Isolation and Identification of Klebsiella pneumoniae from Sheep-Case Report

Patel, Shraddha; Chauhan, H.C.; Patel, AC; Shrimali, M.D.; Patel, Kamlesh; Prajapati, Bhumika; Kala, JK; Patel, MG; Rajgor, Manish; Patel, M.A.

doi:10.20546/ijcmas.2017.605.037

Cited by 22 publications

(20 citation statements)

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“…Biochemical testing of the bacterial isolates produced positive results with Voges-Proskauer, Simmons' citrate, and sugar fermentation tests, but negative reactions were identified by Indol, methyl red, and motility tests. The similar results have also been reported by Patel et al, (2017) and Abdullah and Zghair (2016) [13], [14]. Accordingly, these bacterial isolates characteristics were spesific for K. pneumoniae.…”

Section: Bacterial Isolates Characteristicssupporting

confidence: 90%

Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

Patilaya¹,

Husori²,

Marhafanny³

2019

Open Access Maced J Med Sci

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AIM: This study was to determine the sensitivity of Klebsiella pneumonia isolated from pus specimens of post-surgery patients in Medan, Indonesia to selected antibiotics. METHODS: Samples were collected at the Laboratory of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. The isolated bacteria were identified by Gram’s stain, colony characteristics, and biochemical tests. Susceptibility of K. pneumoniae isolates were tested to selected antibiotics including amikacin, meropenem, levofloxacin, ciprofloxacin, co-trimoxazole, ceftazidime, cefoperazone, cefuroxime, cefepime, cefotaxime, tetracycline, chloramphenicol, amoxicillin and ampicillin with Kirby Bauer method by measuring the inhibitory zone. RESULTS: A total of 20 K. pneumoniae isolates were obtained in this study. The results showed that K. Pneumonia isolates exhibited good sensitivity to amikacin (100%) and meropenem (80%). Sensitivity of levofloxacin (60%), ceftazidime (55%), ciprofloxacin (55%), cefoperazone (50%), and co-trimoxazole (50%) were moderate for the bacterial isolates. K. Pneumoniae isolates indicated low sensitivity to cefuroxime (45%), chloramphenicol (35%), cefepime (30%), cefotaxime (30%), tetracycline (30%), amoxicillin (5%), and ampicillin (5%). CONCLUSION: This study concludes that K. pneumoniae isolates are most sensitive to amikacin and less sensitive to ampicillin and amoxicillin.

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Section: Bacterial Isolates Characteristicssupporting

confidence: 90%

Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

Patilaya¹,

Husori²,

Marhafanny³

2019

Open Access Maced J Med Sci

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“…varied greatly among studies (Azizi et al, 2013 (15.09%); Obaid and Khudair, 2016 (13.6% ) and El -Mashad et al, 2020 (3.84% ). Herein, we isolate K. pneumonia and K. oxytoca, both species were detected in former studies (Patel et al, 2017 andFranco et al 2019) in the contrary many previous studies did not isolate Klebsiellasp. from ovine pneumonic lungs (Garedew et al, 2010).…”

Section: Discussioncontrasting

confidence: 65%

“…coli is the most common member of family Enterobacteriacea frequently isolated from respiratory affection in sheep either from nasal swabs or from pneumonic lungs (Zaghawa et al, 2010). Also Klebsiella pneumonia and Klebsiella oxytoca of the same family are mostly associated with ovine pneumonic cases (Patel et al, 2017 andFranco et al, 2019).…”

mentioning

confidence: 99%

Mixed infection of Mycoplasma and bacteria of the respiratory tract of sheep with reference to the histopathological picture in Sharkia Governorate

2021

Egyptian Journal of Animal Health

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“…Characterization and preliminary identification of the suspected isolates of Klebsiella spp. (27) and E. coli (18,2) were done on the basis of morphology and colony characteristics. Biochemical tests were performed for identification of Klebsiella pneumoniae (12,8) and E.coli (18) isolates.…”

Section: Isolation and Identification Of Suspected Esbl Producing E mentioning

confidence: 99%

Phenotypic and molecular characterization of extended spectrum β-lactamase producingEscherichia coliandKlebsiella pneumoniaeisolates from various samples of animal origin from Assam, India

Das¹,

Borah

Sharma³

et al. 2020

Preprint

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Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae has become a major threat globally. Here we have characterized ESBL producing E. coli and K. pneumoniae from various sources, studied antibiogram and resistance gene profiles. Out of 385 samples, 31 (8.05%) were positive for ESBL producing E. coli. Such isolates could be recovered from 10.05, 8.33, 15.63, 6.67 and 4.35 per cent of cattle milk, curd, chicken, pork and cattle faeces samples, respectively. A total of 59 (15.32%) samples were positive for ESBL producing K. pneumoniae, which were isolated from 14.35, 6.25, 21.43 and 34.78 per cent cattle milk, chicken, beef and cattle faeces, respectively. All the 90 isolates were confirmed as ESBL producers by CDT and ESBL-E strip tests. Antibiogram revealed that 74.19% and 69.49% of the ESBL producing E. coli and K. pneumoniae isolates, respectively showed resistance to ceftizoxime, 25.81% and 23.73% to both co-trimoxazole and tetracycline, 19.35% and 25.42% to ciprofloxacin, 9.68% and 16.95% to chloramphenicol, 3.23% and 5.08% to pipercillin-tazobactam, and 3.23% and 3.39% to gentamicin. Resistance gene profiling showed blaCTX-M gene as most predominant (100%). The blaTEM gene was found in 54.84% and 55.93%, blaSHV gene in 90.32% and 77.97%, Sul 1 gene in 90.32% and 86.44% of ESBL producing E. coli and K. pneumoniae isolates, respectively. The Int1 gene was detected in 70.97% and 62.71% isolates, while qnrB gene was found in 3.23% and 10.17% of E. coli and K. pneumoniae isolates, respectively.

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Isolation and Identification of Klebsiella pneumoniae from Sheep-Case Report

Cited by 22 publications

References 0 publications

Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics

Mixed infection of Mycoplasma and bacteria of the respiratory tract of sheep with reference to the histopathological picture in Sharkia Governorate

Phenotypic and molecular characterization of extended spectrum β-lactamase producingEscherichia coliandKlebsiella pneumoniaeisolates from various samples of animal origin from Assam, India

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