Isolation and identification of Streptococcus pneumoniae serotype 6B from a patient with bacterial meningitis infection in Jakarta, Indonesia

Abstract

CNS infection is a life-threatening condition in developing countries and Streptococcus pneumoniae has been reported as the most common cause of bacterial meningitis; however, there is limited data on pneumococcal meningitis in Indonesia. This cross-sectional study aimed to isolate and identity S. pneumoniae strains from cerebrospinal fluid (CSF) specimens collected as part of routine testing from patients with clinically diagnosed central nervous system infection at a national referral hospital in Jakarta, Indonesia in 2017. S. pneumoniae isolation and identification were performed using conventional culture and molecular tools. Antibiotic susceptibility patterns were monitored through minimum inhibitory concentration testing. From 147 CSF specimens, one S. pneumoniae strain was identified from a patient with bacterial meningitis symptoms. The isolate was serotype 6B (ST5661) and susceptible to 18 antimicrobial agents tested, including penicillin, tetracycline, and the macrolide group. Our data provide insights into the epidemiology of invasive pneumococcal disease in Indonesia.

Bacterial Meningitis Among Adult Patients at University of Gondar Comprehensive Specialized Referral Hospital

“…In this study, ampicillin (100%), ceftriaxone (100%), chloramphenicol (77.80%), ciprofloxacin (83.30%), penicillin G (100%), vancomycin (87.50%), and erythromycin (80%) are the most effective antimicrobial agent for S. pneumoniae isolates. This finding was in agreement with studies conducted by Purwanto et al and by Gudina et al 17 , 33 However, a study by Assegu Fenta D et al 18 and Khan et al 24 reported that S. pneumoniae was resistant to ceftriaxone and penicillin. Cefoxitin (100%) and clindamycin (100%) are the most effective drugs for S. aureus isolates while erythromycin (66.80%) and amoxicillin (66.80%) are functional against S. aureus isolates.…”

“…The overall prevalence of culture-positive bacterial meningitis among adult patients in this study is 1.28%, which is higher than a study carried in Indonesia (0.68%). 17 However, it is lower than reported in Ethiopia (6.90%), 18 Malawi (5.25%), 19 South African (10.70%), 20 Kenya (11.20%), 21 Netherlands (13%), 22 Yemen (52.70%), 23 and Qatar (53.60%), 24 which differs noticeably among studies, might be due to differences in characteristics and geographical distribution of the study population, sample size, diagnostic techniques, and differences in infection control policies. We noticed a significant prevalence of bacterial meningitis among adult patients, which indicates adults remain the population where the disease meningitis prevalent and strict prevention strategies are required.…”

Bacterial Meningitis among Adult Patients at University of Gondar Comprehensive Specialized Referral Hospital: Prevalence and Antimicrobial Susceptibility Patterns

“…In this study, the majority of the isolated S. pneumonia were sensitive to ampicillin (100%), ceftriaxone (100%), chloramphenicol (77.8%), cipro oxacin (83.3%), penicillin G (100%), vancomycin (87.5%), and erythromycin (80%). This nding was in agreement with studies conducted by Purwanto DS et al [17] and by Gudina EK et al [33]. However, a study by Assegu Fenta D et al [18] and Khan FY et al [24] reported that S. pneumoniae was resistant to ceftriaxone and penicillin.…”

“…We performed this study to summarize prevalence gures obtained from CSF samples, with an exclusive focus on adults. The overall prevalence of bacterial meningitis among adult patients in this study is 1.28%, which is higher than a study carried in Indonesia (0.68%) [17]. However, it is lower than reported in Ethiopia (6.9%) [18], Malawi (5.25%) [19], South African (10.7%) [20], Kenya (11.2%) [21], Netherlands (13%) [22], Yemen (52.7%) [23] and Qatar (53.6%) [24], which differs noticeably among studies, might be due to differences in characteristics and geographical distribution of the study population, sample size, diagnostic techniques, and differences in infection control policies.…”

Determine the factors affecting the time to recovery of children with bacterial meningitis at Jigjiga university referral hospital in the Somali Regional State of Ethiopia: using the parametric shared frailty and AFT models

“…Meningitis causes significant neurosensory consequences and a high fatality rate (about 117,000 deaths annually globally). The disease’s median incidence in children under five is 34 cases per 100,000 children annually [ 6 ]. The incidence varies by global region; in the Americas, it is 16.6 per 100,000 children year, whereas in Africa, it is 143.6 per 100,000 children annually.…”