Isolation and mapping of microsatellite markers specific for the D genome of bread wheat

Abstract

The potential of Aegilops tauschii, the diploid progenitor of the D genome of wheat, as a source of microsatellite markers for hexaploid bread wheat was investigated. By screening lambda phage and plasmid libraries of Ae. tauschii genomic DNA, dinucleotide microsatellites containing GA and GT motifs were isolated and a total of 65 functional microsatellite markers were developed. All primer pairs that were functional in Ae. tauschii amplified well in hexaploid wheat. Fifty-five loci amplified by 48 primer sets were placed onto a genetic framework map of the reference population of the International Triticeae Mapping Initiative (ITMI) 'Opata 85' x 'W7984'. The majority of microsatellite markers could be assigned to the chromosomes of the D genome of wheat. The distribution of the markers along the chromosomes is random. Chromosomal location of 22 loci nonpolymorphic in the reference population was determined using nullitetrasomic lines of Triticum aestivum 'Chinese Spring'. The results of this study demonstrate the value of microsatellite markers isolated from Ae. tauschii for the study of bread wheat. The microsatellite markers developed improve the existing wheat microsatellite map and can be used in a wide range of genetic studies and breeding programs.

Interactions between Glu-1 and Glu-3 loci and associations of selected molecular markers with quality traits in winter wheat (Triticum aestivum L.) DH lines

“…SSR markers were selected from previously published SSR-based genetic maps for wheat (Pestsova et al 2000; Röder et al 1998; Somers et al 2004). Seven different sets of microsatellite primers were used: gwm (Röder et al 1998), gdm (Pestsova et al 2000), wmc (Wheat Microsatellite Consortium), and barc, cfa, cfd, and gpw (http://wheat.pw.usda.gov/GG2/index.shtml).…”

“…SSR markers were selected from previously published SSR-based genetic maps for wheat (Pestsova et al 2000; Röder et al 1998; Somers et al 2004). Seven different sets of microsatellite primers were used: gwm (Röder et al 1998), gdm (Pestsova et al 2000), wmc (Wheat Microsatellite Consortium), and barc, cfa, cfd, and gpw (http://wheat.pw.usda.gov/GG2/index.shtml). SSR markers were chosen so that they corresponded to linkage groups 1A, 1B, 1D, 5A, 5B, and 5D, which are known to contain QTLs and candidate genes for bread quality traits (Arbelbide and Bernardo 2006; Perretant et al 2000).…”

Identification and independent validation of a stable yield and thousand grain weight QTL on chromosome 6A of hexaploid wheat (Triticum aestivum L.)

“…A total of 285 primer sets from JIC/ psp [[44],[48]], IPK Gatersleben/ gwm/gdm [[49],[50]], Wheat Microsatellite Consortium/ wmc [[51]], Beltsville Agricultural Research Station/ barc [[52]] and INRA/ cfa/cfd [[53]] collections were polymorphic between Spark and Rialto. To ensure maximum genome coverage, 164 markers were selected for population mapping using published consensus maps [[21]] aiming for a marker density every 20 cM where possible.…”

Transferability and Polymorphism Between Group 7 Chromosome Specific Simple Sequence Repeat (SSR) Markers of Bread Wheat and Its Related Non-ProgenitorAegilopsSpecies

“…A total of 173 SSR markers from bread wheat (Guyomarc'h et al 2002;Pestsova, Ganal, and Roder 2000;Roder et al 1998;Somers, Isaac, and Edwards 2004) belonging to the group 7 chromosome were amplified on the genomic DNA of bread wheat cultivars and related non-progenitor species. The polymerase chain reaction (PCR) mixture (20 µl) consisted of 250 nM of each primer (Metabion, Hysel India Pvt., Ltd.), 0.2 mM of dNTPs (New England Biolab), 1.5 mM MgCl 2 (Biochem), 1 Unit Taq polymerase, and 100 ng of template DNA.…”