Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi

Fikri, Ahmad Syairazie Ibrahim; Rahman, Irman Abdul; Nor, Norefrina Shafinaz Md; Hamzah, Ainon

doi:10.1063/1.5027987

Cited by 12 publications

(8 citation statements)

References 11 publications

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“…Similarly, Iranian medicinal plants-endophytic actinomyctes exhibited antimicrobial activity against the pathogenic bacteria B. cereus, B. subtilis, E. coli, Citrobacter freundii, K . pneumoniae, Proteus mirabilis, Shigella flexneri , and S. aureus (Beiranvand et al, 2017; Figure 1, 3), whereas Malaysian plants-endophytic B. subtilis possessed antibacterial activity against S. aureus , methicillin resistant S. aureus , and P. aeruginosa (Fikri et al, 2018; Figure 2B). The African Combretum molle -endophytic Bacillus and Lysinibacillus species exhibited antibacterial activity against B. cereus, E. coli, P. aeruginosa , and S. aureus (Diale et al, 2018; Supplementary Table S1).…”

Section: Gram-positive Endophytes Against Human/animal Pathogensmentioning

confidence: 99%

Bioactive Products From Plant-Endophytic Gram-Positive Bacteria

et al. 2019

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Endophytes constitute plant-colonizing microorganisms in a mutualistic symbiosis relationship. They are found in most ecosystems reducing plant crops’ biotic and abiotic stressors by stimulating immune responses, excluding plant pathogens by niche competition, and participating in antioxidant activities and phenylpropanoid metabolism, whose activation produces plant defense, structural support, and survival molecules. In fact, metabolomic studies have demonstrated that endophyte genes associated to specific metabolites are involved in plant growth promotion (PGP) by stimulating plant hormones production such as auxins and gibberellins or as plant protective agents against microbial pathogens, cancer, and insect pests, but eco-friendly and eco-safe. A number of metabolites of Gram-positive endophytes isolated from agriculture, forest, mangrove, and medicinal plants, mainly related to the Firmicutes phyla, possess distinctive biocontrol and plant growth-promoting activities. In general, Actinobacteria and Bacillus endophytes produce aromatic compounds, lipopeptides, plant hormones, polysaccharides, and several enzymes linked to phenylpropanoid metabolism, thus representing high potential for PGP and crop management strategies. Furthermore, Actinobacteria have been shown to produce metabolites with antimicrobial and antitumor activities, useful in agriculture, medicine, and veterinary areas. The great endophytes diversity, their metabolites production, and their adaptation to stress conditions make them a suitable and unlimited source of novel metabolites, whose application could reduce agrochemicals usage in food and drugs production.

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Section: Gram-positive Endophytes Against Human/animal Pathogensmentioning

confidence: 99%

Bioactive Products From Plant-Endophytic Gram-Positive Bacteria

et al. 2019

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“…It assumes that the source of the beneficial/probiotic microorganisms such as natural, healthy habitats, unchanged by synthetic chemical treatment (or their natural equivalents) may bring about the expected functionality of the final bioproducts in terms of quality. As previously stated, the microbial strains obtained from such environments show activity that supports the growth and functioning of particular plants or/and manifest antagonistic properties [23,24]. For planted raspberries, the corresponding natural environment includes wild raspberry plants [2].…”

Section: Introductionmentioning

confidence: 93%

“…This approach was dictated by the current research which shows that strains of Trichoderma spp. differ greatly in their effects on plants [29], the locally isolated microorganisms might be more effective against local pathogens than bacteria from different regions of the world [23]. Thus, candidates from the relevant niche should be preferred [30].…”

Section: Fungal Consortium Selection For Biopreparationmentioning

confidence: 99%

Trichoderma-Based Biopreparation with Prebiotics Supplementation for the Naturalization of Raspberry Plant Rhizosphere

Oszust

Pylak

Frąc

2021

IJMS

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The number of raspberry plants dying from a sudden outbreak of gray mold, verticillium wilt, anthracnosis, and phytophthora infection has increased in recent times, leading to crop failure. The plants suffer tissue collapse and black roots, symptoms similar to a Botrytis–Verticillium–Colletotrichum–Phytophthora disease complex. A sizeable number of fungal isolates were acquired from the root and rhizosphere samples of wild raspberries from different locations. Subsequent in vitro tests revealed that a core consortium of 11 isolates of selected Trichoderma spp. was the most essential element for reducing in phytopathogen expansion. For this purpose, isolates were characterized by the efficiency of their antagonistic properties against Botrytis, Verticillium, Colletotrichum and Phytophthora isolates and with hydrolytic properties accelerating the decomposition of organic matter in the soil and thus making nutrients available to plants. Prebiotic additive supplementation with a mixture of adonitol, arabitol, erythritol, mannitol, sorbitol, and adenosine was proven in a laboratory experiment to be efficient in stimulating the growth of Trichoderma isolates. Through an in vivo pathosystem experiment, different raspberry naturalization-protection strategies (root inoculations and watering with native Trichoderma isolates, applied separately or simultaneously) were tested under controlled phytotron conditions. The experimental application of phytopathogens attenuated raspberry plant and soil properties, while Trichoderma consortium incorporation exhibited a certain trend of improving these features in terms of a short-term response, depending on the pathosystem and naturalization strategy. What is more, a laboratory-scale development of a biopreparation for the naturalization of the raspberry rhizosphere based on the Trichoderma consortium was proposed in the context of two application scenarios. The first was a ready-to-use formulation to be introduced while planting (pellets, gel). The second was a variant to be applied with naturalizing watering (soluble powder).

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“…Current research shows that locally isolated bacteria might be more effective against local pathogens than bacteria from different regions of the world [ 12 ]. Moreover, each plant species likely benefits from recruiting a specialized consortium of bacteria, which is specific for each plant system.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, the level of isolates survival and activity properties may be raised and influenced by different soil pH values or salinity levels in different ecological niches which they inhabit [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. The very first and preliminary research suggesting prebiotic’ additives for the future application into microbial-based biopreparations was published [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization

Pylak

Oszust

Frąc

2020

IJMS

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The threat caused by plants fungal and fungal-like pathogens is a serious problem in the organic farming of soft fruits. The European Commission regulations prohibit some commercially available chemical plant protection products, and instead recommend the use of natural methods for improving the microbial soil status and thus increasing resistance to biotic stresses caused by phytopathogens. The solution to this problem may be biopreparations based on, e.g., bacteria, especially those isolated from native local environments. To select proper bacterial candidates for biopreparation, research was provided to preliminarily ensure that those isolates are able not only to inhibit the growth of pathogens, but also to be metabolically effective. In the presented research sixty-five isolates were acquired and identified. Potentially pathogenic isolates were excluded from further research, and beneficial bacterial isolates were tested against the following plant pathogens: Botrytis spp., Colletotrichum spp., Phytophthora spp., and Verticillium spp. The eight most effective antagonists belonging to Arthrobacter, Bacillus, Pseudomonas, and Rhodococcus genera were subjected to metabolic and enzymatic analyses and a resistance to chemical stress survey, indicating to their potential as components of biopreparations for agroecology.

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Isolation and identification of local bacteria endophyte and screening of its antimicrobial property against pathogenic bacteria and fungi

Cited by 12 publications

References 11 publications

Bioactive Products From Plant-Endophytic Gram-Positive Bacteria

Bioactive Products From Plant-Endophytic Gram-Positive Bacteria

Trichoderma-Based Biopreparation with Prebiotics Supplementation for the Naturalization of Raspberry Plant Rhizosphere

Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization

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