Isolation and lipid composition of spinach chloroplast envelope membranes

Poincelot, Raymond P.

doi:10.1016/0003-9861(73)90437-2

Cited by 74 publications

(45 citation statements)

References 36 publications

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“…Neutral lipids were resolved by one-dimensional TLC in hexane-diethyl ether-acetic acid (80:20:1). Procedures were as described previously (17).…”

Section: Plantmentioning

confidence: 99%

“…Compounds were identified by several spray reagents (17) and comparison with RF values obtained with lipid standards (Supelco, Inc.). For lipid analyses compound locations were revealed by iodine vapor and each lipid was carefully removed by scraping off the silica gel with a razor blade after the iodine was evaporated.…”

Section: Plantmentioning

confidence: 99%

“…The major acyl fatty acids of the chloroplast envelope membrane were palmitic (C16:0, 41 and 36%) and linolenic (C18:3, 29 and 40%) adds for spinach and sunflower; palmitic (77%) and steanc (C,8:0, 12%) acids for young maize; and palmitic (61%), stearic (14%), and linolenic (13%) acids for mature maize. The differences in lipid and acyl fatty acid compositions among these plants which vary in their rates of net photosynthesis were largely quantitative rather than qualitative.Methods for isolating chloroplast envelope membranes from plant species with slow rates of net photosynthesis have been developed in several laboratories (4,11,17,20). Recently I have also described the isolation of such membranes from plant species with fast rates of net photosynthesis (21).…”

mentioning

confidence: 99%

“…Methods for isolating chloroplast envelope membranes from plant species with slow rates of net photosynthesis have been developed in several laboratories (4,11,17,20). Recently I have also described the isolation of such membranes from plant species with fast rates of net photosynthesis (21).…”

mentioning

confidence: 99%

“…The lipid compositions were previously determined only with chloroplast envelope membranes from plant species with slow rates of net photosynthesis (4,12,17), hence comparative studies are incomplete. This paper describes differences among the lipid and acyl fatty acid compositions of spinach, sunflower, and maize chloroplast envelope membranes.…”

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confidence: 99%

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Lipid and Fatty Acid Composition of Chloroplast Envelope Membranes from Species with Differing Net Photosynthesis

Poincelot¹

1976

Plant Physiol.

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Lipid and fatty acid compositions were determined for chloroplast envelope membranes isolated from spinach (Spinacia oleracea L.), sunflower (flelianthus annuus L.), and maize (Zea mays L.) leaves. The lipid composition was simiar in sunflower, spinach, and undifferentiated maize chloroplast envelope membranes and different in maize mesophyll chloroplast envelope membranes. The predominant lipid constituents in all envelope membranes were monogalactosyldiglyceride (27 to 46%), digalactosyldiglyceride (18 to 33%), and phosphatidylcholine (7 to 30%). The fatty acid composition was also similar in sunflower and spinach chloroplast envelope membranes in comparison to those from maize. The major acyl fatty acids of the chloroplast envelope membrane were palmitic (C16:0, 41 and 36%) and linolenic (C18:3, 29 and 40%) adds for spinach and sunflower; palmitic (77%) and steanc (C,8:0, 12%) acids for young maize; and palmitic (61%), stearic (14%), and linolenic (13%) acids for mature maize. The differences in lipid and acyl fatty acid compositions among these plants which vary in their rates of net photosynthesis were largely quantitative rather than qualitative.Methods for isolating chloroplast envelope membranes from plant species with slow rates of net photosynthesis have been developed in several laboratories (4,11,17,20). Recently I have also described the isolation of such membranes from plant species with fast rates of net photosynthesis (21). I believe that differences in the biochemical properties of these envelope membranes might be related to variations in chloroplastic permeability and metabolite transport and hence these variations in biochemical properties could effect photosynthetic efficiency (21).Characterization of these membranes has shown some differences in their transport abilities (18,19,21), enzymic levels (21), and hexosamine contents (22). The lipid compositions were previously determined only with chloroplast envelope membranes from plant species with slow rates of net photosynthesis (4, 12, 17), hence comparative studies are incomplete. This paper describes differences among the lipid and acyl fatty acid compositions of spinach, sunflower, and maize chloroplast envelope membranes. These plant species were selected for investigation because of their known differences in rates of net photosynthesis at high irradiance in normal air. Chloroplasts. Intact mesophyll chloroplasts were isolated from 10-g batches of freshly harvested, rinsed leaves 4 to 6 weeks of age for preparation from spinach, sunflower, and mature maize (5,8, 25). Undifferentiated intact chloroplasts were prepared from 4-to 6-day-old leaves of young maize (15). MATERIALS AND METHODS PlantChloroplast Envelope Membranes. Complete envelope membranes were removed from intact spinach, sunflower, and maize chloroplasts by osmotic shock and purified on a three phase discontinuous sucrose gradient. Details of the isolation procedure were described previously (21). Complete envelope membrane fractions were pooled and diluted 2-fold w...

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“…Neutral lipids were resolved by one-dimensional TLC in hexane-diethyl ether-acetic acid (80:20:1). Procedures were as described previously (17).…”

Section: Plantmentioning

confidence: 99%

Section: Plantmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Lipid and Fatty Acid Composition of Chloroplast Envelope Membranes from Species with Differing Net Photosynthesis

Poincelot¹

1976

Plant Physiol.

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Changes in lipids, chlorophyll pigments, hot water insoluble n and ph of alfalfa during ensiling

et al. 1993

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This study examined (i) changes in the lipids of alfalfa (Medicago satiua) as a result of wilting and ensiling, and (ii) the breakdown of forage protein in direct cut and wilted forages following ensiling in relation to the remaining quantity of chlorophyll pigments a and b and forage pH. Lipid and malondialdehyde (MDA) were estimated in whole homogenates, chloroplast membranes and soluble fractions of freshly cut, wilted and ensiled alfalfa. Chlorophyll a and b, hot-waterinsoluble nitrogen (HWIN) and pH were also determined during the ensiling of freshly cut and 24 h wilted alfalfa herbages. Highest levels (P c 0.05) of lipid and MDA were observed in wilted alfalfa. The soluble fractions had the lowest levels of both lipids and MDA. Chlorophyll pigments showed an initial rapid decrease, followed by gradual decrease with time of ensiling. The rate of chlorophyll b disappearance in both direct ensiled and wilted ensiled forages was significantly (P < 0.05) correlated ( r = 0.81 and 0.86, respectively) to the breakdown rate of forage protein. Forage pH was also significantly (P <: 0.05) correlated to the forage protein breakdown (r = 0.59 and 0.61) for direct and wilted ensiled forages, respectively. Regression analyses of pH, chlorophyll pigments a and b versus forage HWIN showed that chlorophyll b was a better predictor of alfalfa HWIN quantity. These findings suggest that peroxidation of alfalfa chloroplast membrane lipids occurred during wilting and ensiling. Alfalfa chlorophyll pigments can potentially be employed to estimate alfalfa protein quantity during ensiling. Methods that arrest chloroplast disintegration such as forage blanching could increase ruminant utilization of alfalfa silages.

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GIycoconjugates

Robyt¹

1998

Springer Advanced Texts in Chemistry

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Isolation and lipid composition of spinach chloroplast envelope membranes

Cited by 74 publications

References 36 publications

Lipid and Fatty Acid Composition of Chloroplast Envelope Membranes from Species with Differing Net Photosynthesis

Lipid and Fatty Acid Composition of Chloroplast Envelope Membranes from Species with Differing Net Photosynthesis

Changes in lipids, chlorophyll pigments, hot water insoluble n and ph of alfalfa during ensiling

GIycoconjugates

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