Isolation and Identification of <i>Vibrio</i> sp. in the Hepatopancreas of Cultured White Pacific Shrimp (<i>Litopenaeus vannamei</i>)

Abstract

ABSTRACT. The Vibrio is the most common genera with crustaceans often causing various diseases in Aquaculture and significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was carried out, the isolation and identification of pathogenic bacterial flora were isolated from infected in hepatopancreas of vannamei. The SPDS Oceanic farm, RS Aqua farm of and Valli vilas Aqua farm Vellar estuary, Cuddalore District, Tamil Nadu, during the period of (September 2013 to November 2013). The collected samples were plated on TCBS-(Thiosulfate-Citrate-Bile salt-Sucrose) agar medium. The present study, totally 253 green colonies were isolated from TCBS agar plates and among these, 175colonies were identified by using the biochemical tests showed the V. parahaemolyticus, V. mimicus, V. vulnificus, V. damsela and P. shigelloides. The maximum species was recorded in V. parahaemolyticus (83.4 %) and minimum was observed in V. mimicus (1.7 %).

Phenotypic and genomic characterization of a Vibrio parahaemolyticus strain causing disease in Penaeus vannamei provides insights into its niche adaptation and pathogenic mechanism

“…Recently, V. parahaemolyticus has been increasingly reported to cause diseases in P. vannamei [8], of which acute hepatopancreatic necrosis disease (AHPND) is the most severe disease currently affecting shrimp aquaculture [9]. Its causative agent is commonly considered as a V.…”

“…The virulence of V. parahaemolyticus is variable depending on the virulence determinants, such as thermostable direct haemolysin (TDH), TDH-related haemolysin (TRH), thermolabile haemolysin (TLH), secretion systems, secretory proteins, prophage, plasmid and other regulatory virulence factors [ 6, 7 ]. Recently, V. parahaemolyticus has been increasingly reported to cause diseases in P. vannamei [ 8 ], of which acute hepatopancreatic necrosis disease (AHPND) is the most severe disease currently affecting shrimp aquaculture [ 9 ]. Its causative agent is commonly considered as a V. parahaemolyticus strain harbouring a 70 kb conjugative plasmid that carries pirA and pirB genes encoding the binary Photorhabdus insect-related toxins A and B (PirAB).…”

Dynamic stock model for analysing semi‐intensive production of whiteleg shrimpLitopenaeus(Penaeus)vannameiaffected by the acute hepatopancreatic necrosis disease: Assessment of disease severity indicators and relationships with pond water quality parameters

“…Similar to other aquaculture farms, the TCBS method used in this study was for monitoring yellow and green bacteria (Cadiz, Traifalgar, Sanares, Andrino‐Felarca, & Corre, ), but no correlations were found between the indicators of disease severity and the amounts of bacterial populations. Despite V. parahaemolitycus can be detected as a green colony with the TSBC method (Hasrimi, Budiharjo, & Jannah, ), these results might be a consequence of other Vibrio and not Vibrio species that can also be identified as green colonies with this method (Pfeffer & Oliver, ; Shanmugasundaram, Mayavu, Manikandarajan, Suriya, & Anbarasu, ), rendering the test inaccurate for detection of V. parahaemolitycus .…”