Isolation and identification of probiotic lactic acid bacteria from curd and in vitro evaluation of its growth inhibition activities against pathogenic bacteria

Hawaz, Estifanos

doi:10.5897/ajmr2014.6639

Cited by 52 publications

(16 citation statements)

References 23 publications

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“…Several earlier reports have documented the varied antibacterial activity of CFSs of Lactobacillus strains. Similar to our findings, Hawaz (2014) had also observed that filtered supernatants of some of the tested Lactobacillus strains did not exhibit any inhibitory activity against Staphylococcus , E. coli , and Klebsiella . Recently, Jose et al (2015) reported that none of the lactobacilli supernatant could inhibit the growth of E. coli .…”

Section: Resultssupporting

confidence: 92%

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Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

et al. 2017

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This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei, L. delbrueckii, L. fermentum, L. plantarum, and L. pentosus. Antibacterial activities of cell-free supernatants (CFSs) of all the Lactobacillus isolates were estimated through standard agar-well diffusion assay, against commonly occurring food-borne and clinically important human pathogens. None of the lactobacilli cell-free supernatant (CFS) exhibited inhibitory activity against four pathogens, namely Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Klebsiella pneumoniae. Bacillus cereus, Salmonella enterica serovar Typhi, and Shigella flexneri were moderately inhibited by majority of CFSs, whereas, weak activity was observed against Pseudomonas aeruginosa and Proteus mirabilis. CFS of some of the curd isolates displayed antagonistic activity against Streptococcus mutans; however, human milk lactobacilli did not displayed any inhibitory activity against them. As expected, Nisin (Nisaplin®) showed inhibitory activity against Gram-positive, S. aureus, B. cereus, and L. monocytogenes. Interestingly, few of the examined CFSs exhibited inhibitory activities against both Gram-positive and Gram-negative pathogens. Findings from this study support the possibility to explore the tested lactobacilli and their CFSs as natural bio-preservatives, alone or in combination with approved bacteriocins in food and pharma formulations after validating their safety.

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Section: Resultssupporting

confidence: 92%

“…Our findings are in agreement with those obtained by Balamurugan et al (2014), who also reported high antagonistic activity of curd Lactobacillus isolates of Indian origin against Salmonella Typhimurium and E. coli . In another study, Hawaz (2014) also reported moderate activity of curd lactobacilli against Bacillus sp. and Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 92%

Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

et al. 2017

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“…Furthermore, in agreement with other studies, none of the isolated LAB showed hemolytic activity on sheep blood agar (Maragkoudakis et al, 2006;Hawaz, 2014). Resistance to gastric conditions and absence of hemolytic activity are important safety parameters for the selection of probiotic strains (FAO/WHO, 2002).…”

Section: Discussionsupporting

confidence: 88%

Isolation and characterization of vaginal Lactobacillus spp. in dromedary camels (Camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates

El-Deeb

Fayez²,

Elsohaby

et al. 2020

PeerJ

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Lactobacillus spp. is one of the beneficial lactic acid producing microbiota in the vagina, which is important for a healthy vaginal environment. However, little is known about vaginal Lactobacillus in dromedary camels (Camelus dromedarius). Therefore, this study aimed to isolate vaginal lactic acid bacteria (LAB) in dromedary camels and to study the probiotic potential of selected isolates. A total of 75 vaginal swabs were collected from pluriparous, non-pregnant, non-lactating dromedary camels. The LAB were isolated using deMan, Rogosa and Sharpe broth and agar media. Suspected LAB isolates were subjected to catalase testing and Gram staining and examined for indole production, nitrate reduction, hemolytic activity, cell surface hydrophobicity, auto- and coaggregation, antibacterial activity and characterized by 16S rRNA amplification and sequencing. Eighteen LABs were isolated from the 75 vaginal swabs. Among the 18 LAB isolates, six were Lactobacillus plantarum, eight were Lactobacillus fermentum, and four were Lactobacillus rhamnosus. None of the LAB isolates was hemolytic and only four LAB were H2O2 producing. The percentage of hydrophobicity ranged from 0% to 49.6%, 0% to 44.3% and 0% to 41.6% for hexadecane, xylene and toluene, respectively. All isolates showed higher (P < 0.05) autoaggregation after 24 h of incubation compared to 4 h. Furthermore, all LAB showed higher coaggregation (P < 0.05) and antimicrobial activity toward Staphylococcus aureus than to Escherichia coli. All LAB isolates were vancomycin resistant and sensitive to streptomycin, erythromycin, kanamycin and chloramphenicol. Only, three LAB isolates were resistant to tetracycline. The dromedary camel vaginal LAB isolates exhibited varying degrees of in vitro probiotic properties tested in this study and showed promising activity against the most common bacterial causes of endometritis in dromedary camels. Further investigation of the in vivo effect of these isolates is warranted.

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“…The ability to withstand bile salt is an important factor for the in vitro selection of probiotic bacteria [22, 36]. Both Lactobacillus salivarius C86 and Lactobacillus amylovorus C94 were able to survive simultaneous low pH and bile simulation at the pH of the stomach of cattle while it receives food [37].…”

Section: Discussionmentioning

confidence: 99%

Characterization and anti-salmonella activities of lactic acid bacteria isolated from cattle faeces

et al. 2018

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BackgroundNon typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics residue in animal products. One promising alternative to antibiotics in animal feed is Lactic Acid Bacteria (LAB) as probiotics. This study was carried out to determine the anti-salmonella activities and suitability of LAB isolated from cattle faeces in Nigeria as potential probiotics in cattle feed.MethodThe test Salmonella enterica spp strains and LAB were isolated from cattle faeces and identified by MALDI-TOF MS and partial sequencing of 16S rRNA genes respectively. The anti-salmonella activities of the isolated LAB in co-culture, cell-free supernatant, inhibition of growth by viable LAB cells and quantification of organic acids were determined by standard techniques. The ability of the LAB strains to withstand gastric conditions, antibiotic susceptibility and their haemolytic ability on blood agar were also determined.ResultsA total of 88 LAB belonging to 15 species were isolated and identified from cattle faeces. The most abundant species were Streptococcus infantarius (26), Enterococcus hirae (12), Lactobacillus amylovorus (10), Lactobacillus mucosae (10) and Lactobacillus ingluviei (9). Most of the LAB strains showed good anti-salmonella activities against the test Salmonella enterica spp. with 2 Lactobacillus strains; Lactobacillus amylovorus C94 and Lactobacillus salivarius C86 exhibiting remarkable anti-salmonella activities with total inhibition of Salmonella spp after 18 hours of co-incubation. The selected strains were able to survive simultaneous growth at pH 3 and 7% bile concentration and are non hemolytic.ConclusionThis study reports the vast diversity of culturable LAB in cattle faeces from Nigeria and their putative in-vitro antibacterial activity against Salmonella enterica spp isolated from cattle. Lactobacillus amylovorus C94 and Lactobacillus salivarius C86 demonstrated promising probiotic potentials in-vitro and will be further tested in-vivo in animal field trial.

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Isolation and identification of probiotic lactic acid bacteria from curd and in vitro evaluation of its growth inhibition activities against pathogenic bacteria

Cited by 52 publications

References 23 publications

Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

Isolation and characterization of vaginal Lactobacillus spp. in dromedary camels (Camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates

Characterization and anti-salmonella activities of lactic acid bacteria isolated from cattle faeces

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