II) in Racehorses and Riding Horses

Nishita

Takahasi

Kasuya

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Equine carbonic anhydrase isozymes (CA-I and CA-II) were purified from erythrocytes by several column chromatography. Polyclonal anti-CA-I and anti-CA-II sera were produced in rabbits. Sensitive competitive enzyme-linked immunosorbent assays (ELISA) were established to determine the developmental changes in CA-I and CA-II levels in equine erythrocytes. Concentrations of CA-I and CA-II in erythrocytes from 150 clinically normal thoroughbreds (123 racehorses and 27 riding horses) were determined by ELISA. Mean (± SD) concentrations of CA-I and CA-II in racehorses were 1.70 ± 0.48 and 0.94 ± 0.13 mg/g hemoglobin (Hb), respectively. Mean concentrations of CA-I and CA-II in riding horses were 2.34 ± 0.52 and 0.76 ± 0.08 mg/g Hb, respectively. When the CA levels in racehorses and riding horses were compared, the CA-I level in riding horses was higher than that in racehorses (p=0.01). The CA-II level in racehorses was higher than that in riding horses (p=0.02). These data suggest that the levels of CA isozymes in erythrocytes of racehorses were influenced by chronic physical stress. The CA-I concentration in erythrocytes of 2-month-old horses was approximately 0.25 mg/g Hb. The CA-I level noticeably increased during the first year of life and approached normal adult levels by 2 years. The CA-II level decreased slightly with age, indicating different regulation of CA-I and CA-II expression during development. KEY WORDS: carbonic anhydrase, equine, ELISA, erythrocyte.

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Section: Discussionmentioning

confidence: 99%

“…As we previously described the purification process in detail [12], briefly blood (500 ml) from a clinically normal female thoroughbred (8 years old) was collected in a bottle containing 3.8% citric acid. Erythrocytes were washed by centrifugation with 0.15 M NaCl.…”

Section: Purification Of Ca Isozymesmentioning

confidence: 99%

Measurement of Erythrocyte Carbonic Anhydrase Isozymes (CA-I and CA-II) in Racehorses and Riding Horses

Nishita

Takahasi

Kasuya

et al. 2005

The Journal of Veterinary Medical Science

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“…The purification procedure for canine CA-I, II and III has been provided by the our previous reports, and we also previously raised antisera against purified canine CA-I, -II and -III isoenzymes [1,11,12]. The canine CAs antisera used in this study have also been recognized as to their immunological specificities [1].…”

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confidence: 97%

Immunohistolocalization of Carbonic Anhydrase Isoenzymes (CA-I, -II and -III) in Canine Epididymis

Ichihara

Ishikawa

Kaseda

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. The immunolocalization of the efferent duct and the epididymis in canine was firstly examined using an the immunohistochemical method with the canine carbonic anhydrase (CA) -I, CA-II and CA-III antisera. The efferent duct was immunonegative for all present canine CA antisera. However, some slender shaped epithelial cells in the head and body segments of the epididymal duct were intensely reacted to the CA-II antiserum. These results suggested that the CA-II might be controlled in the luminal environment in the head and body segments of the canine epididymis by the proton and bicarbonate balance for the maintenance of the spermatozoal stability and movement.

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“…Canine CA-I was isolated using the method of Nishita et al [16]. Briefly, the CA fraction was obtained from lysate of washed canine erythrocytes using the chloroform-ethanol denaturation method, which is used for the preparation of CA in a number of other mammals.…”

Section: Isolation Of Canine Ca-imentioning

confidence: 99%

Carbonic Anhydorase Isoenzyme I (CA-I) Concentration in Feces and Urine as a Temporary Marker of Occult Blood in Beagle Dogs

et al. 2007

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Isolation and measurement of carbonic anhydrase isoenzymes in erythrocytes of dogs

Cited by 13 publications

References 21 publications

Measurement of Erythrocyte Carbonic Anhydrase Isozymes (CA-I and CA-II) in Racehorses and Riding Horses

Measurement of Erythrocyte Carbonic Anhydrase Isozymes (CA-I and CA-II) in Racehorses and Riding Horses

Immunohistolocalization of Carbonic Anhydrase Isoenzymes (CA-I, -II and -III) in Canine Epididymis

Carbonic Anhydorase Isoenzyme I (CA-I) Concentration in Feces and Urine as a Temporary Marker of Occult Blood in Beagle Dogs

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