Isolation and Identification of Salmonella paratyphi from Enteric Fever Patients at Different Hospitals of Quetta City

Panezai, Malala; Taj, Muhammad Kamran; Nawaz, Imran; Taj, Imran; Panezai, Marina; Panezai, Nazia; Zafar, Umbreen; Muhammad, D.; Essote, Saeed Ahmed; Muhammad, Ghulam

doi:10.3923/pjbs.2018.469.474

Cited by 5 publications

(8 citation statements)

References 7 publications

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“…The S. paratyphi can be isolated from paratyphoid fever patients' blood for diagnosis. Paratyphoid fever is highest in teenagers and young adults as compared to typhoid fever that is common in children as being highlighted by [11].…”

Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

“…Nevertheless, the DNA of some bacterial isolates that contributed towards gastrointestinal ailments were identified on different percentages using the DNA purification kit (Hiper Bacterial Genomic DNA Extraction Teaching Kit) [11]. Consequently, a very reasonable number affected with S. paratyphi and paratyphoid out of reasonable number of the samples collected.…”

Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

“…PCR based identification of specific gene used to sense S. Paratyphi. All isolates of S. paratyphi used to produce the specific size of 329 base pair fragment of flic-a gene as carried out by [11].…”

Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

“…S. paratyphi transmission is through faecal oral route or via eating of unclean food/water as well as coming in contact with chronic asymptomatic carriers. The S. paratyphi caused enteric fever which is an important health issue in many developing countries [11].…”

Section: Introductionmentioning

confidence: 99%

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Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Abdallah

Mustafa

et al. 2019

ARRB

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Plants play vital roles in many health care systems, be it rural or an urban community. Plants became familiar as medicine due to the primordial ideologies and believed. Several plant parts served as medicines to so many ailments including gastrointestinal ailments, due to the fact that their active ingredients are powerful against the microbes as well as healing so many physiological abnormalities. The principal antimicrobial components were used to inhibit the growth of microbes (S. aureus, E. coli, Salmonella spp, B. cereus, and B. subtili,), as well as most of the recognized compounds in most plants were aromatic or saturated organic compounds which enabled the plants to be active against the gastrointestinal microbes. The commonly used diluents were; water, methanol and Di methyl sulphate oxides to ascertain the level of activity of the plants. As such, plant materials in one way or the other are very active when dealing with microbes due to their active ingredients or the phytoconstituents. Most of the microbes identified in many reviewed researches were enteric bacterial species, by which divided into both gram negative and gram positive bacterial isolates, they differ in their cell components, which are the main targets of bioactive constituents to deal with any bacteria. However, certain parasites contributed towards the production of ailments for their survival and causing havoc to the hosts and sometimes be mutualistic.

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Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

Section: Diversity Of Gastrointestinal Species and Their Activities On Different Plants Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Abdallah

Mustafa

et al. 2019

ARRB

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“…Subsequently, the Salmonella paratyphi is a Gram-negative, rod-shaped, facultative anaerobe, non-encapsulated, non-spore-forming, flagellated and motile bacterium. Three serotypes of S. paratyphi were described: S. paratyphi A, B, and C worldwide (Panezai et al, 2018). S. paratyphi transmission was through the fecal-oral route or via eating of unclean food/water and coming in contact with chronic asymptomatic carriers (Panezai et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Determination of Phenolics and Flavonoids of Some Useful Medicinal Plants and Bioassay-Guided Fractionation Substances of Sclerocarya birrea (A. Rich) Hochst Stem (Bark) Extract and Their Efficacy Against Salmonella typhi

et al. 2021

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Gallic acid and catechin are the most abundant phenolic and flavonoid contents found in all plant extracts. The contents and the bioassay-guided fractionating substances of the Sclerocarya birrea (A. Rich) Hochst (Anacardiaceae) fraction played vital roles. The goals of the study were to determine the contents of some useful medicinal plants and the bioassay-guided fractionation substances of S. birrea fraction compounds capable of acting against Salmonella isolate using LC-MS/LC-HRMS (Dionex ultimate 3000 RS UPLC with Thermo Scientific Q Exactive Orbitrap Hybrid Tandem Mass Spectrometer). The Folin–Ciocalteu reagent procedure and flavonoid content determination were conducted spectrophotometrically. Bioassay-guided fractionation, chronological partitioning, and screening of the antibacterial action against Salmonella typhi were performed. The ethyl acetate fraction extracts of S. birrea stem (bark) extract were analyzed using LC-MS/LC-HRMS. The gallic acid content increased tremendously in Vachellia nilotica (L.) P.J.H. Hurter and Mabb (Fabaceae) pod extracts with curve fitting (R2 = 0.9958). Catechin content increase was significantly increased in S. birrea stem (bark) extracts followed by that of V. nilotica pod extracts with curve fitting (R2 = 0.9993); they were all significantly different in the Guiera senegalensis J.F. Gmel. and the Leptadenia lanceolata (Poir.) Goyder leaves extracts at p value <0.0001. Subsequently, 10 mg/ml of S. birrea stem (bark) ethyl acetate fraction extract was the MIC, where no MBC was recorded and susceptible to the positive control with the highest inhibition zone, followed by the ethyl acetate fraction extract at 10 mg/ml (9.7 ± 0.0) at Turkey’s p < 0.0001. Vidarabine is one of the novel compounds, specifically having antimicrobial actions, found in the S. birrea stem (bark). Reasonable amounts of phenolic and flavonoid contents determined the actions of the individual plant extract.

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Reconnoitring Natural Antibacterial Appraisal of Medicinal Plants Extract Against Human Pathogen <i>Salmonella Paratyphi A</i> and <i>Salmonella Paratyphi B</i>

Ruparelia¹,

Rabari²,

Joshi³

et al. 2021

CBE

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Regardless of the convenience of plentiful miscellaneous collection of synthetic products and highthroughput tactics for their biological testing, natural compounds twig at a major source for antimicrobial drug development. These compounds are exclusively treasured as they have endured natural assortment over time. In this study we concentrated on Ethnobotanical efficacy of Indian medicinal plants like Ocimum sanctum, Phyllanthus emblica and Bryophyllum pinnatum for defence against bacterial human pathogen salmonella paratyphi A and salmonella paratyphi B. Phytochemical screening of these plants was executed for constituents like alkaloids, flavonoids, tannins, steroids, glycosides, carbohydrates and aminoacids. The ethanol extract of these plants exhibited good activity against the human pathogens by agar well diffusion assay method and the MIC was recorded. Average mean zone of inhibition found by these plants ranged between 4 mm to 29 mm and 4 mm to 27 mm respectively for salmonella paratyphi A and salmonella paratyphi B. These results accomplish the antimicrobial potential of the medicinal plants and hence convey upkeep for the use of them in traditional medicine.

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Isolation and Identification of Salmonella paratyphi from Enteric Fever Patients at Different Hospitals of Quetta City

Cited by 5 publications

References 7 publications

Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Determination of Phenolics and Flavonoids of Some Useful Medicinal Plants and Bioassay-Guided Fractionation Substances of Sclerocarya birrea (A. Rich) Hochst Stem (Bark) Extract and Their Efficacy Against Salmonella typhi

Reconnoitring Natural Antibacterial Appraisal of Medicinal Plants Extract Against Human Pathogen <i>Salmonella Paratyphi A</i> and <i>Salmonella Paratyphi B</i>

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Isolation and Identification of Salmonella paratyphi from Enteric Fever Patients at Different Hospitals of Quetta City

Cited by 5 publications

References 7 publications

Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Review on Some Plants’ Therapeutic Effects against Gastrointestinal Microbes

Determination of Phenolics and Flavonoids of Some Useful Medicinal Plants and Bioassay-Guided Fractionation Substances of Sclerocarya birrea (A. Rich) Hochst Stem (Bark) Extract and Their Efficacy Against Salmonella typhi

Reconnoitring Natural Antibacterial Appraisal of Medicinal Plants Extract Against Human Pathogen &lt;i&gt;Salmonella Paratyphi A&lt;/i&gt; and &lt;i&gt;Salmonella Paratyphi B&lt;/i&gt;

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Reconnoitring Natural Antibacterial Appraisal of Medicinal Plants Extract Against Human Pathogen <i>Salmonella Paratyphi A</i> and <i>Salmonella Paratyphi B</i>