Isolation and Identification of Major Pathogenic Bacteria from Clinical Mastitic Cows in Asella Town, Ethiopia

Abstract

Mastitis is a multietiological and complex disease causing inflammation of the parenchyma of mammary glands and is a problem in many dairy cows. The objective of this study was to isolate and identify the pathogenic bacteria that cause bovine clinical mastitis. A cross-sectional study was undertaken between November 2018 to April 2019 on a small scale and government dairy farms in Asella town. Cow’s udder and teats were physically examined to detect clinical mastitis. A total of 83 milk samples were collected from 46 cows that show clinical sign of mastitis from a total of 12 farms. Isolation and identification of major bacterial species were carried out by culturing different media and using primary and secondary biochemical tests. Out of the 83 samples collected and examined, all (100%) were positive for the cultural isolation of bacterial species. The bacteria were identified to genus and species level. Among the 83 isolates, 32 (38.6%), 24 (28.9%), and 6 (7.2%) were Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus hyicus, respectively. Other bacteria like Escherichia coli 12 (14.5%) and Streptococcus species 2 (2.4%) were also isolated. Bacillus species 2 (2.4%), Proteus species 2 (2.4%), and 3 (3.6%) of them were mixed bacterial infections. The present study revealed that both contagious and environmental bacterial pathogens were responsible for the occurrence of clinical mastitis. Proper milking practices and farm husbandry practices and future detailed studies up to the species level and on antibiotic profiles of the pathogens are needed.

Frequently used therapeutic antimicrobials and their resistance patterns on Staphylococcus aureus and Escherichia coli in mastitis affected lactating cows

“…The isolation rate was closely similar to the studies conducted in Italy (41%) [ 29 ] and slightly higher in Brazil (56%) [ 30 ]. Among S. aureus , 36.96% was found to be coagulase positive, which was closely similar to the previous findings [ 31 ]. These coagulase positive S. aureus strains are most frequently associated with contagious mastitis of bovine, caprine and ovine species due to the harbouring of multiple virulence factors such as Staphylococcal Protein-A, the coagulase enzyme, clumping factors, haemolysins, proteases and gelatinases [ 13 , 23 , 32 ].…”

Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils

“…P. mirabilis have been described as the causal agent of bovine mastitis [ 7 , 35 , 36 , 37 ]. Our results are similar to the study conducted by Parmar et al [ 35 ], who isolated two Proteus spp.…”

Rumen Microbiota Alterations During Ketosis is Associated with the Development of Mastitis in Dairy Cows

“…Mastitis is a multietiological and complex disease, which is de ned as in ammation of mammary glands [24]. It is recognized that mastitis mainly resulted from infection of pathogenic microorganisms.…”