ISOLATION AND IDENTIFICATION OF Salmonella enterica TYPHIMURIUM FROM RABBITS

Abstract

The current study aimed to isolate and identify Salmonella Typhimurium from rabbits and evaluate their antimicrobial resistance. Seventy five New-Zealand breed rabbits of both sex (40 apparently healthy and 35 clinically diseased) were collected from Sharkia Province, Egypt. The collected samples from diseases cases (No=135) included raw meat, intestinal content (diarrheic), and liver (35 each). Vaginal swabs (No = 10) from aborted cases and abscess samples (No =20) were also collected. Isolation and identification of Salmonella enterica was carried out by standard methods. The isolates were characterized by using serological test, phagetyping, genus specific PCR and antimicrobial sensitivity tests. The overall prevalence of S. Typhimurium in the examined rabbit samples was 7.40% (10/135). S. Typhimurium was more frequently detected in vaginal swab (aborted cases) and diarrheic faeces with a frequency of 40% (4/10) and 14.29(5/35), respectively. Nevertheless, it was lower in the examined liver samples with a percentage of 5(1/35). S. Typhimurium was absent in apparently healthy rabbit samples. It was found that all S. Typhimurium isolates phage-typed as DT104. All PCR products of isolates include positive control, screened by PCR, resulted in 186 bp amplified fragment. No amplified DNA fragments were obtained from non-Salmonella species. Conclusively, it was concluded that rabbit is a potential reservoir for salmonellosis. The conservative Salmonella serotypes in the present study, was S. Typhimurium DT104. Multiresistance phenonmena was emergent in the circulating Salmonella strains in rabbit sources.

Survey of Salmonellae occurrence in meat‐producing rabbitries in Switzerland

“…The finding of 6% of farms positive for Salmonella Typhimurium in this study corresponds with the clinical occurrence of Salmonella of less than 8% reported in other studies. 2 , 3 , 4 , 7 , 8 , 9 The prevalence in Switzerland might be even lower because the three positive farms correspond to a single outbreak that was detected with clinical surveillance. Among rabbits showing clinical signs compatible with salmonellosis, approximately 4% were positive for Salmonella in Spain with around half of them being serotyped as Salmonella Typhimurium 3 – the serovar most often isolated in rabbits.…”

Chitosan propolis nanocomposite alone or in combination with apramycin: an alternative therapy for multidrug-resistant Salmonella Typhimurium in rabbits: in vitro and in vivo study

“…Other researchers from Egypt also used erythromycin, streptomycin, gentamycin, spiramycin, sulfamethoxazole, and tetracycline, as in our study, to determine the antimicrobial susceptibility of S. Typhimurium isolates from rabbits. They reported the following rates of sensitivity: 50, 60, 80, 90, 90 and 70%, respectively [31]. However, we obtained contrasting results, where the isolates showed high degrees of resistance to such antimicrobials, as shown in Fig.…”

Prevalence and antimicrobial sensitivity of Escherichia coli and Salmonella species in field cases of rabbit intestinal coccidiosis treated with prebiotic

“…Hamed et al (2013) reported hemorrhagic colitis and diarrhoea in newborn New Zealand rabbits infected with E. coli. Also, Salmonella enterica infection in rabbits is considered a potential risk associated with this animal species (Suelam and Reda 2015). Its occurrence among domestic rabbits is probably variable (Rodriguez-Calleja et al 2006).…”

“…According to Lim et al (2012), the prevalence of Salmonella species in rabbits ranged from 6 to 9%. It can occasionally cause a severe disease condition with a high mortality rate (Suelam and Reda 2015).…”