Characterization and anti-salmonella activities of lactic acid bacteria isolated from cattle faeces

Abstract

BackgroundNon typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics residue in animal products. One promising alternative to antibiotics in animal feed is Lactic Acid Bacteria (LAB) as probiotics. This study was carried out to determine the anti-salmonella activities and suitability of LAB isolated from cattle faeces in Nigeria as potential probiotics in cattle feed.MethodThe test Salmonella enterica spp strains and LAB were isolated from cattle faeces and identified by MALDI-TOF MS and partial sequencing of 16S rRNA genes respectively. The anti-salmonella activities of the isolated LAB in co-culture, cell-free supernatant, inhibition of growth by viable LAB cells and quantification of organic acids were determined by standard techniques. The ability of the LAB strains to withstand gastric conditions, antibiotic susceptibility and their haemolytic ability on blood agar were also determined.ResultsA total of 88 LAB belonging to 15 species were isolated and identified from cattle faeces. The most abundant species were Streptococcus infantarius (26), Enterococcus hirae (12), Lactobacillus amylovorus (10), Lactobacillus mucosae (10) and Lactobacillus ingluviei (9). Most of the LAB strains showed good anti-salmonella activities against the test Salmonella enterica spp. with 2 Lactobacillus strains; Lactobacillus amylovorus C94 and Lactobacillus salivarius C86 exhibiting remarkable anti-salmonella activities with total inhibition of Salmonella spp after 18 hours of co-incubation. The selected strains were able to survive simultaneous growth at pH 3 and 7% bile concentration and are non hemolytic.ConclusionThis study reports the vast diversity of culturable LAB in cattle faeces from Nigeria and their putative in-vitro antibacterial activity against Salmonella enterica spp isolated from cattle. Lactobacillus amylovorus C94 and Lactobacillus salivarius C86 demonstrated promising probiotic potentials in-vitro and will be further tested in-vivo in animal field trial.

Protective Effect of Potential Probiotic Strains from Fermented Ethiopian Food against Salmonella Typhimurium DT104 in Mice

“…e highest inhibition (100%; p < 0.05) was observed with the combination of the three probiotic strains as in the form of mix 4 (Lactococcus lactis, Lactobacillus plantarum, and Lactobacillus paracasei). In agreement with this study, Adetoye et al [32] have revealed that Lactobacillus salivarius C86 and Lactobacillus amylovorus C94 strains obtained from cattle feces were able to inhibit the growth of Salmonella spp. completely between 8 and 16 hours of coincubation with no recoverable Salmonella spp.…”

Study of the antibiotic residues in poultry meat in some of the EU countries and selection of the best compositions of lactic acid bacteria and essential oils against Salmonella enterica

“…(2019) published that culture supernatants from Lactobacillus ingluviei strain UMNPBX19 and Lactobacillus salivarius strain UMNPBX2 exhibited antimicrobial activity against Salmonella . A study by Adetoye et al. (2018) demonstrated in vitro suppression of Salmonella by intestinal LAB from cattle ( Lactobacillus amylovorus C94 and L. salivarius C86).…”

Linking perturbations to temporal changes in diversity, stability, and compositions of neonatal calf gut microbiota: prediction of diarrhea

“…infantarius and S . equines in bovine fecal samples, exhibited anti- Salmonella activities [ 59 ]. Our results highlight the potential of using these butyrate-producing bacteria as microbial markers for identifying healthy (high prevalence) or unhealthy (low prevalence) in young ruminants.…”