Isolation and identification of killer yeasts from sugar cane molasses

Bonilla-Salinas, Mónica; Lappe, Patricia; Ulloa, Miguel; Garcı́a-Garibay, Mariano; Gómez-Ruiz, Lorena

doi:10.1111/j.1472-765x.1995.tb01020.x

Cited by 16 publications

(8 citation statements)

References 8 publications

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“…Fahrasmane et al ( 1988 ) isolated S. pombe from molasses, rum fermentations, soil of sugar cane plantings and soil around a rum manufacturing facility on Haiti. Additionally, S. pombe was found in 3 out of 10 sugar mills in Mexico (Bonilla‐Salinas et al 1995 ). In a more recent study on the microbiology of rum production conducted in Queensland, Australia, S. pombe was isolated from 6 out of the 16 samples of molasses taken at the same rum producing facility before clarification of the raw material (Green 2015 ).…”

Section: Discussionmentioning

confidence: 99%

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Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats

Brysch‐Herzberg

Jia

Seidel

et al. 2022

Antonie van Leeuwenhoek

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The fission yeast genus Schizosaccharomyces contains important model organisms for biological research. In particular, S. pombe is a widely used model eukaryote. So far little is known about the natural and artificial habitats of species in this genus. Finding out where S. pombe and other fission yeast species occur and how they live in their habitats can promote better understanding of their biology. Here we investigate in which substrates S. pombe, S. octosporus, S. osmophilus and S. japonicus are present. To this end about 2100 samples consisting of soil, tree sap fluxes, fresh fruit, dried fruit, honey, cacao beans, molasses and other substrates were analyzed. Effective isolation methods that allow efficient isolation of the above mentioned species were developed. Based on the frequency of isolating different fission yeast species in various substrates and on extensive literature survey, conclusions are drawn on their ecology. The results suggest that the primary habitat of S. pombe and S. octosporus is honeybee honey. Both species were also frequently detected on certain dried fruit like raisins, mango or pineapple to which they could be brought by the honey bees during ripening or during drying. While S. pombe was regularly isolated from grape mash and from fermented raw cacao beans S. octosporus was never isolated from fresh fruit. The main habitat of S. osmophilus seems to be solitary bee beebread. It was rarely isolated from raisins. S. japonicus was mainly found in forest substrates although it occurs on fruit and in fruit fermentations, too.

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Section: Discussionmentioning

confidence: 99%

“…Killer activities against S. cerevisiae and C. glabrata were described for some S. pombe strains (Bonilla‐Salinas et al 1995 ). Thus, it cannot be excluded that S. pombe could suppress proliferation of S. octosporus to a certain degree.…”

Section: Discussionmentioning

confidence: 99%

Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats

Brysch‐Herzberg

Jia

Seidel

et al. 2022

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

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“…The killer phenomenon is widespread among both laboratory and wild‐type yeasts of various origins and genera ( Philliskirk & Young 1975; Cuinier & Gros 1983; Rosini 1983; Ciolfi 1985; Vagnoli et al . 1993 ; Bonilla‐Salinas et al . 1995 ; Da Silva 1997).…”

Section: Introductionmentioning

confidence: 99%

Studies on strong and weak killer phenotypes of wine yeasts: production, activity of toxin in must, and its effect in mixed culture fermentation

1999

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R .A . M U SM AN N O, T. D I M AG G IO AN D G. CO R AT ZA . 1999. Two different killer phenotypes were detected among K ¦ (killer) yeasts isolated from spontaneous wine fermentations using a plate bioassay. The two phenotypes differed in their degree of killer activity, and were designated as SK ¦ (strong killer) and WK ¦ (weak killer). Strains showing either phenotype were assayed for expression of killer activity under different growth conditions. Growth in must negatively affected expression of the killer activity of both phenotypes. The supernatant fluids from must cultures showed a lower killing effect than those from yeast phosphate dextrose broth (YPDB) cultures. The ability of the two K ¦ phenotypes to prevail on K-sensitive yeasts was studied in mixed-culture fermentation experiments. Under these conditions, only strains showing SK ¦ phenotype were able to prevail on the K-sensitive yeasts. These results suggest that the K ¦ phenotype could play a relevant role in spontaneous fermentations provided that the strain exhibits an SK ¦ phenotype, and that the latter phenotype should be preferred when selected K ¦ strains are to be used as fermentation starters.

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“…The killer phenomenon occurs in such natural substrates as fruits, mushrooms, decaying plant materials, soil [3], fermentations [4–7], sugar cane molasses [8], aquatic environments [9, 10], olive brines [11] and maize kernels [12]. This phenomenon may play an important role in the ecology of yeasts.…”

Section: Introductionmentioning

confidence: 99%

Killer activity at different pHs against Cryptococcus neoformans var. neoformans serotype A by environmental yeast isolates

Criseo

Gallo

Pernice

1999

Mycoses

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Yeast isolates that share the same habitats as Cryptococcus neoformans var. neoformans serotype A in a restricted Mediterranean area were assayed in order to verify their killer activity against Cr. neoformans strains isolated from clinical and environmental sources. Many of the environmental yeast isolates expressed the killer phenomenon against the assayed strains of Cr. neoformans. Two species of Candida: Candida parapsilosis and Candida famata, and Pichia carsonii, were the most active killers at pH 4.6, 5.0 and 5.6 levels encountered in pigeon and canary guanos. Killer activity by C. parapsilosis is reported for the first time. The authors hypothesized that the killer phenomenon exerted by yeast species with heavy killer activity against Cr. neoformans would lend themselves for use as biological control agents against sensitive strains of Cr. neoformans when directly inoculated into the habitats of Cr. neoformans.

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Isolation and identification of killer yeasts from sugar cane molasses

Cited by 16 publications

References 8 publications

Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats

Insights into the ecology of Schizosaccharomyces species in natural and artificial habitats

Studies on strong and weak killer phenotypes of wine yeasts: production, activity of toxin in must, and its effect in mixed culture fermentation

Killer activity at different pHs against Cryptococcus neoformans var. neoformans serotype A by environmental yeast isolates

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