Isolation and identification of pathogenic fungi causing postharvest fruit rot of kiwifruit (<i>Actinidia chinensis</i>) in China

Li, Li; Pan, Hui; Chen, Meiyan; Sheng-ju, Zhang; Zhong, Caihong

doi:10.1111/jph.12618

Cited by 47 publications

(39 citation statements)

References 22 publications

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“…Phomopsis sp. may cause leaf spot, fruit rot and postharvest fruit rot of kiwifruit [25,26]. Phytophthora capsici can infect various growth stages of pepper plants, causing seedling death, root rot, crown rot, stem blight, leaf spot and fruit rot [27,28].…”

Section: Discussionmentioning

confidence: 99%

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Shi

Wang

et al. 2019

Molecules

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Background: The development of new antifungal agents has always been a hot research topic in pesticide development. In this study, a series of derivatives of natural compound β-pinene were prepared, and the antifungal activities of these derivatives were evaluated. The purpose of this work is to develop some novel molecules as promising new fungicides. Methods: Through a variety of chemical reactions, β-pinene was transformed into a series of β-pinene-based derivatives containing amide moieties and acylthiourea moieties. The antifungal activities of these derivatives against five plant pathogens including Colletotrichum gloeosporioides, Fusarium proliferatum, Alternaria kikuchiana, Phomopsis sp. and Phytophthora capsici were tested; preliminary structure–activity relationship was discussed. Results: Some derivatives exhibited moderate or significant antifungal activity due to the fusion of the amide moiety or the acylthiourea moiety with the pinane skeleton. The structure–activity relationship analysis showed that the fluorine atom and the strong electron withdrawing nitro group, or trifluoromethyl group on the benzene ring of the derivatives had a significant effect on the improvement of the antifungal activity against Colletotrichum gloeosporioides, Fusarium proliferatum, Alternaria kikuchiana and Phomopsis sp. Meanwhile, the introduction of an ethyl group at the meta-position on the benzene ring of the derivatives could improve the antifungal activity against Phytophthora capsici. Compounds 4e, 4h, 4q, 4r exhibited broad-spectrum antifungal activity against the tested strains. Compound 4o had significant antifungal activity against Phytophthora capsici (IC50 = 0.18 μmol/L). These derivatives were expected to be used as precursor molecules for novel pesticide development in further research.

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Section: Discussionmentioning

confidence: 99%

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Shi

Wang

et al. 2019

Molecules

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“…Kiwifruit is an economically important fruit crop mainly grown in China, New Zealand, and Italy [1]. Ripe rot, caused by Botryosphaeria dothidea, is presently one of the most devastating diseases of kiwifruit in China and abroad, which restricts the sustainable development of kiwifruit industry [2][3][4]. In severe cases, the disease can cause up to 80% loss in production as occurred during 2011-2012 in Fengxin County, Jiangxi Province, China [5].…”

Section: Introductionmentioning

confidence: 99%

“…Fruit infection occurs mostly at the early fruiting stage. However, symptoms on fruit appear only from near maturity to storage, resulting in fruit drop and postharvest decay [2]. As B. dothidea is capable of infecting a large number of plant species and has latent infection features, developing kiwifruit varieties resistant to ripe rot through conventional breeding and biotechnology is considered one of the most effective management strategies.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection

Wang

Xiong

et al. 2020

PLoS ONE

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Ripe rot caused by Botryosphaeria dothidea causes extensive production losses in kiwifruit (Actinidia chinensis Planch.). Our previous study showed that kiwifruit variety "Jinyan" is resistant to B. dothidea while "Hongyang" is susceptible. For a comparative analysis of the response of these varieties to B. dothidea infection, we performed a transcriptome analysis by RNA sequencing. A total of 305.24 Gb of clean bases were generated from 36 libraries of which 175.76 Gb was from the resistant variety and 129.48 Gb from the susceptible variety. From the libraries generated, we identified 44,656 genes including 39,041 reference genes, 5,615 novel transcripts, and 13,898 differentially expressed genes (DEGs). Among these, 2,373 potentially defense-related genes linked to calcium signaling, mitogen-activated protein kinase (MAPK), cell wall modification, phytoalexin synthesis, transcription factors, pattern-recognition receptors, and pathogenesis-related proteins may regulate kiwifruit resistance to B. dothidea. DEGs involved in calcium signaling, MAPK, and cell wall modification in the resistant variety were induced at an earlier stage and at higher levels compared with the susceptible variety. Thirty DEGs involved in plant defense response were strongly induced in the resistant variety at all three time points. This study allowed the first comprehensive understanding of kiwifruit transcriptome in response to B. dothidea and may help identify key genes required for ripe rot resistance in kiwifruit.

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“…& De Not., Botrytis cinerea Pers., and Botryotinia fuckeliana (Karakaya & Bayraktar, 2009; Koh et al., 2003). B. dothidea is able to penetrate kiwifruits through both cell walls and wounds, while the other pathogens can enter only through wounds (Li et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

“…B. dothidea is able to penetrate kiwifruits through both cell walls and wounds, while the other pathogens can enter only through wounds (Li et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Improvement of antifungal activity of a culture filtrate of endophyticBacillus amyloliquefaciensisolated from kiwifruit and its effect on postharvest quality of kiwifruit

et al. 2020

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This study reports the optimization of culture conditions and medium components of M9, an endophytic strain of Bacillus amyloliquefaciens, which we had previously isolated from kiwifruit, and evaluation of its effect on kiwifruit postharvest protection against soft rot. The method of one-factor-at-a-time was used to determine the optimum culture conditions, and response surface methodology was applied to optimize the medium constituents. After optimization, a high rate of antifungal activity (73.12% decrease in decay rate) by M9 culture filtrate against the soft rot fungal pathogen, Botryosphaeria dothidea, was obtained. Compared with the initial culture conditions, the antifungal activity of M9 culture filtrate was increased by 19.5%. Furthermore, a postharvest storage experiment on kiwifruit showed that M9 culture filtrate could maintain the quality of stored kiwifruit, delay fruit senescence, and significantly enhance the storability of kiwifruit. Using liquid chromatography-mass spectrometry, we found that the antifungal activity of M9 was associated with the presence of the C12-surfactin A lipopeptide. Practical applications Soft rot caused by Botryosphaeria dothidea is one of the most important diseases of kiwifruit, causing postharvest fruit rot. The disease progresses rapidly and is difficult to control, posing a great threat to the kiwifruit industry. In this study, the culture conditions and medium composition for culture of the strain M9 (kiwifruit endophytic Bacillus amyloliquefaciens) were optimized to maximize the inhibition of B. dothidea. A postharvest storage experiment showed that M9 culture filtrate could improve the disease resistance of kiwifruits, delay the senescence of the fruits, and maintain the quality of kiwifruit during storage. Because M9 is a natural endophyte of kiwifruit, the strategy used in this study was both effective and safe. This work will contribute to the exploitation of B. amyloliquefaciens in controlling the pathogens of kiwifruit and the development of safer and more advanced strategies for the postharvest protection of kiwifruits.

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Isolation and identification of pathogenic fungi causing postharvest fruit rot of kiwifruit (Actinidia chinensis) in China

Cited by 47 publications

References 22 publications

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Derivatization of Natural Compound β-Pinene Enhances Its In Vitro Antifungal Activity against Plant Pathogens

Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection

Improvement of antifungal activity of a culture filtrate of endophyticBacillus amyloliquefaciensisolated from kiwifruit and its effect on postharvest quality of kiwifruit

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